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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2176-2182.doi: 10.3724/SP.J.1006.2014.02176

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Constitution and Spatiotemporal Expression of Starch Branching Enzyme in Developing Wheat Grain

LIU Zheng-Shuai1,LIU Gui-Fen1,YANG Ming-Yu2,JIA Xiao1,LI Yun-Xiang1,ZHAO Fa-Mao1,*   

  1. 1 College of Biology and Enology, Taishan University, Tai’an 271021, China; 2 Tai’an No.1 Middle School, Tai’an 271000, China
  • Received:2014-04-17 Revised:2014-09-16 Online:2014-12-12 Published:2014-09-26
  • Contact: 赵法茂, E-mail: fmzhao8828@163.com

Abstract:

This study aimed at disclosing the enzymatic mechanism in amylopectin synthesis in wheat (Triticum aestivum L.). The isozyme forms, organ localization, spatiotemporal expression profile and subunits constitution of starch branching enzyme (SBE) were identified in eight wheat cultivars from different provenances using native polyacrylamide gel electrophoresis (Native-PAGE) and SDS-PAGE. Four SBE isozymes were detected in wheat endosperm, in which isozymes B and SBEIIa were localized in endosperm and leaf, whereas isozymes A and Di were exclusively present in endosperm. In the process of grain filling, Di and SBEIIa expressed first, followed by isozyme B, and isozyme A expressed finally. However, B and SBEIIa terminated to express at late filling stage. All SBE isozymes were composed of one subunit of 86–92 kD, and their spatial localization exhibited organ specificity. According to the expression level, Di, B, and SBEIIa are considered as dominant isozymes for grain endosperm development. They probably determinate the total SBE activity and serve as key factors in amylpectin biosynthesis in wheat grain and leaf.

Key words: Triticum aestivum L., Starch branching enzyme, Amylpectin, Spatiotemporal expression, Organ localization

[1]Baga M, Glaze S, Mallard C S, Chibbar R. A starch branching enzyme gene in wheat produces alternatively spliced transcripts. Plant Mol Biol, 1999, 40: 1019–1030



[2]姚大年, 李保云, 朱金宝, 梁荣奇, 刘广田. 小麦品种主要淀粉性状及面条品质预测指标的研究. 中国农业科学, 1999, 32(6): 84–88



Yao D N, Li B Y, Zhu J B, Liang R Q, Liu G T. Study on main starch properties and predictive indexes of noodle quality in common wheat. Sci Agric Sin, 1999, 32(6): 84–88 (in Chinese with English abstract)



[3]McCormick K M, Panozzo J F, Hong S H. A swelling power test for selecting potential noodle quality wheats. Aust J Agric Res, 1991, 42: 317–323.



[4]Myers A M, Morell M K, JamesM G, Ball S G. Recent progress toward understanding the amylopectin crystal. Plant Physiol, 2000, 122: 989–997



[5]Kossmann J, Lloyd J. Understanding and influencing starch biochemistry. Crit Rev Plant Sci, 2000, 19: 171–226



[6]James M G, Denyer K, Myers A M. Starch synthesis in the cereal endosperm. Curr Opin Plant Biol, 2003, 6: 215–222



[7]Jeon J S, Ryoo N, Hahn T R, Walia H, Nakamura Y. Starch biosynthesis in cereal endosperm. Plant Physiol Biochem, 2010, 48: 383–392



[8]Guan H P, Preiss J. Differentiation of the properties of the branching isozymes from maize (Zea mays). Plant Physiol, 1993, 102: 269–1273



[9]Takeda Y, Guan H-P, Preiss J. Branching of amylose by the branching isoenzymes of maize endosperm. Carbohydr Res, 1993, 240: 253–263



[10]Morell M K, Blennow A, Hashemi B K, Samuel M S. Differential expression and properties of starch branching nzyme isoforms in developing wheat endosperm. Plant Physiol, 1997, 113: 201–208



