Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (11): 1621-1630.doi: 10.3724/SP.J.1006.2018.01621

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Mapping of sdb1 Mutant with a Semi-dwarfism and Bigger Seed in Rice

Yi-Ran TAO,Yu-Zhen XIONG,Jia XIE,Wei-Jiang TIAN,Xiao-Qiong ZHANG,Xiao-Bo ZHANG,Qian ZHOU,Xian-Chun SANG,Xiao-Wen WANG()   

  1. Basic Experiment Teaching Center of Agronomy / College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2018-03-09 Accepted:2018-07-20 Online:2018-11-12 Published:2018-08-07
  • Contact: Xiao-Wen WANG E-mail:xwwang78@126.com
  • Supported by:
    This study was supported by the Chongqing Research Program of Basic Research and Frontier Technology(cstc2015jcyjA80008);the Project of Chongqing Science & Technology Commission Grants(cstc2016shms- ztzx8007);the Project of Chongqing Science & Technology Commission Grants(cstc2017shms-xdny80057);the College Students’ Innovation and Entrepreneurship Training Scientific Research Project(201710635043)

RichHTML338

50

PDF

1054

PDF

69

Abstract:

Moderate dwarfing is conducive to improving the lodging resistance of rice and further affecting its yield and quality, which is one of the important alternatives in rice breeding. Therefore, it is of great significance to study the molecular mechanism of shortened stem formation. In order to identify new dwarf resources and to explore the molecular regulation mechanism of plant height formation, a semi-dwarf and bigger seed (sdb1) mutant was identified from the progeny of indica restorer line Jinhui 10 with seed treated by ethyl methane sulfonate (EMS). This paper performed systematic studies in phenotypic identification, cytological observation, genetic analysis and gene mapping. At maturity stage, the plant height of sdb1 was only 76.66 cm, significantly shorter than 117.43 cm of wild type, resulting in a 34.72% decrease. It was found from the paraffin sections of the stem that the mutant had no significant change in stem cell length compared with the wild-type cells, it reduced cell width caused significantly smaller cell size, and the number of lateral cells significantly increased. The decrease of longitudinal cells should be the major cause of sdb1 semi-dwarfism. In addition to reduced plant height of sdb1, another typical mutational trait was the larger grain size, the 1000-grain weight increased from wild-type’s 24.83 g to mutant’s 29.00 g, reaching an extremely significant difference. The number of parenchyma cells increased from 666.30 in WT to 813.21 in sdb1, with an increase of 22.05%. The increase of cell number resulted in a significant increase of grain length, width and thickness. The mutant also had increased number of sdb1 mesophyll cells, resulting in significantly higher photosynthetic pigment content than wild type, and dark green leaves. Genetic analysis showed that a recessive nuclear gene regulated the mutant phenotype. Based on the F2 recessive plants of Zhonghua 11/sdb1, the gene was finally mapped between markers RM16632 and J50-7 on chromosome 4, with a physical distance of 406 kb. This research lays a foundation for gene cloning and function research, and is conducive to further understanding the genetic model of rice plant height, which has the potential value for agricultural production research.

Key words: rice (Oryza sativa L.), dwarfism, bigger seeds, gene mapping, sdb1

Table 1

Primer sequences used for gene mapping"

引物名称
Primer name		 上游引物序列
Upstream primer sequence (5'-3')		 下游引物序列
Downstream primer sequence (5'-3')
J50-3 TGCCTCCTTGTGTGTGGAGT TCCAAGCACTGACTTCATAGC
J50-2 CCACCAGTGATCCCTCATACA TCCGAGCGATTCTACTGGTC
J50-7 CCTCGTGTGGAATCTCGTTC GTAACAGTAGGTGCGAAGATGG
J50-53 GCGTTGTGTGCTCACTGG CCACTTGTCAGGTCTCCATC
J50-65 CCGGACGAGCCAATCAG CGGTGGCTTCTCCCAAGG

