欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (07): 1092-1099.doi: 10.3724/SP.J.1006.2010.01092

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

开花后不同光周期条件下大豆农艺性状和品质性状的QTL分析

王英,程立锐**,冷建田,吴存祥,侯文胜,韩天富*   

  1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081
  • 收稿日期:2009-12-28 修回日期:2010-03-19 出版日期:2010-07-12 网络出版日期:2010-04-28
  • 通讯作者: 韩天富, E-mail: hantf@mail.caas.net.cn; Tel: 010-82108784
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2009CB118400),国家高技术研究发展计划(2006AA100104)和农业部、财政部现代农业产业技术体系建设专项资金资助(nycytx-004).

QTL Mapping of Agronomic and Quality Traits in Soybean under Different Post-Flowering Photoperiods

WANG Ying,CHENG Li-Rui**,LENG Jian-Tian,WU Cun-Xiang,HOU Wen-Sheng,HAN Tian-Fu*   

  1. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2009-12-28 Revised:2010-03-19 Published:2010-07-12 Published online:2010-04-28
  • Contact: HAN Tian-Fu, E-mail: hantf@mail.caas.net.cn; Tel: 010-82108784

PDF

2064

被引次数

18

摘要： 以开花期相近的181个大豆重组自交系(RIL)为材料，研究开花后不同光照长度对大豆主要农艺性状的影响，并在利用SSR标记构建大豆遗传图谱的基础上，分别在长日(16 h)和短日(12 h)条件下检测与主要农艺性状及其光周期敏感度(PS)相关的QTL。结果表明，开花后光照处理对大豆农艺性状和品质性状有较大影响，不同性状的光周期敏感度差异明显，株高＞主茎节数＞蛋白质含量、脂肪含量＞百粒重＞单株荚数＞蛋白质和脂肪总量。利用复合区间作图法检测到12个与株高、主茎节数、单株荚数、百粒重、蛋白质和脂肪总量等性状及各性状对开花后光周期处理的敏感度相关的QTL，分别定位于A1、A2、B1、B2、C1、D1a、F、L等8个连锁群上。其中，在短日条件下检测到4个QTL，可解释的遗传变异范围在11.37%~26.63%之间；在长日条件下检测到3个QTL，可解释的遗传变异范围在11.84%~27.85%之间；检测到5个与不同性状光周期敏感度有关的QTL，可解释相对应性状表型变异的范围在6.15%~21.44%之间。针对同一性状，未检测到在长日和短日条件下均起作用的主效QTL, 说明开花后光周期对大豆产量和品质性状相关基因的表达有较大影响。

关键词: 大豆, 开花后光周期, 农艺性状, 品质性状, QTL定位

Abstract: Most of agronomic and quality traits of soybean (Glycine max L. Merr.) are quantitatively inherited, and sensitive to photoperiod. In this study, 181recombinant inbred lines (RIL) of F10 generation were used to analyze the post-flowering photoperiod effects on yield and quality traits of soybean, and 129 SSR markers were used to map QTLs associated with the above traits under both long day (LD) and short day (SD) conditions and their photoperiod sensitivity (PS). The results showed that photoperiod greatly affected the performances of agronomic and quality traits of soybean, and the photoperiod sensitivity of the traits were plant height.node number on the main stem>protein content and oil content>100-seed weight>pod number per plant>total percentage of protein and oil. By using CIM (composite interval mapping) method, a total of 12 QTLs associated with the agronomic and quality traits and their sensitivity to the post-flowering photoperiod were identified and mapped on eight linkage groups including A1, A2, B1, B2, C1, D1a, F and L. Among the QTLs mentioned above, four were identified under SD and could explain 11.37%–26.63% of phenotypic variation, and three were identified under LD condition and could explain 11.84%–27.85% of phenotypic variation. The other five were associated with photoperiod sensitivity of various traits and could explain 6.15%–21.44% of phenotypic variation. For the same individual agronomic or quality trait, no major QTL was detected under both SD and LD, indicating that post-flowering photoperiod dramatically regulates the expression of genes determining yield and quality traits, and the agronomic and quality traits of soybean are sensitive to environmental factors. It was drawn that photoperiod sensitivity is a key index for adaptability improvement of soybean. For breeding elite varieties with ideal yield potential, quality and stress tolerance, the photoperiod-insensitive genes related to various traits should be explored and pyramided with other needed genes.

