Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2090-2097.doi: 10.3724/SP.J.1006.2014.02090

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Functional Characterization of the Glycoside Hydrolase Encoding Gene OsBE1 during Chloroplast Development in Oryza sativa

WANG Xing-Chun1,2,*,WANG Min1,#,JI Zhi-Juan3,CHEN Zhao4,LIU Wen-Zhen3,HAN Yuan-Huai2,YANG Chang-Deng3,*   

  1. 1 College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China; 2 Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801, China; 3 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 4 College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, China
  • Received:2014-07-16 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-20
  • Contact: 王兴春, E-mail: wxingchun@163.com, Tel: 0354-6287191-307; 杨长登, E-mail: yangchangdeng@yahoo.com.cn, Tel: 0571-63370367

Abstract:

Chloroplast plays an important role in plant carbohydrate metabolism; however the function of carbohydrate metabolism in the chloroplast development is poorly understood. The Oryza sativa Branching Enzyme 1 (OsBE1) gene, encoding a glycoside hydrolase family 13 protein, was cloned from rice. The identity between OsBE1 and Arabidopsis AtBE1 is 66%, but only 40% between OsBE1 and the classical starch branching enzymes in rice. A T-DNA insertion mutant of OsBE1 gene was identified. The seedlings of the osbe1 mutant were albino, and died at the three-leaf stage. This albino phenotype could not be rescued by exogenous carbohydrate. Starch staining showed no obvious difference in starch content between osbe1 and the wild type. Further studies showed that there were less chloroplasts in osbe1 mutant than in wild type, and there was no obvious stroma lamella in the osbe1 chloroplast. The overexpression vector pCAMBIA1300-35S-OsBE1 was constructed and transformed into rice variety Zhonghua 11. Finally, 108 transgenic lines were obtained and 77 lines of them showed etiolation in different degrees. The work not only sheds a novel insight into the regulation mechanism of carbohydrate metabolism on chloroplast development, but also lays a foundation for further understanding of the OsBE1’s function.

Key words: Glycoside hydrolase, Carbohydrate metabolism, OsBE1, Chloroplast, Rice (Oryza sativa L.)

[1]何美敬, 刘立峰, 穆国俊, 侯名语, 陈焕英, 崔顺立. 花生蔗糖合酶基因AhSuSy的克隆和干旱胁迫表达分析. 作物学报, 2012, 38: 2139–2146



He M J, Liu L F, Mu G J, Hou M Y, Chen H Y, Cui S L. Molecular cloning of sucrose synthase gene and expression analysis under drought stress in Peanut (Arachis hypogaea L.). Acta Agron Sin, 2012, 38: 2139–2146 (in Chinese with English abstract)



[2]Wang X C, Xue L, Sun J Q, Zuo J R. The Arabidopsis BE1 gene, encoding a putative glycoside hydrolase localized in plastids, plays crucial roles during embryogenesis and carbohydrate metabolism. J Integr Plant Biol, 2010, 52: 273–288



[3]Wang X C, Yang Z R, Wang M, Meng L Z, Jiang Y W, Han Y H. The BRANCHING ENZYME1 gene, encoding a glycoside hydrolase family 13 protein, is required for in vitro plant regeneration in Arabidopsis. Plant Cell Tissue Organ Cult, 2014,117: 279–291



[4]Nashilevitz S, Melamed-Bessudo C, Aharoni A, Kossmann J, Wolf S, Levy A A. The legwd mutant uncovers the role of starch phosphorylation in pollen development and germination in tomato. Plant J, 2009, 57: 1–13



[5]Lee S K, Jeon J S, Bornke F, Voll L, Cho J I, Goh C H, Jeong S W, Park Y I, Kim S J, Choi S B, Miyao A, Hirochika H, An G, Cho M H, Bhoo S H, Sonnewald U, Hahn T R. Loss of cytosolic fructose-1,6-bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa). Plant Cell Environ, 2008, 31: 1851–1863



[6]Niittyla T, Messerli G, Trevisan M, Chen J, Smith A M, Zeeman S C. A previously unknown maltose transporter essential for starch degradation in leaves. Science, 2004, 303: 87–89



[7]Sulpice R, Pyl E T, Ishihara H, Trenkamp S, Steinfath M, Witucka-Wall H, Gibon Y, Usadel B, Poree F, Piques M C, Von Korff M, Steinhauser M C, Keurentjes J J, Guenther M, Hoehne M, Selbig J, Fernie A R, Altmann T, Stitt M. Starch as a major integrator in the regulation of plant growth. Proc Natl Acad Sci USA, 2009, 106: 10348–10353



[8]Dumez S, Wattebled F, Dauvillee D, Delvalle D, Planchot V, Ball S G, D'Hulst C. Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation. Plant Cell, 2006, 18: 2694–2709



[9]Labes A, Karlsson E N, Fridjonsson O H, Turner P, Hreggvidson G O, Kristjansson J K, Holst O, Schonheit P. Novel members of glycoside hydrolase family 13 derived from environmental DNA. Appl Environ Microbiol, 2008, 74: 1914–1921



[10]Jeong D H, An S, Park S, Kang H G, Park G G, Kim S R, Sim J, Kim Y O, Kim M K, Kim S R, Kim J, Shin M, Jung M, An G. Generation of a flanking sequence-tag database for activation-tagging lines in japonica rice. Plant J, 2006, 45: 123–132



[11]An S, Park S, Jeong D H, Lee D Y, Kang H G, Yu J H, Hur J, Kim S R, Kim Y H, Lee M, Han S, Kim S J, Yang J, Kim E, Wi S J, Chung H S, Hong J P, Choe V, Lee H K, Choi J H, Nam J, Kim S R, Park P B, Park K Y, Kim W T, Choe S, Lee C B, An G. Generation and analysis of end sequence database for T-DNA tagging lines in rice. Plant Physiol, 2003, 133: 2040–2047



[12]Jeon J S, Lee S, Jung K H, Jun S H, Jeong D H, Lee J, Kim C, Jang S, Yang K, Nam J, An K, Han M J, Sung R J, Choi H S, Yu J H, Choi J H, Cho S Y, Cha S S, Kim S I, An G. T-DNA insertional mutagenesis for functional genomics in rice. Plant J, 2000, 22: 561–570



[13]Wang W, Wu C, Liu M, Liu X R, Hu G C, Si H M, Sun Z X, Liu W Z, Fu Y P. Resistance of antimicrobial peptide genes transgenic rice to bacterial blight. Rice Sci, 2011, 1: 10–16



[14]Finn R D, Bateman A, Clements J, Coggill P, Eberhardt R Y, Eddy S R, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer E L, Tate J, Punta M. Pfam: the protein families database. Nucl Acids Res, 2014, 42: D222–230



[15]Emanuelsson O, Nielsen H, von Heijne G. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci, 1999, 8: 978–984



[16]Han Y, Sun F J, Rosales-Mendoza S, Korban S S. Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants. Gene, 2007, 401: 123–130



[17]James M G, Denyer K, Myers A M. Starch synthesis in the cereal endosperm. Curr Opin Plant Biol, 2003, 6: 215–222



[18]Yun M S, Umemoto T, Kawagoe Y. Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis. Plant Cell Physiol, 2011, 52: 1068–1082

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!