Fig. 1

Plant phenotype detection of wild type (WT) and sdb1 mutant A: phenotype of the wild type and the sdb1 at the seedling stage, Bar=5 cm; B: phenotype of the wild type and the sdb1 at the heading stage, Bar=10 cm; C: phenotype of the wild type and the sdb1 at the filling stage, Bar=15 cm; D: internodes of the wild type and the sdb1 at the filling stage, Bar=5 cm; E: panicle of the wild type and the sdb1 at the filling stage, Bar=5 cm; F: statistical analysis of panicle and internodes length of the wild type and the sdb1. **Significantly different at P<0.01."

Fig. 2

Stems paraffin sections of the wild type (WT) and sdb1 mutantA and B: longitudinal section of internodes of the wild type and the sdb1, Bar=100 μm; C: enlarged views of the red box in A, Bar=100 μm; D: enlarged views of the red box in B, Bar=100 μm; E:statistics of the cells number of the wild type and the sdb1 in the same area of longitudinal section; F: comparison of the cells size of the wild type and the sdb1 in longitudinal section; G and H: statistics of the cell length and width of the wild type and the sdb1in longitudinal section; I and J: gross section of internodes of the wild type and the sdb1, Bar=100 μm; K: enlarged views of the red box in I, Bar=25 μm; L: enlarged views of the red box in J, Bar=25 μm; M: enlarged views of the black box in I, Bar=50 μm; N: enlarged views of the black box in J, Bar=50 μm; O: statistics of the cells number of the wild type and the sdb1 in the same area of cross section; P:comparison of the cells size of the wild type and the sdb1 in cross section. **Significantly different at P < 0.01."

Fig. 3

Comparison of the grains between the wild-type and the sdb1 mutant A: grains of WT and the sdb1, Bar=0.5 cm; B and C: cross section of the grains of WT and the sdb1, Bar=500 μm; D: enlarged views of the red box in B, Bar=50 μm; E: enlarged views of the red box in C, Bar=50 μm; F: statistical chart of 1000-grain weight of WT and the sdb1; G and H: statistical chart of cell length (G), width (H) and thickness (H) of WT and the sdb1; I: statistical the number of parenchyma cells of WT (B) and the sdb1 (C). **Significantly different at P < 0.01."

Fig. 4

Frozen sections and photosynthetic pigment contents at flowering stage A: the middle of flag leaf of the wild type (WT) and the mutant sdb1 at heading stage; B and C: frozen section of crosscutting leaf of the wild type and the mutant sdb1, Bar=50 μm; D: chlorophyll content of the flag leaf blade; E:chlorophyll content of the second leaf from the top; F: chlorophyll content of the third leaf from the top; Chl a: chlorophyll a; Chl b: chlorophyll b; Car: carotenoid. **Significantly different at P<0.01; *Significantly different at P<0.05."

Fig. 5

Molecular mapping of SDB1 gene on rice chromosome 4"

Table 2

RGAP annotated genes encoding expressed and functional proteins in the restricted region of SDB1 locus"

