施氮时期对小麦籽粒HMW-GS积累及GMP粒度分布的影响

doi:10.3724/SP.J.1006.2011.02241

Abstract

Abstract: Wheat processing quality has close relationships with high molecular weight glutenin subunits (HMW-GS) and glutenin macropolyer (GMP) in grains. Nitrogen fertilization scheme could affect the accumulation of HMW-GS and the size distribution of GMP. In this study, we used a strong gluten winter wheat cultivar, Jinan 17, and a weak gluten winter wheat cultivar, Lumai 21, to investigate the effects of nitrogen topdressing stage on the accumulation of HMW-GS and the size distribution of GMP in grains. The nitrogen fertilizer rate during the whole growth period was 225 kg ha^-¹, of which a half was applied as base fertilizer and the other half was topdressed at pseudo stem erection (SE, GS 30), jointing (JT, GS 32), or booting (BT, GS 41) stage. The spike samples were collected from 7 d after anthesis (DAA) to maturity with a 7-day interval. The HMW-GS in grains formed before 14 DAA, and Jinan 17 had higher contents of HMW-GS and GMP than Lumai 21, which indicated that more glutenin is accumulated in the strong gluten cultivar than in the weak gluten cultivar. At maturity, the contents of HMW-GS and GMP in Jinan 17 showed decreasing trends with the delay of topdressing practice, and the highest values were observed in the SE treatment. In Lumai 21, compared to the SE and BT treatments, the JT treatment significantly accelerated the HMW-GS accumulation at middle-late stage of grain filling, and the rapid accumulation period was prolonged. At middle-late stage of grain filling, the accumulation rates of x-type subunits (1, 4, 5, and 7) were increased significantly in the SE treatment of Jinan 17 and the JT treatment of Lumai 21, whereas the y-type subunits were seldom affected by nitrogen application timging. For both cultivars, delay of nitrogen topdressing enhanced the percentages of volume and surface of GMP particle with diameter less than 12 μm, but reduced those of GMP particle with diameter larger than 100 µm. The percentage of GMP particle number with diameter larger than 12 μm was increased in Jinan 17 with the delay of nitrogen topdressing but decreased in Lumai 21.The percentage of large GMP particle volume was higher in Jinan 17 (containing subunit pair 4+12) than in Lumai 21 (containing subunit pair 5+10). This result suggested that polymerization of subunits and GMP particles are related to not only the subunit type but also the subunit content per unit of flour.

Key words: Wheat, Nitrogen fertilization stage, High molecular weight glutenin subunits (HMW-GS), Glutenin macropolyer (GMP)

CAO Li, WANG Zhen-Lin, DAI Zhong-Min, YIN Yan-Ping, DI Hua-Xu, NI Yang-Li, CA Tie, LI Yong, WANG Beng, CHEN Er-Ying, GUO Dun-Xiang, CHEN Xiao-Guang. Effect of Nitrogen Fertilization Timing on HMW-GS Accumulation and GMP Size Distribution in Wheat Grains[J].Acta Agron Sin, 2011, 37(12): 2241-2250.

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Effect of Nitrogen Fertilization Timing on HMW-GS Accumulation and GMP Size Distribution in Wheat Grains

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