单粒花生主要脂肪酸含量近红外预测模型的建立及其应用

doi:10.3724/SP.J.1006.2019.94016

Abstract

Abstract:

Profile of fatty acid is the main factor determining the nutritional value and shelf life of peanut. High oleate peanut has been increasingly favored by customers and peanut processing enterprises, as it provides a prolonged shelf-life and a beneficial effect on human health. Therefore, creating high oleate peanut cultivar is an important objective for peanut breeding. Developing a nondestructive method which could detect the fatty acid composition in peanut rapidly, efficiently and accurately is an important technical support for accelerating the processing. In this study, models for predicting the contents of main fatty acids (oleic acid, linoleic acid and palmitic acid) in a single peanut seed were built up using near-infrared reflectance spectroscopy (NIRS). The coefficient of multiple determination (R ²) and the root mean squared error of external calibration (RMSECV) of prediction models were 0.907 and 3.463 for oleic acid, 0.918 and 2.824 for linoleic acid, 0.824 and 0.782 for palmitic acid, respectively. One hundred peanut seeds were analyzed by both NIR and gas chromatography (GC) to validate the accuracy of the prediction models. The correlation coefficients of oleic acid, linoleic acid and palmitic acid between NIR values and GC values were 0.88, 0.90, and 0.71, respectively, suggesting that these models could accurately predict the contents of the three main fatty acids in a single peanut seed. Furthermore, based on these prediction models, a breeding method of high oleate peanut without assistance of molecular marker was developed, and a high oleate peanut cultivar Zhonghua 215 was successfully bred.

Key words: single peanut seed, fatty acid, near infrared model, high oleic acid, breeding method

Jian-Guo LI,Xiao-Meng XUE,Zhao-Hua ZHANG,Zhi-Hui WANG,Li-Ying YAN,Yu-Ning CHEN,Li-Yun WAN,Yan-Ping KANG,Dong-Xin HUAI,Hui-Fang JIANG,Yong LEI,Bo-Shou LIAO. Establishment and applicant of near-infrared reflectance spectroscopy models for predicting main fatty acid contents of single seed in peanut[J].Acta Agronomica Sinica, 2019, 45(12): 1891-1898.

Figures/Tables 10

Fig. 1

Fig. 2

Table 1

Fig. 3

Suppementary Table 1

Results from GC and NIR of oleic acid, linoleic acid and palmitic acid contents in single peanut seed"