基因名称
Locus name		 基因注释
Gene annotation
LOC_Os04g23170 Expressed protein
LOC_Os04g23180 Cation efflux family protein, putative, expressed
LOC_Os04g23200 Expressed protein
LOC_Os04g23210 Expressed protein
LOC_Os04g23220 Expressed protein
LOC_Os04g23230 Expressed protein
LOC_Os04g23319 Expressed protein
LOC_Os04g23330 Expressed protein
LOC_Os04g23420 Expressed protein
LOC_Os04g23440 Helix-loop-helix DNA-binding domain containing protein, expressed
LOC_Os04g23460 Expressed protein
LOC_Os04g23550 Basic helix-loop-helix family protein
LOC_Os04g23580 Xylosyltransferase, putative, expressed
LOC_Os04g23600 D-mannose binding lectin family protein, expressed
LOC_Os04g23610 Expressed protein
LOC_Os04g23620 D-mannose binding lectin family protein, expressed
LOC_Os04g23630 Expressed protein
[1] Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush G S, Kitano H, Matsuoka M . Green revolution: a mutant gibberellin- synthesis gene in rice. Nature, 2002,416:701-702
doi: 10.1038/416701a
[2] Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y . Positional cloning of rice semidwarfing gene, sd-1: rice ‘‘green revolution gene’’ encodes a mutant enzyme involved in gibberellin synthesis. DNA Res, 2002,9:11-17
[3] Spielmeyer W, Ellis M H, Chandler P M . Semidwarf (sd-1 ) ‘‘green revolution’’ rice, contains a defective gibberellin 20-oxidase gene.Proc Natl Acad Sci USA, 2002,99:9043-9048
[4] Luo D, Xu H, Liu Z, Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R, Liu Y G . A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet, 2013,45:573-577
doi: 10.1038/ng.2570 pmid: 23502780
[5] Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G, Dong G, Zeng D, Lu Z, Zhu X, Qian Q, Li J . Regulation ofOsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet, 2010,42:541-544
doi: 10.1038/ng.591 pmid: 20495565
[6] Miura K, Ikeda M, Matsubara A, Song X J, Ito M, Asano K, Matsuoka M, Kitano H, Ashikari M . OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat Genet, 2010,42:545-549
doi: 10.1038/ng.592 pmid: 20495564
[7] 王翠红, 马建, 王帅, 田鹏, 岂长燕, 赵志超, 王久林, 王洁, 程治军, 张欣, 郭秀平, 雷财林 . 一个新的水稻D1基因等位突变体的遗传鉴定与基因功能分析. 作物学报, 2016,42:1261-1272
doi: 10.3724/SP.J.1006.2016.01261
Wang C H, Ma J, Wang S, Tian P, Yi C Y, Zhao Z C, Wang J L, Wang J, Cheng Z J, Zhang X, Guo X P, Lie C L . Genetic identification and gene function analysis of a new allelic mutant of D1 gene in rice. Acta Agron Sin, 2016,42:1261-1272 (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2016.01261
[8] Zhang Z, Zhang Y, Tan H, Wang Y, Li G, Liang W, Yuan Z, Hu J, Ren H, Zhang D . Rice morphology determinant encodes the type II formin FH5 and regulates rice morphogenesis. Plant Cell, 2011,23:681-700
doi: 10.1105/tpc.110.081349
[9] Yang W B, Ren S L, Zhang X M, Gao M J, Ye S H, Qi Y B, Zheng Y Y, Wang J, Zeng L J, Li Q, Huang S J, He Z H . Bent uppermost internode1 encodes the class II formin FH5 crucial for actin organization and rice development. Plant Cell, 2011,23:661-680
doi: 10.1105/tpc.110.081802
