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作物学报 ›› 2013, Vol. 39 ›› Issue (09): 1582-1593.doi: 10.3724/SP.J.1006.2013.01582

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系

潘晓飚1,**,陈凯2,3,**,张强2,3,黄善军1,谢留杰1,李美4,孟丽君4,徐正进3,徐建龙2,4,*,黎志康2,4   

  1. 1浙江省台州市农业科学研究院, 浙江临海 317000; 2中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程, 北京 100081;
    3沈阳农业大学 / 农业部作物生理生态遗传育种重点开放实验室, 辽宁沈阳 110161; 4中国农业科学院深圳生物育种创新研究院, 广东深圳 518083
  • 收稿日期:2013-01-14 修回日期:2013-06-04 出版日期:2013-09-12 网络出版日期:2013-07-09
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)(2011-G2B), 国家高技术研究发展计划(863计划)(2012AA101101)和浙江省公益技术研究项目(2012C22033)资助。

Developing Restorer Lines Pyramiding Different Resistant Genes to Blast and Bacterial Leaf Blight by Marker-assisted Selection in Rice

PAN Xiao-Biao1,**,CHEN Kai2,3,**,ZHANG Qiang2,3,HUANG Shan-Jun1,XIE Liu-Jie1,LI Mei4,Meng Li-Jun2,4,XU Zheng-Jin3,XU Jian-Long2,4,*,LI Zhi-Kang2,4   

  1. 1 Taizhou Academy of Agricultural Sciences of Zhejiang Province, Linhai 317000, China; 2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China; 4 Shenzhen Institute of Biological Breeding & Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518083, China?
  • Received:2013-01-14 Revised:2013-06-04 Published:2013-09-12 Published online:2013-07-09

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摘要：

利用分子标记辅助选择和田间鉴定选择相结合的方法, 将三黄占2号的抗稻瘟病主基因Pi-GD-1(t)、Pi-GD-2(t)和主效QTL GLP8-6(t) (分别简称G1、G2和G8)及抗白叶枯病基因Xa23导入到明恢86、蜀恢527和浙恢7954等3个骨干中籼恢复系, 通过复交进行基因聚合, 获得5个带有抗稻瘟病兼抗白叶枯病的双基因或多基因聚合系明恢86-G1-G2-Xa23、蜀恢527-G2-Xa23、明浙-G2-G8-Xa23-1、明浙-G2-G8-Xa23-2和明浙-G1-G2-G8-Xa23。以上5个抗病基因聚合改良系对稻瘟病的抗谱与抗源品种相仿或更宽, 改良系和与不育系II-32A配制的测交种对白叶枯病菌的抗谱与供体亲本IRBB23一致, 测交种在不接种白叶枯病菌条件下的产量和结实率与原来的恢复系及相应杂交种相仿, 但在接种条件下带有Xa23基因的恢复系及测交种的结实率、千粒重和产量明显优于原来的恢复系及相应杂交种。研究表明, 抗稻瘟病基因和抗白叶枯基因Xa23在不同恢复系背景下的抗性表达完全, 对恢复系稻瘟病以及白叶枯病改良的效果明显。对分子标记复交改良恢复系的抗病性进行了讨论。

关键词: 水稻恢复系, 稻瘟病, 白叶枯病, 抗性改良, 标记辅助选择

Abstract:

The novel blast resistance genes, Pi-GD-1(t), Pi-GD-2(t), and GLP8-6(t) from rice variety Sanhuangzhan2 (here abbreviated them as G1, G2 and G8, respectively) and bacterial blight (BB) resistance gene, Xa23 were introgressed into three elite restorer lines (Minghui86, Shuhui527 and Zhehui7954) by marker-assisted pyramiding breeding approaches in combination with artificial inoculation and stringent phenotypic selections. The five derived blast and bacterial blight resistance restorer lines, Minghui86-G1-G2-Xa23, Shuhui527-G2-Xa23, Mingzhe-G2-G8-Xa23-1, Mingzhe-G2-G8-Xa23-2 and Mingzhe-G1-G2-G8-Xa23, demonstrated similar or wider blast resistance spectrum as compared with the donor parent, Sanhuangzhan 2. The five derived BB resistant restorer lines and their derived hybrid combinations with II-32A demonstrated similar BB resistance spectrum to the donor parent, IRBB23. The newly developed BB resistant restorers and their derived hybrids were identical to their respective original versions for agronomic traits especially under disease free condition. However, under severe disease condition, the five BB resistant restorer lines exhibited significantly higher grain weight, spikelet fertility and grain yield as compared with the respective original restorer lines, thus further resulting in significantly higher grain yields in BB resistant hybrids than in their respective original hybrids. Improvement of resistance of blast and BB for restorer lines by molecular marker-assisted pyramiding was discussed.

Key words: Rice restorer line, Blast, Bacterial leaf blight, Resistance improvement, Marker assisted selection (MAS)

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