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作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1746-1753.doi: 10.3724/SP.J.1006.2013.01746

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

优良品系中品03-5373系谱的遗传解析及抗大豆胞囊线虫病相关标记鉴定

张姗姗1,**,李英慧1,**,李金英2,邱丽娟1,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程 / 农业部作物种质资源利用重点开放实验室，北京100081；2 河南省农业科学院经济作物研究所 / 国家大豆改良中心郑州分中心，河南郑州450002
  • 收稿日期:2013-01-28 修回日期:2013-05-11 出版日期:2013-10-12 网络出版日期:2013-08-01
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2010CB125903)和国家自然科学基金项目(31171575)资助。

Genetic Dissection of Elite Line Zhongpin 03-5373 Pedigree and Identification of Candidate Markers Related to Resistance to Soybean Cyst Nematode

ZHANG Shan-Shan1,**,LI Ying-Hui1,**,LI Jin-Ying2,QIU Li-Juan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Germplasm Utilization, Agriculture of Ministry/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Institute of Industrial Crops, Zhengzhou/ National Subcenter for Soybean Improvement, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2013-01-28 Revised:2013-05-11 Published:2013-10-12 Published online:2013-08-01
  • Contact: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843

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摘要：

中品03-5373是高抗大豆胞囊线虫(soybean cyst nematode，SCN) 3号生理小种的优良大豆新种质，可追溯到10个祖先亲本，其中包括灰皮支黑豆、Peking和PI437654等国内外SCN主要抗源。本研究利用152个SSR标记对中品03-5373及其亲本进行鉴定，共发现等位变异437个，每个标记的等位变异范围为2~5个，平均为2.9个。亲缘关系分析表明11份材料间的遗传一致度变化范围为0.2430~0.8224，平均为0.458，4个SSR标记(Satt152、Satt179、Barcsoyssr_18_107和Satt196)形成的单倍型可以将11份材料区别开来。系谱追踪阐明了育种对基因组组成变化的作用，发现中品03-5373亲本中以灰皮支黑豆贡献的等位变异最多(39个)，PI437654次之(6个)。通过系谱追踪筛选到与SCN 3抗性相关的候选标记20个，为进一步克隆抗病基因和选择有效的标记组合进行分子育种提供了依据。

关键词: 大豆, 胞囊线虫病, 系谱分析, 分子标记

Abstract:

Zhongpin 03-5373 with resistance to SCN3 is an elite soybean line, which is traced back to 10 parental lines. Three elite resistant sources (Huipizhiheidou, Peking and PI437654), diversely used in the soybean cyst nematode resistant breeding, were included in the pedigree of Zhongpin 03-5373. In this study, 152 molecular markers were selected to detect polymorphism among the 11 materials. A total of 437 alleles were identified with average of 2.9, ranging from two to five alleles on each locus. The average of Nei’s genetic identity among pairwise cultivars were 0.458, the haplotypes formed by four SSR markers (Satt152, Satt179, Barcsoyssr_18_107, and Satt196) could distinguish 11 cultivars in this study. Pedigree tracing elucidated genomic variation in breeding. IBD analysis showed that Huipizhiheidou contributed the most of specific alleles (39) to Zhongpin 03-5373 among three resistant genetic resources (Huipizhiheidou, Peking, and PI437654), followed by PI437654 (6). In addition, 20 markers identified were related to the resistance to SCN 3. These results supplied the information for further cloning resistant gene and resistant breeding by marker assistant selection.

Key words: Soybean, Soybean cyst nematode, Pedigree analysis, Molecular marker

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