[10] Li G, Liang W Q, Zhang X Q, Ren H Y, Hu J P, Bennett M J, Zhang D B . Rice actin-binding protein RMD is a key link in the auxin-actin regulatory loop that controls cell growth. Proc Natl Acad Sci USA, 2014,111:10377-10382
doi: 10.1073/pnas.1401680111
[11] 牛静, 陈赛华, 赵婕妤, 曾召琼, 蔡茂红, 周亮, 刘喜, 江玲, 万建民 . 水稻株型突变体rad-1和rad-2的鉴定与功能基因克隆. 作物学报, 2015,41:1621-1631
Niu J, Chen S H, Zhao J Y, Zeng Z Q, Cai M H, Zhou L, Liu X, Jiang L, Wan J M . Identification and map-based cloning of rad-1 and rad-2, two rice architecture determinant mutants. Acta Agron Sin, 2015,41:1621-1631 (in Chinese with English abstract)
[12] Hu J, Zhu L, Zeng D, Gao Z, Guo L, Fang Y, Zhang G, Dong G, Yan M, Liu J, Qian Q . Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol, 2010,73:283-292
[13] Luan W J, Liu Y Q, Zhang F X, Song Y L, Wang Z Y, Peng Y K, Sun Z X . OsCD1 encodes a putative member of the cellulose synthase-like D sub-family and is essential for rice plant architecture and growth. Plant Biotechnol J, 2011,9:513-524
[14] Sunohara H, Kawai T, Shimizu-Sato S, Sato Y, Sato K, Kitano H . A dominant mutation ofTWISTED DWARF 1 encoding an α-tubulin protein causes severe dwarfism and right helical growth in rice. Genes Genet Syst, 2009,84:209-218
[15] Segami S, Kono I, Ando T, Yano M, Kitano H, Miura K, Iwasaki Y . Small and round seed 5 gene encodes alpha-tubulin regulating seed cell elongation in rice. Rice, 2012,5:4
[16] 李自壮, 徐乾坤, 余海平, 周亭亭, 薛大伟, 曾大力, 郭龙彪, 钱前, 任德勇 . 水稻淡黄叶矮化突变体yld的遗传分析及基因定位. 作物学报, 2017,43:522-529
doi: 10.3724/SP.J.1006.2017.00522
Li Z Z, Xu Q K, Yu H P, Zhou T T, Xue D W, Zeng D L, Guo L B, Qian Q, Ren D Y . Genetic analysis and gene mapping of yellow leaf and dwarf ( yld) mutant in rice. Acta Agron Sin, 2017,43:522-529 (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2017.00522
[17] 张玲, 郭爽, 汪玲, 张天泉, 庄慧, 龙珏臣, 何光华, 李云峰 . 水稻矮化并花发育异常突变体dwarf and deformed flower 2 (ddf2)的基因定位与候选基因分析. 中国农业科学, 2015,48:1873-1881
Zhang L, Guo S, Wang L, Zhang T Q, Zhuang H, Long Y C, He G H, Li Y F . Gene mapping and candidate gene analysis of a dwarf and deformed flower 2 (ddf2) mutant in rice( Oryza sativa). Sci Agric Sin, 2015,48:1873-1881 (in Chinese with English abstract)
[18] 王文乐, 冯丹, 吴金霞, 张治国, 路铁刚 . 水稻矮化基因WLD1的图位克隆. 植物学报, 2017,52:54-60
doi: 10.11983/CBB16010
Wang W L, Feng D, Wu J X, Zhang Z G, Lu T G . Gene mapping of a dwarf gene WLD1 in rice. Chin Bull Bot, 2017,52:54-60 (in Chinese with English abstract)
doi: 10.11983/CBB16010
[19] 王晓雯, 蒋钰东, 廖红香, 杨波, 邹帅宇, 朱小燕, 何光华, 桑贤春 . 水稻白穗突变体wp4的鉴定与基因精细定位. 作物学报, 2015,41:838-844
Wang X W, Jiang Y D, Liao H X, Yang B, Zou S Y, Zhu X Y, He G H, Sang X C . Identification and fine gene mapping of rice white ear mutant wp4. Acta Agron Sin, 2015,41:838-844 (in Chinese with English abstract)
[20] Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y . A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell, 2005,17:776-790
[21] Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuok M . A rice brassinosteroid-deficient mutant, ebisu dwarf (d2 ), is caused by a Loss of function of a new member of cytochrome P450. Plant Cell, 2003,15:2900-2910
