Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (5): 1129-1140.doi: 10.3724/SP.J.1006.2022.12010

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae

ZHU Zheng1,2(), WANG Tian-Xing-Zi1,2, CHEN Yue1,2, LIU Yu-Qing1,2, YAN Gao-Wei1,2, XU Shan1,2, MA Jin-Jiao1,2, DOU Shi-Juan1,2, LI Li-Yun1,2,*(), LIU Guo-Zhen1,2,*()   

  1. 1College of Life Sciences, Hebei Agricultural University, Baoding 071001, Hebei, China
    2Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding 071001, Hebei, China
  • Received:2021-02-10 Accepted:2021-09-09 Online:2022-05-12 Published:2021-10-19
  • Contact: LI Li-Yun,LIU Guo-Zhen E-mail:zhuzheng_mbb@163.com;Liliyun@hebau.edu.cn;gzhliu@hebau.edu.cn
  • Supported by:
    National Natural Science Foundation of China(31171528);National Natural Science Foundation of China(31400700)

RichHTML398

28

PDF

583

Abstract:

Leaf blight is a bacterial disease of rice which leads to heavy yield losses. Xa21 which harbors broad spectrum resistance to leaf blight is the first resistant gene cloned in rice and is widely used in rice breeding program. The identification of transcription factor involved in Xa21-mediated resistance to bacterial leaf blight is of great significance. In this study, transgenic WRKY68-RNAi lines with reduced expression level of WRKY68 protein in the background of Xa21 were generated. The WRKY68-RNAi transgenic lines showed reduced resistance to Xanthomonas oryzae pv. oryzae (Xoo), indicating that WRKY68 played a positive role in Xa21-mediated resistance in rice. No detectable changes of WRKY68 protein abundance in leaves at different stages and positions after inoculation were identified by Western blot, indicating that the expression of WRKY68 protein were independent of Xa21 and Xoo, and thus WRKY68 gene may function as a transcription factor to regulate downstream genes. It was further found that the abundance of a number of pathogenesis-related proteins, including PR1A, PR5, PR10A, PR-pha, and PAL1 were dramatically changed in WRKY68-RNAi transgenic lines when inoculated with Xoo, suggested that these pathogenesis-related proteins were regulated by WRKY68 and the corresponding genes were involved in the downstream defenses responses.

Key words: rice, bacterial leaf blight, resistance gene Xa21, WRKY transcription factor, pathogenesis-related protein

Fig. 1

Identification of WRKY68-RNAi transgenic lines by PCR and WB R#1, R#2, and R#3: independent WRKY68-RNAi transgenic lines; CK: 4021 (transgenic lines of TP309 harbor Xa21 gene); 1-8: transgenic plants in T2 generation; WRKY68: anti-WRKY68 antibody detected band; HSP: anti-HSP82 antibody detected band used as loading control."

Fig. 2

The agronomic traits investigation of WRKY68-RNAi transgenic lines A: plant height; B: the number of tillers; C: 1000-seed weight. CK: 4021 (transgenic lines of TP309 harbor Xa21 gene); R#1, R#2, and R#3: independent WRKY68-RNAi transgenic plants in T2 generation; **: P < 0.01. Error bars indicate the standard derivations."

Fig. 3

Responses of WRKY68-RNAi transgenic lines when inoculated with Xoo A: photographs of leaves at 12 dpi (days post inoculation). B: the development of lesion length on leaves inoculated with Xoo. C: lesion length at 12 dpi. Arrows indicate the frontline of lesions. **: P < 0.01. Bar: 1 cm. R#1, R#2, and R#3: independent WRKY68-RNAi transgenic lines; TP309: wild type; 4021: transgenic lines of TP309 harbor Xa21 gene."

Fig. S1

Abundance Xoo protein in inoculated rice leaves Equal amounts of total protein from multiple samples were resolved by SDS-PAGE. Antibodies: anti-Xoo polyclonal antibody, anti-HSP82 monoclonal antibody. R#2, R#3: WRKY68-RNAi transgenic lines; TP309: wild type; 4021: transgenic TP309 expressing Xa21."

Fig. S2

Expression abundance of pathogenesis-related proteins in leaves inoculated with Xoo Equal amounts of total protein, isolated from inoculated leaves (±1 cm within lesion line) of 5 d or 6 d, were resolved by SDS-PAGE or Tricine-SDS-PAGE. The red frames indicate the portion demonstrated in figure 6. PR1A: anti-PR1a antibody detected band; PR2: anti-PR2 antibody detected band; PR5: anti-PR5 antibody detected band; PR10A: anti-PR10a antibody detected band; PR-pha: anti-PR-pha antibody detected band; PAL1: anti-PAL1 antibody detected band; HSP: anti-HSP82 antibody detected band. R#1, R#2, and R#3: WRKY68-RNAi transgenic lines; TP309: wild type; 4021: transgenic TP309 expressing Xa21."

