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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1853-1863.doi: 10.3724/SP.J.1006.2010.01853

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phylogenetic Relationships of Sugarcane Related Genera and Species Based on ITS Sequences of Nuclear Ribosomal DNA

LIU Xin-Long1,SU Huo-Sheng1,MA Li1,LU Xin1,YING Xiong-Mei1,CAI Qing1,2,FAN Yuan-Hong1,*   

  1. 1 Yunnan Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China; 2 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2010-03-16 Revised:2010-06-27 Online:2010-11-12 Published:2010-08-30
  • Contact: FAN Hong-Li,E-mail:fyhysri@vip.sohu.com

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Abstract: Sugarcane related genera and species are important germplasm resources for sugarcane breeding and germplasm innovation. To effectively collect these wild resources and utilize them, ITS (Internal Transcribed Spacer) sequence of 120 accessions which belong to eight genera and thirty seven species were used to analyze their genetic relationships and construct phylogentic relationships with P. purpureum as an outgroup. The results showed the sequence lengths of ITS1, ITS2 and 5.8sDNA of all accessions were 204–208 bp, 215–220 bp, and 164 bp respectively, and their variable sites were 91, 93, and 18, the informative sites were 70, 68, and 9, the GC content was 60.4%–69.1%, 66.1%–73.4% and 54.1%–58.0%. According to the ratio of the variable sites and informative sites to all sites, ITS sequence was richer in variances than 5.8sDNA sequence, and the variances of ITS1 sequence were richer than those of ITS2’s. The genetic distance analysis between sugarcane related genera and species indicated that Miscanthus and Triarrhena were the closest to Saccharum, and the closer ones were Erianthus and Narenga, so the species from these genera should be more considered on collecting sugarcane wild resources in the future; Microstegium, Spodiopogon and Imperata represented farther relationships with Saccharum. The sugarcane related genera and species were separated into ten groups according to the phylogenetic tree of neighbor-joining and maximum parsimony. According to their phylogenetic relationships, E. arundinaceus should be separated from Saccharum and clastified as Erianthus species; Triarrhena shoud be included in Miscanthus; the two species of Narenga did not remain in the same group, but N. porphyrocoma and E. rockii were clustered into the same group, and N. fallax did not belong to any groups, so further researches are needed in identifying the two species. These species from Erianthus and Miscanthus were put in different groups, indicating the very complex genetic relationships within Erianthus and Miscanthus, their species possess big genetic differences. Four samples were found error in classifying species, so the application of their ITS sequences should be avoided. 

Key words: Sugarcane, ITS, Phylogeny, Saccharinae

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