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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1501-1505.doi: 10.3724/SP.J.1006.2014.01501

• RESEARCH NOTES • Previous Articles     Next Articles

Differential Expression of ATP Synthesis Related Gene in Fertility Conversion of Wheat BNS Male Sterile Line

WANG Zhen,FAN Xiao-Jing,ZHANG Miao,LI Xue-Yin,ZHANG Fang-Ning,LI Gui-Dong,SHEN Fang-Di,MA Ling-Jian*   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2014-01-21 Revised:2014-06-16 Online:2014-08-12 Published:2014-06-10
  • Contact: 马翎健, E-mail: mlingjian@126.com, Tel: 13891836986

Abstract:

BNS male sterile line is a temperature-sensitive type in wheat. This study aimed at disclosing the role of ATPase α submit and the adenine phosphoribosyl transferase (APRT) in BNS male sterility. At four key stages of pollen development, the differential expressions of ATPase α submit gene and APRT were analyzed using fluorescence real-time quantitative PCR (qRT-PCR) technique. Under sterile condition, the relative expression of ATPase α submit gene decreased constantly from tetrad phase to binucleated stage. Particularly, the expression of ATPase α submit gene in uninucleated period was significantly lower in sterile plant than in fertile plant. At four stages, APRT1 had lower expressions under sterile condition than under fertile condition, whereas, APRT2 maintained low expression level in fertility conversion. The transcription levels of APRT genes increased significantly in trinucleate period, and the variation was more obvious in fertile plants than in sterile plants. Therefore, ATPase α submit is positively related to fertility conversion in BNS sterile line and APRT genes probably play a role in trinucleate period.

Key words: Wheat, BNS male sterility, ATPase &alpha, submit, Adenine phosphoribosyl transferase, qRT-PCR.

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