Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1729-1737.doi: 10.3724/SP.J.1006.2009.01729

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

QTL Mining for Sheath Blight Resistance Using the Backcross Selected Introgression Lines for Grain Quality in Rice

LI Fang1,CHENG Li-Rui1,XU Mei-Rong1,ZHOU Zheng1,ZHANG Fan1,SUN Yong1,ZHOU Yong-Li1,ZHU Ling-Hua1,XU Jian-LOng1*,LI Zhi-Kang1,2   

  1. 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2International Rice Research Institute,DAPO Box7777,Metro Manila,Philippines
  • Received:2009-02-05 Revised:2009-03-20 Online:2009-09-12 Published:2009-07-04
  • Contact: XU Jian-ling,E-mail: xujl@caas.net.cn

Abstract:

QTLs for disease scale (DS), relative lesion height (RH) and plant height (PH) were mapped using the four introgression lines selected against grain quality from the four introgression populations between two elite varieties, IR64 and Teqing as recurrent parents and two tall varieties, Tarom Molaii and Binam with high grain quality and sheath blight resistance (SBR) as donors. DS had high significant positive correlation with RH, and both of them were significantly negatively correlated with PH. DS, RH and PH presented wide segregations even in the relative small (2860) introgression populations, including some semidwarf plants segregated with overparent SBR. Ten, eight, eight and six QTLs for the three traits were identified in IR64/Tarom Molaii, Teqing/Tarom molaii, IR64/Binam and Teqing/Binam, respectively by one   way ANOVA analysis. The two donor alleles at most QTLs reduced DS and RH as well as increased PH. No any a common QTL for each of the three traits was detected in the populations of the same donor in the two different background, indicating there was a obvious genetic background effect on expression of SBR- and PH-QTLs. QTLs linked to the same marker loci between PH and DS, and PH and RH accounted for 38% and 52% of total QTLs for the two traits, respectively, indicating SBR had tight relationship with PH and both of them shared some linked loci. As compared with the previous QTL mapping results of grain quality in the same populations, most QTLs for grain quality and SBR distributed in different chromosome regions and showed independently inherited. Efficiency, influencing factors and its application in breeding of QTL mapping for non-target traits using introgression lines selected against the target trait were deeply discussed, and importance of selective introgression lines in QTL mining and breeding use was also emphasized.

Key words: Backcross selected introgression lines(SBILs), Quantiative trait locus(QTL), Rice sheath blight resistance(SBR), Grain quality, Plant height

[1] McKenzie K S, Rush M C, Groth D E. Registration of two disease-resistant germplasm lines of rice. Crop Sci, 1986, 26: 839-840

[2] Marchetti M A, Bollich C N. Quantification of the relationship between sheath blight severity and yield loss in rice. Plant Dis, 1991, 75: 773-775

[3] Toriyama K. Breeding for resistance to major rice diseases in Japan. Los Baños, Philippines: IRRI, 1972. 253-281

[4] Dasgupta M K. Rice sheath blight: the challenge continues. In: Singh U S, Mukhopadhyay A N, Kumar J, Chaube H S, eds. Plant Diseases of International Importance Vol I. Diseases of Cereals and Pulses. Prentice Hall, Englewood Cliff, New Jersey. 1992

[5] Rush M C, Lindberg G D. Rice disease research. Rice J, 1996, 77: 49-52

[6] Xie Q J, Rush M C, Cao J. Somaclonal variation for disease resistance in rice (Oryza sativa L.). In: Grayson B T, Green M B, Copping L G, eds. Pest Management in Rice. New York: Elsevier Applied Science, 1990. pp 491-509

[7] Chang T T. The present status of breeding for resistance to rice blast and sheath blight in Taiwan. Int Rice Res Newsl, 1986, 11: 1-7

[8] Che K P, Zhan Q C, Xing Q H, Wang Z P, Jin D M, He D J, Wang B . Tagging and mapping of rice sheath blight resistant gene. Theor Appl Genet, 2003, 106: 293-297