样品 Sample	C18:1		C18:2		C16:0
样品 Sample	NIR	GC	NIR	GC	NIR	GC
1	52.9	49.9	26.0	31.9	11.1	10.4
2	80.0	74.1	1.6	9.2	6.7	6.5
3	70.3	60.0	10.1	21.6	8.5	10.5
4	73.5	69.9	8.0	14.3	7.8	7.5
5	68.5	64.6	11.4	14.3	8.5	8.1
6	67.0	49.7	13.3	29.1	8.7	8.5
7	69.2	59.9	11.5	20.8	8.5	10.3
8	64.4	39.1	14.4	35.5	9.7	13.9
9	81.2	69.7	1.3	12.3	6.1	6.4
10	83.7	69.6	-1.7	11.3	5.6	9.2
11	82.4	74.6	-0.5	9.6	5.9	7.7
12	67.8	54.9	13.5	23.5	8.7	10.6
13	54.4	49.7	24.4	29.9	11.1	9.4
14	89.5	85.4	-5.8	1.4	4.8	5.8
15	88.2	85.1	-4.9	1.2	5.1	5.1
16	57.1	44.7	22.8	32.5	10.3	15.5
17	86.2	79.8	-3.6	2.5	5.3	6.9
18	93.3	79.8	-9.2	2.4	3.9	7.0
19	55.7	38.5	23.8	37.2	10.4	15.6
20	75.0	69.6	6.4	12.8	7.1	6.1
21	91.8	79.7	-7.7	2.1	4.5	7.5
22	66.7	54.9	13.7	24.3	8.6	12.6
23	60.3	44.8	20.3	33.0	10.0	13.8
24	59.2	49.8	20.1	32.0	10.2	9.9
25	62.0	44.8	17.5	31.1	9.3	11.0
26	90.8	79.7	-6.9	2.0	5.2	6.8
27	81.1	74.8	1.1	7.0	6.3	7.6
28	68.2	59.9	12.3	21.2	9.2	8.9
29	71.8	55.0	9.6	25.8	8.2	8.8
30	77.4	69.6	4.5	11.7	7.1	6.4
31	74.7	74.2	5.9	10.6	7.7	6.8
32	85.2	84.9	-2.1	2.1	5.7	6.0
33	83.8	74.3	-1.8	8.8	6.1	5.6
34	66.1	45.0	25.3	32.3	10.5	12.9
35	71.4	59.8	9.5	20.8	8.2	7.4
36	73.3	69.8	7.6	11.9	8.1	8.0
37	61.1	54.8	18.5	26.1	10.1	8.0
38	72.2	54.9	8.5	26.1	7.7	10.6
39	90.9	79.5	-7.9	2.5	4.4	6.2
40	86.5	44.9	-4.2	34.2	5.7	11.7
41	51.3	39.5	27.2	40.6	12.2	10.6
42	48.4	38.9	29.9	41.7	12.2	10.9
43	79.9	74.4	1.7	7.6	6.6	5.9
44	54.6	45.0	23.9	36.2	11.2	10.0
45	70.3	55.0	11.8	24.0	8.2	12.7
46	64.3	54.9	16.7	25.1	9.5	11.1
47	69.3	64.9	11.4	18.4	8.6	6.5
48	74.1	65.0	6.9	14.6	7.6	7.4
49	73.0	69.6	7.9	13.6	7.5	6.6
50	58.5	49.6	20.2	31.2	10.0	9.8
51	91.2	79.7	-7.6	2.4	4.6	7.5
52	87.0	79.7	-3.6	2.3	6.0	7.8
53	72.2	64.7	8.6	16.5	7.7	9.2
54	69.4	59.9	10.7	21.4	8.3	9.7
55	49.9	45.0	28.5	36.5	11.9	10.5
56	91.8	79.9	-9.2	2.4	4.3	6.3
57	70.5	38.7	10.0	36.9	8.0	12.7
58	73.7	59.8	4.0	20.4	6.5	8.9
59	87.7	79.6	-4.8	2.3	5.4	7.1
60	67.0	60.0	13.6	22.5	8.7	10.9
61	66.1	49.8	14.7	29.7	9.2	12.8
62	82.9	85.6	-0.4	1.4	6.0	6.3
63	81.6	85.6	-0.4	1.4	6.0	6.3
64	64.4	54.8	16.1	26.8	9.5	9.2
65	78.2	74.4	3.7	10.2	7.0	8.3
66	83.6	85.3	-1.4	1.4	5.8	5.6
67	89.3	85.6	-6.4	1.1	5.0	5.3
68	59.5	44.6	19.9	33.9	10.5	11.1
69	58.9	44.9	21.1	35.4	10.0	10.3
70	45.6	39.8	32.6	40.6	13.3	10.8
71	71.6	64.9	8.6	18.2	8.3	8.4
72	72.7	64.8	7.7	16.1	7.9	9.5
73	80.2	74.2	0.8	8.8	6.4	5.9
74	84.2	85.1	-0.6	1.4	5.8	6.6
75	76.0	69.9	4.8	12.4	6.8	8.3
76	87.5	64.7	-5.0	12.5	5.1	7.9
77	88.2	85.6	-4.8	1.7	4.9	5.4
78	60.8	38.7	18.1	37.0	9.6	11.3
79	54.0	38.7	24.6	37.9	11.4	12.5
80	58.6	44.8	20.7	32.1	10.7	12.2
81	50.6	39.7	28.8	40.5	11.9	9.6
82	53.9	49.9	25.0	28.3	10.8	7.6
83	84.9	84.8	-1.8	1.6	5.4	6.5
84	56.9	50.0	23.2	30.0	10.3	9.1
85	71.1	74.2	10.2	9.4	8.2	8.0
86	75.2	69.7	6.9	14.7	7.4	7.2
87	62.6	54.8	17.5	26.4	9.5	9.2
88	68.7	64.8	11.4	17.9	8.4	7.3
89	73.7	65.0	7.5	16.7	7.8	7.6
90	72.6	59.8	9.1	20.5	7.8	11.3
91	59.7	39.8	20.5	35.4	9.8	13.6
92	59.8	49.6	19.6	28.1	10.2	11.3
93	89.8	79.9	-6.8	2.5	5.2	6.3
94	66.8	59.9	14.2	19.7	8.9	8.6
95	86.5	84.8	-4.1	1.6	5.7	6.4
96	75.0	69.8	6.1	14.5	7.4	7.4
97	67.3	54.9	13.4	24.1	9.1	12.5
98	77.4	74.1	4.6	10.4	6.8	7.0
99	69.1	50.0	12.0	29.2	8.6	11.2
100	68.4	59.9	12.4	20.3	8.8	9.3