[11]Regina A, Kosar H B, Li Z, Pedler A, Mukai Y, Yamamoto M, Gale K, Sharp P J, Morell M K, Rahman S. Starch branching enzyme IIb in wheat is expressed at low levels in the endosperm compared to other cereals and encoded at a non-syntenic locus. Planta, 2005, 222: 899–909



[12]Schwall G P, Safford R, Westcott R J, Jeffcoat R J, Tayal A, Shi Y C, Gidley M G, Jobling S A. Production of very-high-amylose potato starch by inhibition of SBE A and SBE B. Nation Biotechnol, 2000, 18: 551–554



[13]Nishi A, Nakamura Y, Tanaka N, Satoh H. Biochemical and genetic effects of amylose–extender mutation in rice endosperm. Plant physiol, 2001, 127: 459–472



[14]Regina A, Bird D, Topping D, Bowden S, Freeman J, Barsby T, Hashemi B K, Li Z, Rahman S, Morell M. High amylose wheat generated by RNA-interference improves indices of large bowel health in rats. Proc Natl Acad Sci USA, 2006, 103: 3546–3551.



[15]Yamanouchi H, Nakamura Y. Organ specificity of isoforms of starch branching enzyme (Q-enzyme) in rice. Plant Cell Physiol, 1992, 33: 985–991



[16]李太贵, 沈波, 陈能, 罗玉坤. Q酶对水稻籽粒垩白形成的影响. 作物学报, 1997, 23: 338–344



Li T G, Shen B, Cheng N, Luo Y K. Effect of Q-enzymeon the chalkiness formation of rice grain. Acta Agron Sin, 1997, 23: 338–344 (in Chinese with English abstract)



[17]Nagamine T, Yoshida H, Komae K. Varietal differences and chromosome locations of multiple isoforms of starch branching enzyme in wheat endosperm. Phytochemistry, 1997, 46: 23–26



[18]Davis B J. Disc electrophoresis II Method and application of human serum proteins. Ann NY Acad Sci, 1964, 121: 404–427



[19]Hager D A, Burgess R R. Elution of proteins from SDS-polyacrylamide gels, removal of SDS, and renaturation of enzymatic activity: Results with sigma subunit of E. coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem, 1980, 109: 76–86



[20]Dynan W S, Jendrisak JJ, Hager D A, Burgess R D. Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme). J Biol Chem, 1981, 256: 5860–5865



[21]Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685



[22]Hzard B, Zhang X Q, Colasuonno P, Uauy C, Beckles D M, Dubcovsky J. Induced mutations in the starch branching enzyme II (SBEII) genes increase amylase and resistant starch content in durum wheat. Crop Sci, 2012, 52: 1754–1766



[23]Carciofi M, Blennow A, Jensen S L, Shaik S S, Henriksen A, Buleon A, Holm P B, Hebelstrup K. Concerted suppression of all starch branching enzyme genes in barley produces amylase-only starch granules. BMC Plant Biol, 2012, 12: 223–239



[24]Rahman S, Hashemi B K, Samuel M S, Hill A, Abbott D C, Skerritt J H, Preiss J, Appels R, Morell M K. The major proteins of wheat endosperm starch granules. Aust J Plant Physiol, 1995, 22: 793–803



[25]Tetlow I J, Wait R, Lu Z X, Akkasaeng R, Bowsher C G, Esposito S, Hashemi B K, Morell M K, Emes M J. Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions. Plant Cell, 2004, 16: 694–708



[26]Tetlow I J, Beisel K G, Cameron S, Makhmoudova, Liu F, Bresolin N S, Wait R, Morell M K, Emes M J. Analysis of protein complexes in wheat amyloplasts reveals functional interactions among starch biosynthetic enzymes. Plant Physiol, 2008, 146: 1878–1891



[27]Hennen-Bierwagen T A, Liu F, Marsh R S, Kim S, Gan Q, Tetlow I J, Emes M J, James M G, Myers A M. Starch biosynthetic enzymes from developing maize endosperm associate in multisubunit complexes. Plant Physiol, 2008, 146: 1892–1908

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