欢迎访问作物学报,今天是

作物学报 ›› 2009, Vol. 35 ›› Issue (3): 560-565.doi: 10.3724/SP.J.1006.2009.00560

• 研究简报 • 上一篇    下一篇

持绿型高粱B35灌浆期对干旱的生理生化响应

黄瑞冬;孙璐;肖木辑;周宇飞   

  1. 沈阳农业大学农学院,辽宁沈阳110161
  • 收稿日期:2008-06-24 修回日期:2008-10-18 出版日期:2009-03-12 网络出版日期:2009-01-16
  • 通讯作者: 黄瑞冬,r_huang@126.com; Tel: 024-88487135
  • 基金资助:

    本研究由国家科技支撑计划项目(2006BAD02B00),辽宁省教育厅资助项目(20060768)资助

Physiological and Biochemical Responses to Drought during filling Stage in Stay Green Sorghum B35

HUANG Rui-Dong;SUN Lu;XIAO Mu-Ji;ZHOU Yu-Fei   

  1. College of Agronomy,Shenyang Agricultural University,Shenyang 110161,China
  • Received:2008-06-24 Revised:2008-10-18 Published:2009-03-12 Published online:2009-01-16

摘要:

在盆栽条件下, 对典型持绿型高粱品系B35在开花后10 d和开花后30 d进行1周的干旱胁迫, 胁迫结束后测定与持绿性或抗旱性相关的生理生化指标。结果表明, 持绿型高粱品系B35与对照普通高粱品系三尺三相比, 具有较高的叶绿素含量(SPAD值)、净光合速率(Pn)和叶片、茎秆、籽粒含氮量, 且干旱胁迫使其值下降幅度较小。与对照相比, 干旱胁迫条件使B35叶片的相对电导率和丙二醛(MDA)含量增加量较少, 游离脯氨酸含量的增加量较多, 叶水势下降幅度较大。B35的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均较高。因此, 我们认为在干旱逆境中持绿型高粱品系B35能够在光合作用、氮素积累、叶片质膜透性、渗透调节能力和保护酶活性等方面有效进行自身的生理调节, 以适应干旱胁迫。

关键词: 高粱, 持绿性, 抗旱性, 生理生化特性

Abstract:

Effects of drought on a typical stay green sorghum line B35 were investigated through analyzing the physiological and biochemical characteristics of sorghum plants with the treatments of weekly drought stress starting on the 10th day and the 30th day after anthesis respectively in pot experiment. Compared with CK, the stay green sorghum B35 showed higher chlorophyll content (SPAD value), photosynthetic rate and nitrogen content in leaf, stem and grain. Under the condition of drought stress, the increments of electrical conductivity rate, malondialdehyde content in B35 were less than those in CK, the increments of proline contents and decrements of leaf water potential in B35 were more than those in CK. The activities of superoxide dismutase (SOD) and peroxydase (POD) of B35 were higher than those of CK. We suggested that the stay green sorghum line could perform the physiological autoregulation effectively at the aspects of photosynthesis, nitrogen accumulation, cell membrane permeability of leaf, osmoregulation ability and activities of protective enzymes to adapt the drought.

Key words: Sorghum, Stay green, Drought resistance, Physiological and biochemical characteristics