Fig. 4

Abundance Xoo protein in inoculated rice leaves The whole inoculated leaves were collected, total proteins were isolated and resolved by SDS-PAGE, then detected by anti-Xoo antibody (Fig. S1). Protein signal were detected by Mini Chemiluminescent Imager and Sage Capture software. Lane 1D software was used to extract signals of WB. Average was calculated for three repeats. Error bars are standard deviation (SD). Xoo cells (OD = 0.5) were used as the common reference (CR) to normalized the signal intensity among different WB analysis. R#2, R#3: independent WRKY68-RNAi transgenic lines; TP309: wild type; 4021: transgenic lines of TP309 harbor Xa21 gene."

Fig. 5

Expression profiling of WRKY68 protein in Xoo- inoculated leaves A: the abundance of WRKY68 protein in rice leaves at 3, 5, 7, and 9 dpi, respectively; B: schematic diagram of tissue collection in leaves inoculated with Xoo; C: the abundance of WRKY68 protein at ±1 cm, 1-3 cm, 3-5 cm, and 5-7 cm positions in Xoo-inoculated rice leaves. WRKY68: anti-WRKY68 antibody detected bands; HSP: anti-HSP82 antibody detected bands. TP309: wild type; 4021: transgenic lines of TP309 harbor Xa21 gene."

Fig. 6

Expression abundance of pathogenesis-related proteins in leaves inoculated with Xoo PR1A: anti-PR1A antibody detected band; PR2: anti-PR2 antibody detected band; PR5: anti-PR5 antibody detected band; PR10A: anti-PR10A antibody detected band; PR-pha: anti-PR-pha antibody detected band; PAL1: anti-PAL1 antibody detected band; HSP: anti-HSP82 antibody detected band used as loading control. R#1, R#2, and R#3: independent WRKY68-RNAi transgenic lines; TP309: wild type; 4021: transgenic lines of TP309 harbor Xa21 gene."