[9] Kunihiro Y(国广泰史), Qian Q(钱前), Sato H(佐藤宏之), Teng S(滕胜), Zeng D-L(曾大力), Fujimoto K(藤本宽), Zhu L-H(朱立煌).QTL analysis of sheath blight resistance in rice. Acta Genet Sin (遗传学报), 2002, 29(1): 50-55 (in Chinese with English abstract)

[10]Han Y-P(韩月澎), Xing Y-Z(邢永忠), Chen Z-X(陈宗祥), Gu S-L(顾世梁), Pan X-B(潘学彪), Chen X-L(陈秀兰), Zhang Q-F(张启发). Mapping QTLs for horizontal resistance to sheath blight in an elite rice restorer line, Minghui 63. Acta Genet Sin (遗传学报), 2002, 29(7): 622-626 (in Chinese with English abstract)

[11]Pan X-B(潘学彪), Zou J-H(邹军煌), Chen Z-X(陈宗祥), Lu J-F(陆驹飞), Yu H-X(于恒秀), Li H-T(李海涛), Wang Z-B(王子斌), Rush M C, Zhu L-H(朱立煌). Mapping major-QTL for resistance to sheath blight in rice, Jamine 85. Chin Sci Bull (科学通报), 1999, 44(15): 1629-1635(in Chinese)

[12]Pinson S R M, Capdevielle F M, Oard J H. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines. Crop Sci, 2005, 45: 503-510.

[13]Sato H, Ideta O, Audo I, Kunihiro Y, Hirabayashi H, Iwano M, Miyasaka A, Nemoto H, Imbe T. Mapping QTLs for sheath blight resistance in the rice line Wss2. Breed Sci, 2004, 54: 265-271

[14]Zou J H, Pan X B, Chen Z X, Xu J Y, Lu J F, Zhai W X, Zhu L H. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet, 2000, 101: 569-575

[15]Li Z K, Pinson S R M, Marchetti M A, Stansel J W, Park W D. Characterization of quantitative trait loci (QTLs) in cultivated rice controlling to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet, 1995, 91: 382-388

[16]Yin Y-J(殷跃军), Zuo S-M(左示敏), Wang H(王辉), Chen Z-X(陈宗祥), Ma Y-Y(马玉银), Zhang Y-F(张亚芳), Gu S-L(顾世梁), Pan X-B(潘学彪). Pyramiding effects of three quantitative trait loci for resistance to sheath blight using near-isogenic lines of rice. Chin J Rice Sci (中国水稻科学), 2008, 22(4): 340-346 (in Chinese with English abstract)

[17]Zuo S-M(左示敏), Yin Y-J(殷跃军), Zhang L(张丽), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Pan X-B(潘学彪). Breeding value and further mapping of a QTL qsB-11 conferring the rice sheath blight resistance. Chin J Rice Sci (中国水稻科学), 2007, 21(2): 136-142(in Chinese with English abstract)

[18]Tanksley S D, Nelson J C. Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet, 1996, 92: 191-203

[19]Zhao X Q, Xu J L, Zhao M, Lafitte R, Zhu L H, Fu B Y, Gao Y M, Li Z K. QTLs affecting morph-physiological traits related to drought tolerance detected in overlapping introgression lines of rice (Oryza sativa L.). Plant Sci, 2008, 174: 618-625

[20]Xu J L, Lafitte H R, Gao Y M, Fu B Y, Torres R, Li Z K. QTLs for drought escape and tolerance identified in a set of random introgression lines of rice. Theor Appl Genet, 2005, 111: 1642-1650

[21]Zhang X, Zhou S X, Fu Y C, Su Z, Wang X K, Sun C Q. Identification of a drought tolerant introgression line derived from Dongxiang common wild rice (O. rufipogon Griff.). Plant Mol Biol, 2006, 62: 247-259

[22]Arif M. Moleuclar Mapping of Genes/QTLs Affecting Resistance to Xanthomonas Oryza pv. oryza and Grain Quality Traits in Rice (Oryza sativa L.). PhD Dissertation of University of Philippines, Los Baños, Philippines. 2002