[1]Rosenow D T, Quisenberry J E, Wendt C W. Drought tolerant sorghum and cotton germplasm. Agric Water Manag, 1983, 7: 207–222
[2]Borrell A K, Hammer G L. Nitrogen dynamics and the physiological basis of stay-green in sorghum. Crop Sci, 2000, 40: 1295–1307
[3]Duncan R R, Bockhoit A J, Miler F R. Descriptive comparison of senescent and nonsenescent sorghum genotypes. Agron J, 1981, 73: 849–853
[4]Walulu R S, Rosenow D T, Wester D B, Nguyen H T. Inheritance of the stay green trait in sorghum. Crop Sci, 1994, 34: 970–972
[5]Gentinetta E, Ceppi D, Perico G, Motto M, Salamini F. A major gene for delayed senescence in maize: Pattern of photosynthates accumulation and inheritance. Plant Breed, 1986, 97: 193–203
[6]Spano G, Di Fonzo N, Perrotta C, Platani C, Ronga G, Lawlor DW, Napier J A, Shewry P R. Physiological characterization of 'stay-green' mutants in durum wheat. J Exp Bot, 2003, 54: 1415–1420
[7]Pierce R O, Knowles P F, Phillips D A. Inheritance of delayed leaf senescence in soybean. Crop Sci, 1984, 24: 515–518
[8]Yue B, Xue W, Luo L. QTL Analysis for flag leaf characteristics and their relationships with yield and yield traits in rice. Acta Genet Sin, 2006, 33: 824–832
[9]Sun L(孙璐), Huang R-D(黄瑞冬). Research on the root morphological and physiological characteristics of stay green sorghum. J Anhui Agric Sci (安徽农业科学), 2007, 35(15): 4433–4434(in Chinese with English abstract)
[10]Xu W-H(徐文华), Wang K-J(王空军), Wang Y-J(王永军), Zhao J-R(赵久然), Dong S-T(董树亭), Hu C-H(胡昌浩). Comparison of yield and nutritive value in sorghum, sudangrass and sorghum sudangrass hybrd. Acta Agron Sin (作物学报), 2006, 32(8): 1218–1222(in Chinese with English abstract)
[11]Ge J-L(葛江丽), Shi L(石雷), Gu W-B(谷卫彬), Tang Y-D(唐宇丹), Zhang J-Z(张金政), Jiang C-D(姜闯道), Ren D-M(任大明). Photosynthetic characteristics and the regulation of photosystem II function in salt-stressed sweet sorghum seedlings. Acta Agron Sin (作物学报), 2007, 33(8): 1272–1278(in Chinese with English abstract)
[12]Zhang X-Z(张宪政). Crop Physiological Approach(作物生理研究法). Beijing: Agriculture Press, 1992(in Chinese)
[13]Feng X-M(冯学民), Zhou H-F(周洪飞). Experiment and Statistics (试验与统计). Harbin: Harbin Engineering University Press, 2002(in Chinese)
[14]Xu W, Rosenow D T, Nguyen H T. Stay green trait in grain sorghum: Relationship between visual rating and leaf chlorophyll concentration. Plant Breed, 2000, 119: 365–367
[15]Thomas H, Rogers L J. Turning over an old leaf. Univ Wales Rev Sci Technol, 1989, 6: 29–38
[16]Borrell A K, Hammer G L, Van Oosterom E. Stay-green: A consequence of the balance between supply and demand for nitrogen during grain filling? Ann Appl Biol, 2001, 138: 91–95