[22] Liu J M, Park S J, Huang J, Lee E J, Xuan Y H, Je B I, Kumar V, Priatama R A, Raj K V, Kim S H, Min M K, Cho J H, Kim T H, Chandran A K, Jung K H, Takatsuto S, Fujioka S, Han C D . Loose plant architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22- hydroxylated and 6-deoxo brassinosteroids in rice. J Exp Bot, 2016,67:1883-1895
doi: 10.1093/jxb/erw002
[23] Duan Y, Li S, Chen Z , Zheng L, Diao Z, Zhou Y, Lan T, Guan H, Pan R, Xue Y, Wu W. Dwarf and Deformed Flower 1, encoding an F-box protein, is critical for vegetative and floral development in rice( Oryza sativa L.). Plant J, 2012,72:829-842
[24] Li J, Jiang J, Qian Q, Xu Y, Zhang C, Xiao J, Du C, Luo W, Zou G, Chen M, Huang Y, Feng Y, Cheng Z, Yuan M, Chong K . Mutation of rice BC12/GDD1, Which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation. Plant Cell, 2011,23:628-640
[25] 张孝波, 谢佳, 张晓琼, 田维江, 何沛龙, 刘思岑, 何光华, 钟秉强, 桑贤春 . 水稻矮化剑叶卷曲突变体dcfl1的鉴定与基因精细定位. 中国农业科学, 2017,50:1551-1558
doi: 10.3864/j.issn.0578-1752.2017.09.001
Zhang X B, Xie J, Zhang X Q, Tian W J, He P L, Liu S C, He G H, Zhong B Q, Sang X C . Identification and gene fine mapping of curling mutant dcfl1 in dwarf flag leaf. Sci Agric Sin, 2017,50:1551-1558 (in Chinese with English abstract)
doi: 10.3864/j.issn.0578-1752.2017.09.001
[26] Sun L, Li X, Fu Y, Zhu Z, Tan L, Liu F, Sun X, Sun X, Sun C . GS6, a member of the GRAS gene family, negatively regulates grain size in rice. J Integr Plant Biol, 2013,55:938-949
doi: 10.1111/jipb.12062 pmid: 23650998
[27] Tong H, Jin Y, Liu W, Li F, Fang J, Yin Y, Qian Q, Zhu L, Chu C . DWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice. Plant J, 2009,58:803-816
[28] Tong H, Liu L, Jin Y, Du L, Yin Y, Qian Q, Zhu L, Chu C . Dwarf and low-tillering acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice. Plant Cell, 2012,24:2562-2577
doi: 10.1105/tpc.112.097394
[29] Liu J, Chen J, Zheng X, Wu F, Lin Q, Heng Y, Tian P, Cheng Z, Yu X, Zhou K, Zhang X, Guo X, Wang J, Wang H, Wan J . GW5 acts in the brassinosteroid signalling pathway to regulate grain width and weight in rice. Nat Plants, 2017,3:17043
doi: 10.1038/nplants.2017.43 pmid: 28394310
[30] Hirano K, Yoshida H, Aya K, Kawamura M, Hayashi M, Hobo T, Sato-Izawa K, Kitano H, Ueguchi-Tanaka M, Matsuoka M . SMALL ORGAN SIZE 1 and SMALL ORGAN SIZE 2/ DWARF AND LOW-TILLERING form a complex to integrate auxin and brassinosteroid signaling in rice. Mol Plant, 2017,10:590-604
[31] Qiao S, Sun S, Wang L, Wu Z, Li C, Li X, Wang T, Leng L, Tian W, Lu T, Wang X . The RLA1/SMOS1 transcription factor functions with OsBZR1 to regulate brassinosteroid signaling and rice architecture. Plant Cell, 2017,29:292-309
[32] Zhang G H, Li S Y, Wang L, Ye W J, Zeng D L, Rao Y C, Peng Y L, Hu J, Yang Y L, Xu J, Ren D Y, Gao Z Y, Zhu L, Dong G J, Hu X M, Yan M X, Guo L B, Li C Y, Qian Q . LSCHL4 from japonica cultivar, which is allelic to NAL1, increases yield of indica super rice 93-11. Mol Plant, 2014,7:1350-1364