[23]Chen Z-X(陈宗祥), Zou J-H(邹军煌), Han Y-P(韩月鹏), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Yu H-X(于恒秀), Zhang Y-F(张亚芳), Pan X-B(潘学彪). An innovated method for research on inheritance of resistance to rice sheath blight and its verification. Chin J Rice Sci (中国水稻科学), 2002, 16(1): 74-76 (in Chinese with English abstract)

[24]Rush M C, Hoff B J, Mcllrath W O. A uniform disease rating system for rice disease in the United States. Proc 16th Rice Tech Working Group, Lake Charles, 1976. p 64

[25]Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential. Genome Res, 2001, 11: 1446-1448

[26]SAS Institute. SAS/STAT User’s Guide. Cary: SAS Institute, 1996

[27]Zang J P, Sun Y, Wang Y, Yang J, Li F, Zhou Y L, Zhu L H, Reys J, Fotokian M, Xu J L, Li Z K . Dissection of genetic overlap of salt tolerance QTLs at the seeding and tillering stages using backcross introgressive lines in rice. Sci China (Ser C·Life Sci), 2008, 51(7): 583-591

[28]Chevin L M, Hospital F. Selective sweep at a quantitative trait locus in the presence of background genetic variation. Genetics, 2008, 180: 1645-1660

[29]Ungerer M C, Linder C R, Rieseberg L H. Effects of genetic background on response to selection in experimental populations of Arabidopsis thaliana. Genetics, 2003, 163: 277-286

[30]Xie X-W(谢学文), Xu M-R(许美容), Zang J-P(藏金萍), Sun Y(孙勇), Zhu L-H(朱苓华), Xu J-L(徐建龙), Zhou Y-L(周永力), Li Z-K(黎志康). Genetic background and environment effects on expression of QTL for sheath blight resistance in reciprocal introgression lines of rice. Acta Agron Sin (作物学报), 2008, 34(11): 1885-1893 (in Chinese with English abstract)

[31]Yuan X-P(袁筱萍), Wei X-H(魏兴华), Yu H-Y (余汉勇), Wang Y-P(王一平), Tang S-X(汤圣祥). Effects of different cultivars and relatives factors on sheath blight resistance of rice. Acta Agron Sin (作物学报), 2004, 30(4): 739-744 (in Chinese with English abstract)

[32]Rush M C, Pan X B, Sha X Y. Development of sheath blight resistance in rice. In: 85thAnnu Res Rep, Rice Research Station, LSU Agricultural Center, Crowley, LA, USA, 1995, 85: 380-393

[33]Han Y P, Xing Y Z, Gu S L, Chen Z X, Pan X B, Chen X L. Effect of morphological traits on sheath blight resistance in rice. Acta Bot Sin, 2003, 45: 825-831

[34]Ware D, Jaiswal P, Ni J, Pan X, Chang K, Clark K, Teytelman L, Schmidt S, Zhao W, Cartinhour S, McCouch S, Stein L. Gramene: A resource for comparative grass genomics. Nucl Acids Res, 2002, 30: 103-105

[35]Martin T J, Ellingboe A H. Differences between compatible parasite/host genotypes involving the Pm4 locus of wheat and the corresponding genes in Erysiphe graminis f. sp. tritici. Phytopathology, 1976, 66: 1435-1438

[36]Royer M H, Nelson R R, MacKenzie D R, Diehle D A. Partial resistance of near-isogenic wheat lines compatible with Erysiphe graminis f. sp. tritici. Phytopathology, 1984,69: 405-409

[37]Li Z K, Luo L J, Mei H W, Paterson A H, Zhao X H, Zhong D B, Wang Y P, Yu X Q, Zhu L, Tabien R, Stansel J W, Ying C S. A ‘defeated’ rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv. oryzae. Theor Appl Genet, 1999, 261: 58-63