[17]Thomas H, Smart C M. Crops that stay green. Appl Biol, 1993, 123: 193–219
[18]Bai X-L(白相历), Qi H(齐华), Liu M(刘明), Zhang Z-P(张振平). Study on the relationship between drought resistance and physiological index of maize. J Maize Sci (玉米科学), 2007, 15(5): 79–83(in Chinese with English abstract)
[19]Li N-N(李娜娜), Hao J-P(郝建平), Yang J-Z(杨锦忠), Yan X-B(阎心柏). Effects of water-permeability plastic film mulching on physiological characteristics of sorghum in drought land. J Shanxi Agric Univ (Nat Sci Edn)(山西农业大学学报·自然科学版), 2007, 27(2): 146–148 (in Chinese with English abstract)
[20]Ma R-K(马瑞昆), Jia X-L(贾秀领), Zhang Q-G(张全国), Zhang L-H(张丽华), Yao Y-R(姚艳荣), Yang L-H(杨利华). Physiological characteristics of water in wheat cultivar SX733: The effect of water-saving irrigation. Acta Agron Sin (作物学报), 2007, 33(9): 1446–1451 (in Chinese with English abstract)
[21]Yan Y(阎勇), Luo X-L(罗兴录), Zhang X-S(张兴思), Lan W-B(兰旺彬). Comparison of maize physiological characters under different treatment of irrigation. Chin Agric Sci Bull (中国农学通报), 2007, 23(9): 323–326(in Chinese with English abstract)
[1] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[2] 付江鹏, 柳发财, 闫宝琴, 王永栋, 李利利, 魏玮, 周英霞. 控释肥替代普通尿素对旱作高粱干物质积累分配、产量和品质的影响[J]. 作物学报, 2025, 51(9): 2501-2513.
[3] 尹雨萌, 王雁楠, 康志河, 乔守晨, 卞倩倩, 李亚蔚, 曹郭郑, 赵国瑞, 徐丹丹, 杨育峰. 甘薯谷胱甘肽S-转移酶基因IbGSTU7的克隆及功能分析[J]. 作物学报, 2025, 51(7): 1736-1746.
[4] 金欣欣, 宋亚辉, 苏俏, 杨永庆, 李玉荣, 王瑾. 冀花系列高油酸花生抗旱性鉴定与综合评价[J]. 作物学报, 2025, 51(3): 797-811.
[5] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 张立异. 中国高粱株高和节间数全基因组关联分析及候选基因预测[J]. 作物学报, 2025, 51(3): 568-585.
[6] 王语新, 陈天羽, 翟红, 张欢, 高少培, 何绍贞, 赵宁, 刘庆昌. 甘薯激酶基因IbHT1的克隆及抗旱性功能鉴定[J]. 作物学报, 2025, 51(2): 301-311.
[7] 何梅, 张佳蕾, 范士凯, 孟静静, 王建国, 郭峰, 李新国, 万书波, 杨莎. 花生抗旱基因AhCPK8及其互作蛋白GAPDH的克隆及分子鉴定[J]. 作物学报, 2025, 51(10): 2713-2726.
[8] 陈昊翔, 万鑫杰, 陈青, 王劲松, 董二伟, 王媛, 黄晓磊, 刘秋霞, 焦晓燕. 石灰性褐土条件下高粱与玉米耐瘠薄特性及对土壤肥力响应的比较[J]. 作物学报, 2025, 51(10): 2775-2787.
[9] 刘波, 池明, 曹梦琦, 唐达, 杨恒照, 张卫华, 薛聪. 过表达马铃薯StuPPO9基因对烟草抗旱能力的影响[J]. 作物学报, 2024, 50(9): 2237-2247.
[10] 孙美红, 王彩琴, 王蓉, 白文斌, 高振峰. Bacillus velezensis G-1·脂肽悬浮种衣剂的制备及其对高粱丝黑穗病菌的防侵染效果[J]. 作物学报, 2024, 50(6): 1467-1485.
[11] 陈玉章, 吴松果, 卢成霖, 李瑞, 龚利娟, 文悦, 宁佳欣, 吴宇涵. 条带垄覆对西南黄壤坡耕地径流和高粱水分利用的影响[J]. 作物学报, 2024, 50(5): 1325-1340.
[12] 王瑞, 张福耀, 詹鹏杰, 楚建强, 晋敏姗, 赵威军, 程庆军. 基于RNA-Seq筛选高粱低氮胁迫相关候选基因[J]. 作物学报, 2024, 50(3): 669-685.
[13] 房孟颖, 任粱, 卢霖, 董学瑞, 武志海, 闫鹏, 董志强. 乙矮合剂对粒用高粱根系建构和产量的影响[J]. 作物学报, 2023, 49(9): 2528-2538.
[14] 王媛, 王劲松, 董二伟, 刘秋霞, 武爱莲, 焦晓燕. 施氮量对高粱籽粒灌浆及淀粉累积的影响[J]. 作物学报, 2023, 49(7): 1968-1978.
[15] 陆梦琪, 谢若涵, 李想, 杨明冲, 何紫薇, 高杰, 赵晓燕, 沈祥陵, 陈燕, 王继斌, 胡利华, 段明政, 王令强. 基于“LabelmeP1.0”提取的高粱维管束参数与农艺性状关联研究[J]. 作物学报, 2023, 49(7): 1954-1967.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!