作物学报 ›› 2016, Vol. 42 ›› Issue (07): 1000-1008.doi: 10.3724/SP.J.1006.2016.01000
刘睿洋,刘芳,官春云
LIU Rui-Yang,LIU Fang,GUAN Chun-Yun
摘要:
高油酸油具备较高的营养价值,在甘蓝型油菜中,脂肪酸去饱和酶基因(FAD2)是控制油酸含量的关键基因。本研究克隆了甘蓝型油菜A5、C5、A1连锁群上3个BnFAD2基因的全长cDNA序列,分别命名为BnFAD2-A5、BnFAD2-C5和BnFAD2-A1,各自编码384、384、136个氨基酸。分别使用TMHMM、Clust X软件分析FAD2基因的跨膜结构域和酶活中心表明,BnFAD2-A1不具备脱氢酶活性。采用酵母功能互补实验对4个基因(含已发表的BnFAD2-C1)进行功能验证,发现BnFAD2-A5和BnFAD2-C5基因去饱和能力接近,均大于BnFAD2-C1基因。采用qRT-PCR分析4个基因在甘蓝型油菜不同组织中的表达规律及血凝素标签法分析BnFAD2-C1、BnFAD2-A5和BnFAD2-C5的蛋白稳定性,表明BnFAD2-A5和BnFAD2-C5是影响油菜种子油酸积累的主效基因。
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