Suppementary Table 1

Fig. 4

Suppementary Table 2

Suppementary Table 3

F3 seeds with over 75% oleic acid content detected by NIR for single peanut seed"

样品 Sample	油酸 C18:1 (%)	样品 Sample	油酸 C18:1 (%)	样品 Sample	油酸 C18:1 (%)	样品 Sample	油酸 C18:1 (%)
2-11-2	86.1	8-8-4	83.3	7-3-30	81.6	7-3-4	79.7
9-10-14	85.6	8-8-12	83.2	7-18-22	81.5	8-12-31	79.6
8-12-4	85.6	7-18-2	83.2	7-18-1	81.5	6-2-17	79.5
8-8-14	85.6	3-16-5	83.1	8-8-5	81.5	4-13-2	79.5
2-11-11	85.4	6-1-3	83.1	4-11-1	81.4	3-11-13	79.5
8-12-3	85.3	4-14-23	83.1	11-42-59	81.3	5-12-10	79.4
3-11-4	85.1	8-4-4	83.0	12-21-14	81.3	4-2-8	79.3
9-10-4	85.1	5-12-32	83.0	5-12-40	81.3	4-11-12	79.3
5-12-3	84.9	6-1-22	82.9	3-16-16	81.3	11-31-27	79.3
11-42-2	84.8	9-10-1	82.9	8-4-10	81.3	7-18-32	79.3
10-6-4	84.8	3-3-3	82.9	9-10-20	81.2	4-11-25	79.3
1-11-5	84.7	6-1-23	82.8	11-42-25	81.1	4-13-4	79.1
3-3-2	84.7	4-11-4	82.7	3-16-22	81.1	4-13-7	79.0
5-12-1	84.6	6-2-13	82.7	4-13-14	81.1	8-12-9	78.9
12-21-15	84.6	9-10-5	82.7	4-13-3	81.0	4-13-33	78.9
2-11-5	84.6	11-42-12	82.7	9-10-25	81.0	3-16-45	78.8
10-6-3	84.5	5-12-2	82.6	7-3-1	81.0	3-16-30	78.7
6-1-1	84.4	11-31-22	82.5	4-13-30	81.0	4-13-1	78.7
1-11-4	84.4	4-11-7	82.5	8-12-14	80.9	5-7-21	78.6
4-11-10	84.3	8-12-23	82.4	4-2-1	80.9	7-3-9	78.5
4-2-5	84.2	9-10-33	82.4	10-6-10	80.9	7-3-27	78.5
9-20-4	84.2	7-18-12	82.3	11-31-2	80.8	3-11-2	78.0
1-11-3	84.2	9-21-1	82.3	9-10-27	80.8	11-31-4	78.0
8-8-1	84.2	4-14-5	82.3	9-10-15	80.7	3-11-1	78.0
2-11-3	84.1	11-31-7	82.2	4-2-9	80.7	9-10-26	77.9
2-11-1	84.1	7-18-10	82.2	8-12-7	80.7	3-16-35	77.6
14-14-2	84.1	4-13-24	82.2	11-31-26	80.7	4-14-4	77.6
8-8-17	84.1	5-12-8	82.2	11-31-14	80.7	3-16-24	77.4
4-14-15	84.1	6-2-2	82.2	11-42-19	80.5	11-31-30	77.3
11-31-15	84.1	6-2-9	82.1	3-3-8	80.5	5-12-27	76.5
1-11-1	84.1	9-10-10	82.1	11-31-8	80.5	4-2-42	76.4
2-11-4	84.0	9-10-6	82.1	10-6-18	80.5	4-14-31	76.4
8-8-2	84.0	4-11-5	82.1	2-11-28	80.5	5-7-19	76.3
3-3-5	83.9	6-2-6	82.0	4-11-41	80.3	11-31-28	76.1
9-20-2	83.9	6-1-10	82.0	6-1-7	80.3	8-8-18	75.9
5-7-2	83.9	4-11-3	81.9	4-2-2	80.3	4-13-17	75.8
6-1-4	83.9	6-1-27	81.9	4-13-10	80.3	12-21-10	75.8
10-6-2	83.8	8-4-8	81.9	4-13-34	80.1	6-2-32	75.6
1-11-2	83.7	4-14-14	81.8	9-10-9	80.1	3-3-31	75.6
11-31-3	83.7	8-8-3	81.8	11-42-16	80.1	9-10-16	75.6
9-10-12	83.7	7-3-2	81.7	11-42-8	80.1	3-11-24	75.6
9-10-3	83.6	11-42-1	81.7	12-21-29	80.0	11-31-12	75.5
9-20-3	83.5	7-3-10	81.7	7-18-5	79.9	3-3-19	75.5
12-21-2	83.4	4-14-3	81.7	11-31-17	79.9	5-12-24	75.4
8-12-5	83.4	12-21-1	81.7	4-14-16	79.8	11-42-54	75.2
3-3-1	83.4	11-42-7	81.7	4-14-10	79.8	9-10-37	75.2
6-1-2	83.3	12-21-18	81.6	4-2-35	79.7	9-10-38	75.1
12-21-4	83.3	8-12-34	81.6	4-13-18	79.7	9-10-22	75.0
4-14-1	83.3	6-2-5	81.6	7-3-20	79.7	1-11-9	75.0