[1] Liu W, Liu J L, Triplett L, Leach J E, Wang G L. Novel insights into the rice innate immunity against pathogens. Annu Rev Phytopathol, 2014, 52:213-241.
doi: 10.1146/phyto.2014.52.issue-1
[2] Jones J D G, Dangl J L. The plant immune system. Nature, 2006, 447:323-329.
[3] Shen Y W, Sharma P, Silva F G, Ronald P C. The Xanthomonas oryzae pv. oryzae raxP and raxQ genes encode an ATP sulphurylase and adenosine-5’-phosphosulphate kinase that are required for AvrXa21 avirulence activity. Mol Microbiol, 2002, 44:37-48.
doi: 10.1046/j.1365-2958.2002.02862.x
[4] Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Holsten T, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P C. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270:1804-1806.
doi: 10.1126/science.270.5243.1804 pmid: 8525370
[5] Pruitt R N, Schwessinger B, Joe A, Thomas N, Liu F, Albert M, Robinson M R, Chan L J G, Luu D D, Chen H, Bahar O, Daudi A, Ruan D L, Majumder D, Chern M, Kalbacher H, Midha S, Patil P B, Sonti R V, Petzold C J, Liu C C, Brodbelt J S, Felix G, Ronald P C. The rice immune receptor XA21 recognizes a tyrosine- sulfated protein from a Gram-negative bacterium. Sci Adv, 2015, 1:1500245.
doi: 10.1126/sciadv.1500245 pmid: 26601222
[6] Wang Y S, Pi L Y, Chen X H, Chakrabarty P K, Jiang J, Leon A L D, Liu G Z, Li L C, Benny U, Oard J, Ronald P C, Song W Y, Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance. Plant Cell, 2006, 18:3635-3646.
doi: 10.1105/tpc.106.046730
[7] Peng Y, Bartley L E, Chen X W, Dardick C, Chern M, Ruan R, Canlas P E, Ronald P C. OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice. Mol Plant, 2008, 1:446-458.
doi: 10.1093/mp/ssn024
[8] Park C J, Ronald P C. Cleavage and nuclear localization of the rice XA21 immune receptor. Nat Commun, 2012, 3:920.
doi: 10.1038/ncomms1932
[9] Park C J, Peng Y, Chen X W, Dardick C, Ruan D L, Bart R, Canlas P E, Ronald P C. Rice XB15, a Protein Phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity. PLoS Biol, 2008, 6:231.
[10] Park C J, Wei T, Sharma R, Ronald P C. Overexpression of rice auxilin-like protein, XB21, induces necrotic lesions, up-regulates endocytosis-related genes, and confers enhanced resistance to Xanthomonas oryzae pv. oryzae. Rice, 2017, 10:27.
doi: 10.1186/s12284-017-0166-1
[11] Chen X W, Chern M, Canlas P E, Ruan D L, Jiang C Y, Ronald P C, Baulcombe D C. An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21-mediated immunity. Proc Natl Acad Sci USA, 2010, 107:8029-8034.
doi: 10.1073/pnas.0912311107
[12] Jiang Y N, Chen X H, Ding X D, Wang Y S, Chen Q, Song W Y. The XA21 binding protein XB25 is required for maintaining XA21-mediated disease resistance. Plant J, 2013, 73:814-823.
doi: 10.1111/tpj.2013.73.issue-5
[13] Chen X W, Zuo S M, Schwessinger B, Chern M, Canlas P E, Ruan D L, Zhou X G, Wang J, Daudi A, Petzold C J, Heazlewood J L, Ronald P C. An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors. Mol Plant, 2014, 7:874-892.
doi: 10.1093/mp/ssu003
[14] Park C J, Bart R, Chern M, Canlas P E, Bai W, Ronald P C. Overexpression of the endoplasmic reticulum chaperone BiP3 regulates XA21-mediated innate immunity in rice. PLoS One, 2010, 5:9262.
[15] Agarwal P, Reddy M P, Chikara J. WRKY: its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants. Mol Biol Rep, 2011, 38:3883-3896.
doi: 10.1007/s11033-010-0504-5
[16] Eulgem T, Rushton P J, Robatzek S, Somssich I E. The WRKY superfamily of plant transcription factors. Trends Plant Sci, 2000, 5:199-206.
pmid: 10785665
[17] Qiu D Y, Xiao J, Ding X H, Xiong M, Cai M, Cao Y L, Li X H, Xu C G, Wang S P. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate- dependent signaling. Mol Plant Microbe Interact, 2007, 20:492-499.
doi: 10.1094/MPMI-20-5-0492
[18] Peng Y, Bartley L E, Canlas P, Ronald P C. OsWRKY IIa transcription factors modulate rice innate immunity. Rice, 2010, 3:36-42.
doi: 10.1007/s12284-010-9039-6
[19] Cheng H T, Liu H B, Deng Y, Xiao J H, Li X H, Wang S P. The WRKY45-2 WRKY13 WRKY42 transcriptional regulatory cascade is required for rice resistance to fungal pathogen. Plant Physiol, 2015, 167:1087-1099.