[38]Li Z-K(黎志康). Strategies for molecular rice breeding in China. Mol Plant Breed (分子植物育种), 2005, 3(5): 603-608 (in Chinese with English abstract)

[39]Xu J-L(徐建龙), Gao Y-M(高用明), Fu B-Y(傅彬英), Li Z-K(黎志康). Identification and screening of favorable genes from rice germplasm in backcross introgression populations. Mol Plant Breed (分子植物育种), 2005, 3(5): 619-628 (in Chinese with English abstract)

[40]Li Z K, Fu B Y, Gao Y M, Xu J L, Ali J, Lafitte H R, Jiang Y Z, Rey J, Dominggo, Vijayakumar C H M, Maghirang R, Zheng T Q, Zhu L H.Genome-wide introgression lines and a forward genetics strategy for functional genomic research of complex phenotypes in rice. Plant Mol Biol, 2005, 59: 33-52

[41]Ali A J, Xu J L, Ismail A M, Fu B Y, Vijaycumar C H M, Gao Y M, Domingo J, Maghirang R, Yu S B, Gregorio G, Yanaghihara S, Cohen M, Carmen B, Mackill D, Li Z K. Hidden diversity for abiotic and biotic stress tolerances in the primary gene pool of rice revealed by a large backcross breeding program. Field Crops Res,2006, 97: 66-76

[42]Zheng T-Q(郑天清), Xu J-L(徐建龙), Fu B-Y(傅彬英), Gao Y-M(高用明), Veruka S, Lafitte R, Zhai H-Q(翟虎渠), Wan J-M(万建民), Zhu L-H(朱苓华), Li Z-K(黎志康). Preliminary identification of genetic overlaps between sheath blight resistance and drought tolerance in the introgression lines from directional selection . Acta Agron Sin (作物学报), 2007, 33(8): 1380-1384 (in Chinese with English abstract)

Kang L(康乐), Li H(李宏), Sun Y(孙勇), Lu D-C(卢德城), Zhang F(张帆), Huang D-Q(黄道强), Xu J-L(徐建龙), Wang Z-D(王志东), Zhu L-H(朱苓华), Gao Y-M(高用明), Fu B-Y(傅彬英), Li K-H(李康活), Zhou Y-L(周永力), Zhou S-C(周少川), Li Z-K(黎志康). Genetic dissection of yield potential in rice (Oryza sativa L.) using introgression lines. Acta Agron Sin (作物学报), 2008, 34(9): 1500-1509 (in Chinese with English abstract)