Suppementary Table 3

Table 2

Table 3

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	棕榈酸(C16:0)含量 Palmitic acid content	油酸(C18:1) 含量 Oleic acid content	亚油酸(C18:2)含量 Linoleic acid content
样本数 Sample size	1013	1195	1202
最小值Minimum (%)	5.9	32.8	0.7
最大值Maximum (%)	16.6	80.8	43.9
均值Mean (%)	11.1	51.8	26.5

F₄株系 F₄ lines	基因型Genotype
2-11-3	aabb
2002/11/28	aabb
2004/11/7	aabb
4-14-1	aabb
2006/2/6	aabb
2008/8/3	aabb
2009/10/9	aabb
2010/6/4	aabb
11-31-15	aabb
12-21-18	aabb

Establishment and applicant of near-infrared reflectance spectroscopy models for predicting main fatty acid contents of single seed in peanut

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样品 Sample	油酸 C18:1 (%)	样品 Sample	油酸 C18:1 (%)
6-1	82.9	4-13	73.8
2-11	82.2	9-10	73.4
10-6	81.2	5-12	73.3
8-12	80.0	3-16	73.2
3-3	79.7	9-20	73.2
11-31	79.2	5-1	73.2
6-2	78.8	4-12	72.8
7-18	78.3	3-16	72.6
8-8	77.7	10-15	72.5
11-42	76.8	9-11	72.2
4-11	76.2	11-8	72.1
4-14	75.5	4-7	72.1
4-2	75.5	8-19	71.8
5-7	75.1	9-21	71.5
8-4	74.8	4-15	71.3
1-11	74.7	1-16	70.7
7-3	74.4	6-29	70.5
3-11	74.2	2-17	70.3
12-21	74.0	10-7	70.1

	2-11-3^a	2-11-28	4-11-7	4-14-1	6-2-6	8-8-3	9-10-9	10-6-4	11-31-15	12-21-18
1^b	87.5	86.7	77.7	81.2	78.8	78.7	76.5	76.9	75.8	77.0
2	80.3	78.2	76.5	76.7	81.2	76.7	78.7	78.0	79.2	76.6
3	84.2	76.2	76.8	76.4	75.5	76.1	75.4	81.5	79.7	82.9
4	80.6	83.4	80.0	79.0	78.3	80.0	85.8	75.4	76.0	75.8
5	78.1	76.2	77.6	80.8	75.1	78.2	83.7	77.5	86.1	77.3
6	76.3	78.0	79.0	75.9	82.5	76.6	77.8	75.9	75.4	76.5
7	82.0	83.9	77.9	76.7	78.0	81.5	77.5	76.4	76.0	79.7
8	76.7	76.2	77.3	77.5	77.8	77.7	75.2	77.6	81.2	76.6
9	76.1	82.5	82.6	84.1	75.0	79.3	77.5	84.7	83.6	82.9
10	85.1	78.9	80.1	78.3	77.8	76.2	77.5	75.0	79.5	82.2
GC^c	82.0	80.1	80.4	81.7	81.0	81.3	79.7	79.8	80.5	81.2

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