[33] Zou J, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L . The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. Plant J, 2006,48:687-698
[34] 赵祥强, 梁国华, 周劲松, 严长杰, 曹小迎, 顾铭洪 . 矮泰引-3中半矮秆基因的分子定位. 遗传学报, 2005,32:189-196
Zhao X Q, Liang G H, Zhou J S, Yan C J, Cao X Y, Gu M H . Molecular mapping of semi-dwarf gene in Datiai-3. J Genet Genomics, 2005,32:189-196 (in Chinese with English abstract)
[35] 高振宇, 刘晓辉, 郭龙彪, 刘坚, 董国军, 胡江, 韩斌, 钱前 . 一新的水稻多蘖矮秆突变体tddl(t)的分离及基因的精细定位. 科学通报, 2009,54:1238-1243
Gao Z Y, Liu X H, Guo L B, Liu J, Dong G J, Hu J, Han B, Qian Q . Isolation and fine gene mapping of a new rice dwarf mutant tddl(t). Chin Sci Bull, 2009,54:1238-1243 (in Chinese with English abstract)
[36] Heang D, Sassa H . An atypical bHLH protein encoded by POSITIVE REGULATOR OF GRAIN LENGTH 2 is involved in controlling grain length and weight of rice through interaction with a typical bHLH protein APG. Breed Sci, 2012,62:133-141
[37] Miyamoto K, Shimizu T, Mochizuki S, Nishizawa Y, Minami E, Nojiri H, Yamane H, Okada K . Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice. Protoplasma, 2013,250:241-249
[38] Funabiki A, Takano S, Matsuda S, Tokuji Y, Takamure I, Kato K . The rice REDUCED CULM NUMBER11 gene controls vegetative growth under low-temperature conditions in paddy fields independent of RCN1/OsABCG5 .Plant Sci, 2013,211:70-76
[39] Takano S, Matsuda S, Funabiki A, Furukawa J, Yamauchi T, Tokuji Y, Nakazono M, Shinohara Y, Takamure I, Kato K . The riceRCN11 gene encodes β1,2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones. Plant Sci, 2015,236:75-88
[40] Ueno D, Sasaki A, Yamaji N, Miyaji T, Fujii Y, Takemoto Y, Moriyama S, Che J, Moriyama Y, Iwasaki K, Ma J F . A polarly localized transporter for efficient manganese uptake in rice. Nat Plants, 2015,1:15170
doi: 10.1038/nplants.2015.170 pmid: 27251715
[41] Zhang M, Liu B . Identification of a rice metal tolerance protein OsMTP11 as a manganese transporter. PLoS One, 2017,12:e0174987
doi: 10.1371/journal.pone.0174987
[42] Passricha N, Saifi S, Ansari M W, Tuteja N . Prediction and validation of cis -regulatory elements in 5′ upstream regulatory regions of lectin receptor-like kinase gene family in rice. Protoplasma, 2017,254:669-684
[1] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[2] YANG Si-Jie, DU Qi-Di, CHAI Shou-Xi, XIONG Hong-Chun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, GUO Hui-Jun, LIU Lu-Xiang. Genetic mapping of mutant genes on flag leaf length and width in wheat [J]. Acta Agronomica Sinica, 2025, 51(6): 1548-1557.
[3] YUAN Xin, ZHAO Zhuo-Fan, ZHAO Rui-Qing, LIU Xiao-Wei, ZHENG Ming-Min, LIU Yu-Sheng, DONG Hao-Sheng, DENG Li-Juan, CAO Mo-Ju, HUANG Qiang. Genetic analysis and molecular identification of a small kernel mutant mn-like1 in maize [J]. Acta Agronomica Sinica, 2025, 51(6): 1569-1581.
[4] JIA Shu-Han, HE Can, CHEN Min, LIU Jia-Xin, HU Wei-Min, HU Jin, GUAN Ya-Jing. Study on the quality differences of seeds with different pre-harvest sprouting levels and the grading of pre-harvest sprouting in hybrid rice [J]. Acta Agronomica Sinica, 2024, 50(9): 2310-2322.
