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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1398-1405.doi: 10.3724/SP.J.1006.2011.01398

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTLs for Lignin Content of Seed Coat in Brassica napus L. in Different Environments

QU Cun-Min1,FU Fu-You2,LU Kun1,XIE Jing-Mei1,LIU Xiao-Lan1,HUANG Jie-Heng1,LI Bo1,WANG Rui1,CHEN Li1,TANG Zhang-Lin1,LI Jia-Na1,*   

  1. 1 Chongqing Rapeseed Technology Research Center of Southwest University, Chongqing 400716, China; 2 Plant Gene Resources of Canada, Agriculture and Agri-Food Canada, Saskatoon SK, S7N 0X2 Canada
  • Received:2010-12-05 Revised:2011-03-06 Online:2011-08-12 Published:2011-05-11
  • Contact: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68251950 E-mail:lion4302@163.com

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Abstract: The objective of this study was to identify QTLs for lignin content of seed coat in Brassica napus using the composite interval mapping (CIM) method. The recombinant inbred lines (RIL) population derived from a cross between black-seeded male parent cultivar Zhongyou 821 and yellow-seeded female parent line GH06 was established by selfing for seven successive generations with single seed propagating from F2. The population was grown at Beibei and Wanzhou in Chongqing in 2007. The QTLs of seed coat lignin content in two environments were detected using the genetic map constructed in 2007. A total of 12 QTLs for seed coat lignin content were located on the four linkage groups, each of them explained 4.50–8.79% of phenotypic variation. One QTL located on linkage group (LG) 3 was linked with the same marker EM19ME23/130 in two environments, other QTLs had different locations between two environments. The QTLs found in Beibei were mainly distributed on LG 20, and the QTLs detected in Wanzhou mainly distributed on LG 3. The QTLs for flavoid contents in embryo and seed color were located in the nearly region with some QTLs for seed coat lignin content. The correlation analysis showed the lignin content of seed coat had significantly and positively correlated with flavoid content of embryo in different environments. In conclution, (1) the seed coat lignin content is controlled by many minor-effect genes, with a genetic pattern of quantitative trait, and the expression of the QTL is affected by environmental factors greatly; (2) the syntheses of lignin in seed coat and flavoid of embryo are probably controlled by the same genes or have the same pathway partially.

Key words: Brassica napus L, Seed coat, Lignin content, Quantitative trait locus (QTL), Recombinant inbred lines (RILs)

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