Key words: Soybean, Post-flowering photoperiod, Agronomic trait, Quality trait, QTL mapping

[1] Han T-F(韩天富), Gai J-Y(盖钧镒), Qiu J-X (邱家驯). A comparative study on pre- and post-flowering photoperiod response in various ecotypes of soybeans. Soybean Sci (大豆科学), 1998, 17(2): 129-134 (in Chinese with English abstract)
[2] Wang Y(王英), Wu C-X(吴存祥), Zhang X-M(张学明), Wang Y-P(王云鹏), Han T-F(韩天富). Effects of soybean major maturity genes under different photoperiods. Acta Agron Sin (作物学报), 2008, 34(7): 1160-1168 (in Chinese with English abstract)
[3] Han T-F(韩天富), Wang J-L(王金陵). Studies on the post-flowering photoperiodic responses in soybean. Acta Bot Sin (植物学报), 1995, 37(11): 863-869 (in Chinese with English abstract)
[4] Wu C, Ma Q, Yam K M, Cheung M Y, Xu Y, Han T, Lam H M, Chong K. In situ expression of the GmNMH7 gene is photoperiod-dependent in a unique soybean (Glycine max L. Merr.) flowering reversion system. Planta, 2006, 223: 725-735
[5] Kantolic A G, Slafer G A. Reproductive development and yield components in indeterminate soybean as affected by post-flowering photoperiod. Field Crops Res, 2005, 93: 212-222
[6] Han T-F(韩天富), Wang J-L(王金陵). A study on the responses of different ecotypes of Chinese soybeans to post-flowering photoperiod. Acta Agron Sin (作物学报), 1996, 22(1): 20-26(in Chinese with English abstract)
[7] Han T-F(韩天富), Wang J-L(王金陵), Fan B-B(范彬彬), Yao W-Q(姚文秋), Yang Q-K(杨庆凯). Effect of post-flowering daylength on agronomic characters of soybean. Chin J Appl Ecol (应用生态学报), 1996, 7(2): 169-173 (in Chinese with English abstract)
[8] Kantolic A G, Slafer G A. Development and seed number in indeterminate soybean as affected by timing and duration of exposure to long photoperiods after flowering. Ann Bot, 2007, 99: 925-933
[9] Kumudini S V, Pallikonda P K, Steele C. Photoperiod and E-genes influence the duration of the reproductive phase in soybean. Crop Sci, 2007, 47: 1510-1517
[10] Boote K J. Response of soybeans in different maturity groups to March planting in southern USA. Agron J, 1981, 73: 854-859
[11] Smith J R, Nelson R L. Relationship between seed-filling period and yield among soybean breeding lines. Crop Sci, 1986, 26: 469-472
[12] Sarwar G. Impact of grain filling period and other morphological traits in soybean. Soybean Genet Newsl, 1996, 23: 112-116
[13]Curtis D F, Tanner J W, Luzzi B M, Hume D J. Agronomic and phenological differences of soybean isolines differing in maturity and growth habit. Crop Sci, 2000, 40: 1624-1629
[14] Han T-F(韩天富), Wang J-L(王金陵), Yang Q-K(杨庆凯), Gai J-Y(盖钧镒). Effects of post-flowering photoperiod on chemical composition of soybeans. Sci Agric Sin (中国农业科学), 1997, 30(2): 47-53 (in Chinese with English abstract)
[15] Orf J H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642-1651
[16] Hyten D L, Pantalone V R, Sams C E, Saxton A M, Landau-Ellis D, Stefaniak T R. Seed quality QTL in a prominent soybean population. Theor Appl Genet, 2004, 109: 552-561
[17] Xu P(徐鹏), Wang H(王慧), Li Q(李群), Gai J-Y(盖钧镒), Yu D-Y(喻德跃). Mapping QTLs related to oil content of soybeans. Hereditas (遗传), 2007, 29(1): 92-96 (in Chinese with English abstract)