[5] SU Shuai, LIU Xiao-Wei, NIU Qun-Kai, SHI Zi-Wen, HOU Yu-Wei, FENG Kai-Jie, RONG Ting-Zhao, CAO Mo-Ju. Identification and gene cloning of leafy dwarf mutant lyd1 in maize [J]. Acta Agronomica Sinica, 2024, 50(5): 1124-1135.
[6] WAN Ying-Chun, BAN Yi-Jie, JIANG Yu-Dong, WANG Ya-Xin, LIU Jing-Jing, LIU Xiao-Qing, CHENG Yu-Lin, WANG Nan, FENG Ping. Phenotypic identification and fine mapping of male sterile mutant tpa1 in rice [J]. Acta Agronomica Sinica, 2024, 50(5): 1104-1114.
[7] YU Yao, WANG Zi-Yao, ZHOU Si-Rui, LIU Peng-Cheng, YE Ya-Feng, MA Bo-Jun, LIU Bin-Mei, CHEN Xi-Feng. Phenotypic identification and disease resistance mechanism analysis of rice lesion mutant lms1 [J]. Acta Agronomica Sinica, 2024, 50(4): 857-870.
[8] JIA Lu-Qi, SUN You, TIAN Ran, ZHANG Xue-Fei, DAI Yong-Dong, CUI Zhi-Bo, LI Yang-Yang, FENG Xin-Yu, SANG Xian-Chun, and WANG Xiao-Wen. Identification of the rgs1 mutant with rapid germination of seed and isolation of the regulated gene in rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2288-2295.
[9] TANG Jie, LONG Tuan, WU Chun-Yu, LI Xin-Peng, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jin. Identification of OsGMS2 and construction of seed production system for genic male sterile line in rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2025-2038.
[10] WANG Xing-Rong, ZHANG Yan-Jun, TU Qi-Qi, GONG Dian-Ming, QIU Fa-Zhan. Identification and gene localization of a novel maize nuclear male sterility mutant ms6 [J]. Acta Agronomica Sinica, 2023, 49(8): 2077-2087.
[11] LIN Xiao-Xin, HUANG Ming-Jiang, WEI Yi, ZHU Hong-Hui, WANG Zi-Yi, LI Zhong-Cheng, ZHUANG Hui, LI Yan-Xi, LI Yun-Feng, CHEN Rui. Identification and gene mapping of long grain and degenerated palea (lgdp) in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1699-1707.
[12] DING Jie-Rong, MA Ya-Mei, PAN Fa-Zhi, JIANG Li-Qun, HUANG Wen-Jie, SUN Bing-Rui, ZHANG Jing, LYU Shu-Wei, MAO Xing-Xue, YU Hang, LI Chen, LIU Qing. Ubiquitin receptor protein OsDSK2b plays a negative role in rice leaf blast resistance and osmotic stress tolerance [J]. Acta Agronomica Sinica, 2023, 49(6): 1466-1479.
[13] DAI Wen-Hui, ZHU Qi, ZHANG Xiao-Fang, LYU Shen-Yang, XIANG Xian-Bo, MA Tao, CHEN Yu-Jie, ZHU Shi-Hua, DING Wo-Na. Identification and gene mapping of brittle culm mutant bc21 in rice [J]. Acta Agronomica Sinica, 2023, 49(5): 1426-1431.
[14] YAN Xin, XIANG Chao, LIU Rong, LI Guan, LI Meng-Wei, LI Zheng-Li, ZONG Xu-Xiao, YANG Tao. Fine mapping of flower colour gene in pea (Pisum sativum L.) based on BSA-seq technique [J]. Acta Agronomica Sinica, 2023, 49(4): 1006-1015.
[15] LI Qiu-Ping, ZHANG Chun-Long, YANG Hong, WANG Tuo, LI Juan, JIN Shou-Lin, HUANG Da-Jun, LI Dan-Dan, WEN Jian-Cheng. Physiological characteristics analysis and gene mapping of a semi-sterility plant mutant sfp10 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2023, 49(3): 634-646.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd