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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 313-320.doi: 10.3724/SP.J.1006.2010.00313

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of RNA Interference of SBE3 Gene Expression on Starch Accumulation and Key Enzymes Activities Involved in Starch Synthesis in Transgenic Rice Grain

WANG Jie-Ming,ZHANG Jian,JIANG Hai-Yang,ZHU Su-Wen,FAN Jun,CHENG Bei-Jiu*
  

  1. Anhui Agricultural University, Anhui Provincial Key Laboratory of Crop Biology, Hefei 230036, China
  • Received:2009-06-11 Revised:2009-08-31 Online:2010-02-10 Published:2009-12-21
  • Contact: CHANG Bei-Jiu, E-mail: cbj@ahau.edu.cn
  • Supported by:

    本研究由国家高技术研究发展计划(863计划)项目(2008AA10Z408,2006AA10Z1B4)资助。

Abstract:

Rice cultivar “Zhonghua 11” (control) and its transgenic line were used as the experimental materials. The expression level of SBE3 gene, some related key enzymes activities, and the contents of amylose and amylopectin in different developmental stages of rice grain were measured. Results showed that the SBE3 gene expression level and the SBE activity were distinctly reduced by RNA interference (RNAi), but a little difference was observed between the two transgenic lines. The peak values of SBE activity reached three days earlier in transgenic rice than in non-transgenic control. RNAi of the SBE3 gene expression level also obviously reduced the enzyme activities of ADPG-PPase, SSS and DBE during different grain developmental stages, especially, the peak values of activities ofADPG-PPaseandSSS decreased to the largest extent. In addition, the content of amylose was significantly higher in different developmental stages in transgenic rice grain than that in the control, while thousand-grain weight was significantly reduced in mature grain, the general trend was that the higher the amylose content, the smaller the grain weight.

Key words: Rice, RNA interference, Starch branching enzyme3, Starch accumulation, Enzyme activity

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