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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 1119-1126.doi: 10.3724/SP.J.1006.2013.01119

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cDNA-SCoT Analysis of Differentially Expressed Genes in Sugarcane Induced by Leifsonia xyli subsp. xyli

CHEN Ming-Hui1,ZHANG Bao-Qing1,SONG Xiu-Peng1,CHEN Hu3,YANG Li-Tao1,2,*,LI Yang-Rui1,2*,CHEN Bao-Shan1   

  1. 1 Guangxi University / State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Nanning 530005, China; 2 Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Laboratory / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China; 3 Guangxi Forestry Research Institute, Nanning 530002, China
  • Received:2012-11-12 Revised:2013-01-15 Online:2013-06-12 Published:2013-02-19
  • Contact: 杨丽涛, E-mail: liyr@gxu.edu.cn, Tel: 0771-3236407; 李杨瑞, E-mail: liyr@gxaas.net, Tel: 0771-3247689

Abstract:

Ratoon stunting disease (RSD) is one of the major diseases, which is harmful to sugarcane in the world. To investigate the molecular mechanism of gene differential expression in sugarcane under ratoon stunting disease stress, we inoculated the stem of sugarcane variety ROC22 with cane juice with RSD pathogen Leifsonia xyli subsp. xyli to induce resistance, then took stem samples at 2, 4, 6, 8, and 10 days, respectively, after inoculation, and established different cDNA mixture pools transcribed from the stem RNA mixture pools of RSD treatment and control for cDNA-SCoT analysis to detect the gene differential expressions. More than 500 bands with length of 100–1800 bp were obtained using 80 SCoT primers. A total of 30 differentially expressed EST sequences were screened out, and 22 non-redundant ESTs with high quality were obtained by cluster analyses of the ESTs sequencing. The results of BlastN showed that the differentially expressed genes of ROC22 mainly related to energy metabolism, disease defense, signal transduction, and protein metabolism. Further analysis of gene function indicated that brassinosteroid biosynthesis-like proteins, NBS-LRR type disease resistance protein, jasmonic acid induced protein, α-tubulin, abscisic stress ripening protein, proline-rich protein, and translation initiation factor eif-2b α subunit may be involved in the process of the incompatible interaction between plants and Leifsonia xyli subsp. xyli.

Key words: Sugarcane, Ratoon stunting disease, cDNA-SCoT, Differential genes

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