[18] Sun D, Li W, Zhang Z, Chen Q, Ning H, Qiu L, Sun G. Quantitative trait loci analysis for the developmental behavior of Soybean (Glycine max L. Merr.). Theor Appl Genet, 2006, 112: 665-673
[19] Csanádi G, Vollmann J, Stift G, Lelley T. Seed quality QTLs identified in a molecular map of early maturing soybean. Theor Appl Genet, 2001, 103: 912-919
[20] Wu X -L(吴晓雷), Wang Y-J(王永军), He C-Y(贺超英), Chen S-Y(陈受宜), Gai J-Y(盖钧镒), Wang X-C(王学臣). QTLs mapping of some agronomic traits of soybean. Acta Genet Sin (遗传学报), 2001, 28(10): 947-955 (in Chinese with English abstract)
[21] Chung J, Babka H L, Graef G L, Staswick P E, Lee D J, Cregan P B, Shoemaker R C, Specht J E. The seed protein, oil, and yield QTL on soybean linkage group I. Crop Sci, 2003, 43: 1053-1067
[22] Zhang W K, Wang Y J, Luo G Z, Zhang J S, He C Y, Wu X L, Gai J Y, Chen S Y. QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers. Theor Appl Genet, 2004, 108: 1131-1139
[23] Zheng Y-Z(郑永战), Gai J-Y(盖钧镒), Lu W-G(卢为国), Li W-D(李卫东), Zhou R-B(周瑞宝), Tian S-J(田少君). QTL mapping for fat and fatty acid composition contents in soybean. Acta Agron Sin (作物学报), 2006, 32(12): 1823-1830 (in Chinese with English abstract)
[24] Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕), Xin D-W(辛大伟), Shan D-P(单大鹏), Qiu H-M (邱红梅), Shan C-Y(单彩云). QTL analysis of major agronomic traits in soybean. Sci Agric Sin (中国农业科学), 2007, 40(1): 41-47(in Chinese with English abstract)
[25] Wang X-Z(王贤智), Zhang X-J(张晓娟), Zhou R(周蓉), Sha A-H(沙爱华), Wu X-J(吴学军), Cai S-P(蔡淑平), Qiu D-Z(邱德珍), Zhou X-A(周新安). QTL analysis of seed and pod traits in soybean RIL population. Acta Agron Sin (作物学报), 2007, 33(3): 441-448(in Chinese with English abstract)
[26] Chapman A, Pantalone V R, Ustun A, Allen F L, Landau-Ellis D, Trigiano R N, Gresshoff P M. Quantitative trait loci for agronomic and seed quality traits in an F2 and F4:6 soybean population. Euphytica, 2003, 129: 387-393
[27] Mian M A R, Ashley D A, Vencill W K, Boerma H R. QTLs conditioning early growth in a soybean population segregating for growth habit. Theor Appl Genet, 1998, 97: 1210-1216
[28] Panthee D R, Pantalone V R, West D R, Saxton A M, Sams C E. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean. Crop Sci, 2005,45: 2015-2022
[29] Lee S H, Bailey M A, Mian M A R, Shipe E R, Ashley D A, Parrott W A, Hussey R S, Boerma H R. Molecular markers associated with soybean plant height, lodging, and maturity across locations. Crop Sci, 1996, 36: 728-735
[30] Xin D W, Qiu H M, Shan D P, Shan C Y, Liu C Y, Hu G H, Staehelin C, Chen Q S. Analysis of quantitative trait loci underlying the period of reproductive growth stages in soybean (Glycine max L. Merr.). Euphytica, 2008, 162: 155-165
[31] Tasma I M, Lorenzen L L, Green D E, Shoemaker R C. Mapping genetic loci for flowering time, maturity, and photoperiod insensitivity in soybean. Mol Breed, 2001, 8: 25-35
[32] Han T-F(韩天富), Gai J-Y(盖钧镒), Chen F-Y(陈风云), Qiu J-X(邱家驯). Photoperiod response and agronomic characters of soybean varieties with different growth period structures. Acta Agron Sin (作物学报), 1998, 24(5): 550-557 (in Chinese with English abstract)
