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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 640-646.doi: 10.3724/SP.J.1006.2009.00640

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Analyses on Rs-AFP2 Transgenic Wheat Plants and Their Resistance to Rhizoctonia cerealis

LU Yan12,ZHANG Zeng-Yan1*,REN Li-Juan3,LIU Bao-Ye1,LIAO Yong14,XU Hui-Jun1,DU Li-Pu1,MA Hong-Xiang3,REN Zheng-Long4,JING Jin-Xue2*,XIN Zhi-Yong1   

  1. 1National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, chinese Academy of Agricultural Sciences,Beijing 100081,China;2College of Plant Protection,Northwest A&F University,Yangling 712100,China;3Biotechnology Research Institute,Jiangsu Academy of Aaricultural Sciences,Nanjing 210014,China;4College of Agronomy,Sichuan Agricultural University,Ya'an 625000,China
  • Received:2008-10-16 Revised:2008-12-04 Online:2009-04-12 Published:2009-02-13
  • Contact: ZHANG Zeng-Yan

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Abstract:

In this study, the gene encoding Rs-AFP2, a small cyteine-rich antifungal protein from radish, was evidenced to be transformed into a wheat (Triticum aestivum L.) cultivar Yangmai 12 via bombardment of biolistic particle and PCR detection. To evaluate if expression of Rs-AFP2 enhances the transgenic wheat resistance to Rhizoctonia cerealis, a major pathogen of wheat sharp eyespot, the transgenic wheat plants from T1 to T4 generations were subjected to R. cerealis inoculation and the disease resistance rating, and PCR, PCR-Southern, Southern blotting, and RT-PCR/Q-RT-PCR analyses for the Rs-AFP2 transgene. Results showed that Rs-AFP2 gene was integrated as a single copy into the susceptible receptor wheat cultivar Yangmai 12, inherited from T1 to T4, and expressed in the wheat background. The transgenic wheat plants expressing Rs-AFP2 showed enhanced resistance to R. cerealis and unchanged agronomic traits compared with nontransgenic Yangmai 12. In the transgenic wheat plants, the express level of Rs-AFP2 was associated with the disease resistance degree. These results suggested that Rs-AFP2 gene can be useful for improving wheat resistance to R. cerealis.

Key words: Antimicrobial peptides, Rs-AFP2, Transgenic wheat, Gene expression, Resistance to Rhizoctonia cerealis

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