[1] Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling. Genome-wide association analysis and prediction model construction for soybean plant height [J]. Acta Agronomica Sinica, 2026, 52(6): 1743-1756.
[2] DONG Wei-Jin, ZHANG Ya-Feng, LI Qi-Yun, LU Yang, ZHANG Zheng-Kun, SUI Li. Effects of Beauveria bassiana colonization on maize growth and yield under elevated CO2 concentration [J]. Acta Agronomica Sinica, 2025, 51(7): 1874-1886.
[3] ZHENG Hao-Fei, YANG Nan, DU Jian, JIA Gai-Xiu, ZOU Yue, MA Wen-Hao, WANG Yan-Ting, SUO Dong-Rang, ZHAO Jian-Hua, SUN Ning-Ke, ZHANG Jian-Wen. Long-term combined application of organic and inorganic fertilizers achieving high yield and high quality of maize in northwest irrigated oasis [J]. Acta Agronomica Sinica, 2025, 51(6): 1618-1628.
[4] GUO Xu-Hu, LI Ling-Zhi, LI Feng, MA Bo-Yan, JIA Xiao-Yu. Functional study on the regulation of plant architecture by tomato type I MADS-box gene SlMADS79 [J]. Acta Agronomica Sinica, 2025, 51(4): 982-991.
[5] XU Jian-Xia, DING Yan-Qing, CAO Ning, CHENG Bin, GAO Xu, LI Wen-Zhen, ZHANG Li-Yi. Genome-wide association analysis and prediction of candidate genes for plant height and internode number in Chinese sorghum [J]. Acta Agronomica Sinica, 2025, 51(3): 568-585.
[6] LI Xiang-Yu, JI Xin-Jie, WANG Xue-Lian, LONG An-Ran, WANG Zheng-Yu, YANG Zi-Hui, GONG Xiang-Wei, JIANG Ying, QI Hua. Effects of straw returning combined with nitrogen fertilizer on yield and grain quality of spring maize [J]. Acta Agronomica Sinica, 2025, 51(3): 696-712.
[7] QIN Jin-Hua, HONG Wei-Yuan, FENG Xiang-Qian, LI Zi-Qiu, ZHOU Zi-Yu, WANG Ai-Dong, LI Rui-Jie, WANG Dan-Ying, ZHANG Yun-Bo, CHEN Song. Analysis of agronomic and physiological indicators of rice yield and grain quality under nitrogen fertilization management [J]. Acta Agronomica Sinica, 2025, 51(2): 485-502.
[8] ZHAO Hai-Hong, LI Meng-Yuan, LIU Jin-Jing, WANG Yuan-Yuan, DU Lei, WANG Juan, DONG Cheng-Guang, LI Cheng-Qi. Detection of QTNs and QTN-by-environment interactions for plant height in upland cotton (G. hirsutum L.) using the 3VmrMLM method [J]. Acta Agronomica Sinica, 2025, 51(10): 2619-2631.
[9] LIU Wei, WANG Yu-Bin, LI Wei, ZHANG Li-Feng, XU Ran, WANG Cai-Jie, ZHANG Yan-Wei. Overexpression of soybean isopropyl malate dehydrogenase gene GmIPMDH promotes flowering and growth [J]. Acta Agronomica Sinica, 2024, 50(3): 613-622.
[10] WU Hao, ZHANG Ying, WANG Chen, GU Han-Zhu, ZHOU Tian-Yang, ZHANG Wei-Yang, GU Jun-Fei, LIU Li-Jun, YANG Jian-Chang, ZHANG Hao. Effects of cultivation optimization on root characteristics and starch properties of rice at grain filling stage in the lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2024, 50(2): 478-492.
[11] DIAO Xian-Min, WANG Li-Wei, ZHI Hui, ZHANG Jun, LI Shun-Guo, CHENG Ru-Hong. Development, genetic deciphering, and breeding utilization of dwarf lines in foxtail millet [J]. Acta Agronomica Sinica, 2024, 50(2): 265-279.
[12] YANG Shi-Jie, WANG Hua-Zhi, PAN Yi-Min, HUANG Rui, HOU Sen, QIN Hui-Bin, MU Zhi-Xin, WANG Hai-Gang. Genome-wide association analysis for plant height in foxtail millet (Setaria italica L.) germplasm resources in Shanxi, China [J]. Acta Agronomica Sinica, 2024, 50(12): 2984-2997.
[13] ZHAO Yang, LI Long, YANG Jin-Wen, JING Rui-Lian, SUN Dai-Zhen, WANG Jing-Yi. An E3 ubiquitin ligase gene TaSINA-3A is associated with plant height and 1000-grain weight in various environments in wheat [J]. Acta Agronomica Sinica, 2024, 50(10): 2654-2664.
[14] YANG Chen-Xi, ZHOU Wen-Qi, ZHOU Xiang-Yan, LIU Zhong-Xiang, ZHOU Yu-Qian, LIU Jie-Shan, YANG Yan-Zhong, HE Hai-Jun, WANG Xiao-Juan, LIAN Xiao-Rong, LI Yong-Sheng. Mapping and cloning of plant height gene PHR1 in maize [J]. Acta Agronomica Sinica, 2024, 50(1): 55-66.
[15] ZHANG Diao-Liang, YANG Zhao, HU Fa-Long, YIN Wen, CHAI Qiang, FAN Zhi-Long. Effects of multiple cropping green manure on grain quality and yield of wheat with different irrigation levels [J]. Acta Agronomica Sinica, 2023, 49(9): 2572-2581.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!