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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1347-1353.doi: 10.3724/SP.J.1006.2012.01347

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Genetic Analysis and Gene Mapping of Early Senescence Leaf Mutant esl2 in Rice

XU Fang-Fang,SANG Xian-Chun,REN De-Yong,TANG Yan-Qiang,HU Hong-Wei,YANG Zheng-Lin,ZHAO Fang-Ming,HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400716, China
  • Received:2012-01-05 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04
  • Contact: 何光华, E-mail: hegh@swu.edu.cn E-mail:xff806954@163.com

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Abstract: An early senescence leaf mutant temporarily designated as esl2was discovered in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by ethyl methane sulfonate (EMS). Themutant kept normal at seedling stage and showed etiolated senescence leaves from booting sage to the maturity, especially at tip and margin of leaf blade. By contrast with the wild type, photosynthetic pigment contents decreased significantly at both booting stage and heading stage, superoxide dismutase (SOD) activity and soluble protein contents decreased significantly, while the activities of peroxidase (POD) and catalase (CAT), and contents of reactive oxygen species (ROS), malondialdehyde (MDA) and praline increased significantly at heading stage in the esl2 mutant. The assay of Transmission Electronic Microscope (TEM) observation was performed and demonstrated that the most cells appeared irregular and void with endolysis and disrupted organelles at senescence part of the mutational leaf, concomitantly, abnormal chloroplast appeared and showed dissolved membrane, indistinct grana, disorganized lamellar structure and incorrect thylakoid. Genetic analysis suggested that the mutational trait was controlled by one nuclear recessive gene. Xinong 1A was crossed with the esl2 and 1005 mutational F2single plants were used for gene mapping. Finally, Esl2 locus was mapped between SSR marker RM17122 and swu4-13 on the chromosome 4 with physical distance of 244 kb. This result provides a foundation of Esl2 gene cloning by map-based strategy as well as its functional analysis.

Key words: Rice (Oryza sativa L.), Early senescence, Gene mapping

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