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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1344-1349.doi: 10.3724/SP.J.1006.2009.01344

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Phosphorus Deficiency on Growth and Nitrogen Fixation of Soybean after Nodule Formation

MIAO Shu-Jie,QIAO Yun-Fa,HAN Xiao-Zeng*,WANG Shu-Qi,LI Hai-Bo   

  1. Northeast Institute of Geography and Agro-exology,Chinese Academy of Sciences,Harbin 150081,China
  • Received:2008-12-20 Revised:2009-03-18 Online:2009-07-12 Published:2009-05-19
  • About author:E-mail: miaoshujie@126.com; Tel: 0451-86648128

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Abstract:

Phosphorus had a key role in soybean growth, however, it is very different to separate the role in soybean growth from that in nodule function. The research was conducted to elucidate the effects of phosphorus deficiency on growth and nitrogen fixation after nodule formation in soybean. Soybean seedlings were cultivated in nutrient solution culture with normal phosphorus concentration (30 µmol L-1), which was shifted to 0 and 4 µmol L-1 after nodule formation resulting in phosphorus deficienct for soybean plants.The results showed that phosphorus deficiency negatively influenced soybean plant growth at the ninth day of phosphorus deficiency treatment and nitrogen fixation at the beginning of the treatnent. The nodule number increased by 11.8%, 15.4%, and 20.0%, respectively in 0, 4, and 30 µmol L-1 phosphorus treatments at the ninth of treatment (DOT) compared with that at the third DOT. Leghemoglobin concentration per gram of plant and per nodule biomass increased with phosphorus concentration increase. These suggested that phosphorus deficiency affected significantly nodule development and nitrogen fixation when soybean plants formed sufficient nodule.

Key words: Soybean, Phosphorus, Nodule, Nitrogen fixation

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