doi: 10.1104/pp.114.256016
[20] Hwang S H, Kwon S I, Jang J Y, Fang I L, Lee H, Choi C, Park S, Ahn I, Bae S C, Hwang D J. OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. Plant Cell Rep, 2016, 35:1975-1985.
doi: 10.1007/s00299-016-2012-0
[21] Liu X Q, Bai X Q, Wang X J, Chu C C. OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol, 2007, 164:969-979.
doi: 10.1016/j.jplph.2006.07.006
[22] Son S, An H K, Seol Y J, Park S R, Im J H. Rice transcription factor WRKY114 directly regulates the expression of OsPR1a and chitinase to enhance resistance against Xanthomonas oryzae pv. oryzae. Biochem Bioph Res Commu, 2020, 533:1262-1268.
[23] Du D, Zhang C W, Xing Y D, Lu X, Cai L J, Yun H, Zhang Q L, Zhang Y Y, Chen X L, Liu M M, Sang X C, Ling Y H, Yang Z L, Li Y F, Lefebvre B, He G H. The CC-NB-LRR OsRLR1 mediates rice disease resistance through interaction with OsWRKY19. Plant Biotechnol J, 2021, 19:1052-1064.
doi: 10.1111/pbi.v19.5
[24] Vo K T X, Kim C Y, Hoang T V, Lee S K, Shirsekar G, Seo Y S, Lee S W, Wang G L, Jeon J S. OsWRKY67 plays a positive role in basal and XA21-mediated resistance in rice. Front Plant Sci, 2018, 8:2220.
doi: 10.3389/fpls.2017.02220
[25] Tao Z, Liu H B, Qiu D Y, Zhou Y, Li X H, Xu C G, Wang S P. A pair of allelic WRKY genes play opposite role in rice-bacteria interactions. Plant Physiol, 2009, 151:936-948.
doi: 10.1104/pp.109.145623
[26] Seo Y S, Chern M, Bartley L E, Han M, Jung K H, Lee I, Walia H, Richter T, Xu X, Cao P J, Bai W, Ramanan R, Amonpant F, Arul L, Canlas P E, Ruan R, Park C J, Chen X W, Hwang S, Jeon J S, Ronald P C. Towards establishment of a rice stress response interactome. PLoS Genet, 2011, 7:e1002020.
[27] Choi C, Hwang S H, Fang I R, Kwon S I, Park S R, Ahn I, Kim J B, Hwang D J. Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens. New Phytol, 2015, 208:846-859.
doi: 10.1111/nph.2015.208.issue-3
[28] Yang S, Zhou L, Miao L Y, Shi J N, Sun C Q, Fan W, Lan J P, Chen H, Liu L J, Dou S J, Liu G Z, Li L Y. The expression and binding properties of the rice WRKY68 protein in the Xa21-mediated resistance response to Xanthomonas oryzae pv. oryzae. J Integr Agric, 2016, 15:2451-2460.
doi: 10.1016/S2095-3119(15)61265-5
[29] Li X M, Bai H, Wang X Y, Li L Y, Cao Y H, Wei J, Liu Y M, Liu L J, Gong X D, Wu L, Liu S Q, Liu G Z. Identification and validation of rice reference proteins for western blotting. J Exp Bot, 2011, 62:4763-4772.
doi: 10.1093/jxb/err084
[30] Guo M C, Lan J P, Shi J N, Guan M L, Wei J, Liu L J, Li L Y, Dou S J, Liu G Z. Western Blot detection of Xanthomonas oryzae pv. oryzae in rice. J Plant Pathol Microb, 2015, (S4):005.
[31] Wu Q, Hou M M, Li L Y, Liu L J, Hou Y, Liu G Z. Induction of pathogenesis-related proteins in rice bacterial resistant gene XA21-mediated interactions with Xanthomonas oryzae pv. oryzae. J Plant Pathol, 2011, 93:455-459.
[32] Hou M M, Xu W J, Bai H, Liu Y M, Li L Y, Liu L J, Liu B, Liu G Z. Characteristic expression of rice pathogenesis-related proteins in rice leaves during interactions with Xanthomonas oryzae pv. oryzae. Plant Cell Rep, 2012, 31:895-904.
doi: 10.1007/s00299-011-1210-z
[33] 陈悦, 王田幸子, 杨烁, 张彤, 马金姣, 燕高伟, 刘玉晴, 周艳, 史佳楠, 兰金苹, 魏健, 窦世娟, 刘丽娟, 杨明, 李莉云, 刘国振. 水稻转录因子OsWRKY68蛋白质的表达特征及其功能特性. 中国农业科学, 2019, 52:2021-2032.
Chen Y, Wang T X Z, Yang S, Zhang T, Ma J J, Yan G W, Liu Y Q, Zhou Y, Shi J N, Lan J P, Wei J, Dou S J, Liu L J, Yang M, Li L Y, Liu G Z. Expression profiling and functional characterization of rice transcription factor OsWRKY68. Sci Agric Sin, 2019, 52:2021-2032.
[34] Chen Q, Huang X E, Chen X H, Shamsunnaher, Song W Y. Reversible activation of XA21-mediated resistance by temperature. Eur J Plant Pathol, 2018, 153:1177-1184.
doi: 10.1007/s10658-018-01634-6
[35] Ross C A, Liu Y, Shen Q X J. The WRKY gene family in rice (Oryza sativa). J Integr Plant Biol, 2007, 49:827-842.
doi: 10.1111/jipb.2007.49.issue-6
[36] Choi N Y, Lee E, Lee S G, Choi C H, Park S R, Ahn I, Bae S C, Hwang C H, Hwang D J. Genome-wide expression profiling of OsWRKY superfamily genes during infection with Xanthomonas oryzae pv. oryzae using real-time PCR. Front Plant Sci, 2017, 8:1628.