[33] Fehr W R, Caviness C E. Stages of Soybean Development. Agric Home Econ Exp Stn Spec Rep 80, Iowa State Univ, Ames, IA, USA, 1977
[34] Fei Z-H(费志宏), Wu C-X(吴存祥), Sun H-B(孙洪波), Hou W-S(侯文胜), Zhang B-S(张宝石), Han T-F(韩天富). Identification of photothermal responses in soybean by integrating photoperiod treatments with planting-date experiments. Acta Agron Sin (作物学报), 2009, 35(8): 1525-1531 (in Chinese with English abstract)
[35] Lander E S, Bostein D. Mapping Mendelian factors underlying quantitative traits using RFLP maps. Genetics, 1989, 121: 185-199
[36] Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122-128
[37] Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistastic effects and QTL environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255-1264
[38] Watanabe S, Hideshima R, Xia Z, Tsubokura Y, Sato S, NakamotoY,Yamanaka N, Takahashi R, Ishimoto M, Anai T, Tabata S, Harada K. Map-based cloning of the gene associated with soybean maturity locus E3. Genentics, 2009, 182: 1251-1262
[39] Kantolic A G, Slafer G A. Photoperiod sensitivity after flowering and seed number determination in indeterminate soybean cultivars. Field Crops Res, 2001, 72: 109-118
[40] Sinclair T R, Neumaier N, Farias J R B, Nepomuceno A L. Comparison of vegetative development in soybean cultivars for low-latitude environments. Field Crops Res, 2005, 92: 53-59
[41] Tomkins J P, Shipe E R. Environmental adaptation of long-juvenile soybean cultivars and elite strains. Agron J, 1997,89: 257-262
[42] Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000, 12: 2473-2483
[43] Sourdille P, Snape J W, Cadalen T, Charmet G, Nakata N, Bernard S, Bernard M. Detection of QTLs for heading time and photoperiod response in wheat using a doubled haploid population. Genome, 2000, 43: 487-494
[1] 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[2] 唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756.
[3] 郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657.
[4] 姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387.
[5] 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[6] 蒋嘉卉, 江炳志, 刘冠明, 王章英, 唐朝臣. 紫肉甘薯品质性状的近红外光谱预测模型构建与优化[J]. 作物学报, 2026, 52(4): 1088-1102.
[7] 刘长友, 王珅, 时会影, 沈颖超, 孙蕾, 王彦, 张志肖, 苏秋竹, 田静, 范保杰. 基于饭豆基因资源的小豆远缘杂交群体抗豆象QTL定位[J]. 作物学报, 2026, 52(3): 936-944.
[8] 李瑞, 余意雯, 王敦亮, 田婷, 孙灵湘, 陶玥玥, 孙华. 长江中下游油菜薹油兼用模式菜籽产量特征比较研究[J]. 作物学报, 2026, 52(2): 620-630.
[9] 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493.
[10] 杨锐, 陈敬东, 黄郢, 张学昆, 周登文, 刘清云, 徐劲松, 谢伶俐, 许本波. 基于北纬30°分界的长江中游油菜增产策略研究[J]. 作物学报, 2026, 52(1): 99-117.
[11] 迟晓元, 刘庆, 张君, 赵旭红, 李美, 于天一, 潘丽娟, 许静, 姜骁, 殷祥贞, 马俊卿, 陈娜. 不同花生品种(系)耐盐碱性田间鉴定及各性状指标相关性研究[J]. 作物学报, 2026, 52(1): 85-98.
[12] 王克晶, 李向华. 我国珍稀的大豆属多年生烟豆和短绒野大豆物种遗传资源濒危性评估分析[J]. 作物学报, 2025, 51(8): 2009-2019.
[13] 孟然, 李赵嘉, 冯薇, 陈悦, 刘路平, 杨春燕, 鲁雪林, 王秀萍. 大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J]. 作物学报, 2025, 51(8): 1991-2008.
[14] 贺红利, 张雨涵, 杨静, 程云清, 赵杨, 李星诺, 司洪亮, 张兴政, 杨向东. 大豆e1-as基因突变体的创制及生理分析[J]. 作物学报, 2025, 51(8): 2228-2239.
[15] 邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2