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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 389-398.doi: 10.3724/SP.J.1006.2017.00389

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Expression Analysis of Microtubule Binding Protein CLASP Family Genes in Gossypium hirsutum L.

ZHU Shou-Hong,ZHAO Lan-Jie,LIU Yong-Chang*,LI Yan-Jun,ZHANG Xin-Yu,SUN Jie   

  1. College of Agronomy, Shihezi University / Key Oasis Eco-agriculture Laboratory of Production and Construction Group, Shihezi 832003, China
  • Received:2016-04-04 Revised:2016-09-18 Online:2017-03-12 Published:2016-10-09
  • Contact: 刘永昌, E-mail: liuyongchang2003@126.com, Tel: 18799292449 E-mail:zhushouhong2014@126.com
  • Supported by:

    This study was support by the National Natural Science Foundation (U1128301), the Doctor Funds of Xinjiang Crops (2013BB003), and the Startup Project of Advanced Talents of Shihezi University (RCZX201218).

Abstract:

CLIP-Associated Proteins (CLASPs) are microtubule associated proteins (MAPS) which are regulators of microtubule structure and function, and play a key role in plant growth and morphogenesis. In this study, six CLASP genes were preliminarily identified in Gossypium hirsutum L. by bioinformatics. Based on multiple sequence alignment and phylogenetic tree analysis, we divided the six CLASP genes into I and II subfamilies. The subfamily I has HEAT domain and CLASP-N structure; and the subfamily II only has CLASP-N structure. Real-time quantitative reverse transcription PCR (qRT-PCR) revealed that GhCLASP family genes expressed in root, stem, leaf, petal and fiber at different developmental periods,and there existed different expression patterns. CotAD_63740 (GenBank accession No. KX881965) and CotAD_04861 (GenBank accession No. KX881961) were preferentially expressed in fiber; CotAD_48232 (GenBank accession No. KX881962), CotAD_48665 (GenBank accession No. KX881963), CotAD_55570 (GenBank accession No. KX881964), and CotAD_68468 (GenBank accession No. KX881966) were preferentially expressed in stem. Different expression patterns suggest that they may play different roles during cotton development. The results will be helpful for the further analysis of CLASP family proteins in cotton.

Key words: Gossypium hirsutum L, Microtubule binding protein, Microtubule, Expression analysis

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