doi: 10.3389/fpls.2017.01628
[37] Tariq R, Wang C L, Qin T F, Xu F F, Tang Y C, Gao Y, Ji Z Y, Zhao K J. Comparative transcriptome profiling of rice near- isogenic line carrying Xa23 under Infection of Xanthomonas oryzae pv. oryzae. Int J Mol Sci, 2018, 19:717.
doi: 10.3390/ijms19030717
[38] Nuruzzaman M, Sharoni A M, Satoh K, Kumar A, Leung H, Kikuchi S. Comparative transcriptome profiles of the WRKY gene family under control, hormone-treated, and drought conditions in near-isogenic rice lines reveal differential, tissue specific gene activation. J Plant Physiol, 2014, 171:2-13.
doi: 10.1016/j.jplph.2013.09.010
[39] Satoh K, Saji S, Ito S, Shimizu H, Saji H, Kikuchi S. Gene response in rice plants treated with continuous fog influenced by pH, was similar to that treated with biotic stress. Rice, 2014, 7:10.
doi: 10.1186/s12284-014-0010-9
[40] Ramamoorthy R, Jiang S Y, Kumar N, Venkatesh P N, Srinivasan R. A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. Plant Cell Physiol, 2008, 49:865-879.
doi: 10.1093/pcp/pcn061 pmid: 18413358
[41] Qiu D Y, Xiao J, Xie W B, Cheng H T, Li X H, Wang S P. Exploring transcriptional signaling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice. BMC Plant Biol, 2009, 9:74.
doi: 10.1186/1471-2229-9-74
[42] Zhou C L, Lin Q B, Lan J, Zhang T Y, Liu X, Miao R, Mou C L, Nguyen T, Wang J C, Zhang X, Zhou L, Zhu X J, Wang Q, Zhang X, Guo X P, Liu S J, Jiang L, Wan J M. WRKY transcription factor OsWRKY29 represses seed dormancy in rice by weakening abscisic acid response. Front Plant Sci, 2020, 11:691.
doi: 10.3389/fpls.2020.00691
[43] Zhang C Q, Xu Y, Lu Y, Yu H X, Gu M H, Liu Q Q. The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice. Planta, 2011, 234:541-554.
doi: 10.1007/s00425-011-1423-y
[44] Datta K, Velazhahan R, Oliva N, Ona I, Mew T, Khush G S, Muthukrishnan S, Datta S K. Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. Theor Appl Genet, 1999, 98:1138-1145.
doi: 10.1007/s001220051178
[45] Zhou X G, Liao H C, Chern M, Yin J J, Chen Y F, Wang J P, Zhu X B, Chen Z X, Yuan C, Zhao W, Wang J, Li W T, He M, Ma B, Wang Y W, Wang W M, Wu X J, Li P, Zhu L H, Li S G, Ronald P C, Chen X W. Loss of function of a rice TPR-domain RNA- binding protein confers broad-spectrum disease resistance. Proc Natl Acad Sci USA, 2018, 115:3174-3179.
doi: 10.1073/pnas.1705927115
[46] Solekh R, Susanto F A, Joko T, Nuringtyas T R, Purwestri Y A. Phenylalanine ammonia lyase (PAL) contributes to the resistance of black rice against Xanthomonas oryzae pv. oryzae. J Plant Pathol, 2019, 426:359-365.
[47] Gan Q, Bai H, Zhao X F, Tao Y, Zeng H P, Han Y N, Song W Y, Zhu L H, Liu G Z. Transcriptional characteristics of Xa21- mediated defense responses in rice. J Integr Plant Biol, 2011, 53:300-311.
doi: 10.1111/j.1744-7909.2011.01032.x
[48] Cho M H, Lee S W. Phenolic phytoalexins in rice: biological functions and biosynthesis. Int J Mol Sci, 2015, 16:29120-29133.
doi: 10.3390/ijms161226152
[49] Peng X X, Hu Y J, Tang X K, Zhou P L, Deng X B, Wang H H, Guo Z J. Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice. Planta, 2012, 236:1485-1498.
doi: 10.1007/s00425-012-1698-7
[50] Choi N, Im J H, Lee E, Lee J, Choi C, Park S R, Hwang D J. OsWRKY10 transcriptional regulatory cascades are involved in basal defense and Xa1-mediated resistance. J Exp Bot, 2020, 71:3735-3748.
doi: 10.1093/jxb/eraa135
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[10] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[11] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[12] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[13] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[14] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
[15] WU Yan-Fei, HU Qin, ZHOU Qi, DU Xue-Zhu, SHENG Feng. Genome-wide identification and expression analysis of Elongator complex family genes in response to abiotic stresses in rice [J]. Acta Agronomica Sinica, 2022, 48(3): 644-655.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd