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作物学报 ›› 2017, Vol. 43 ›› Issue (03): 315-323.doi: 10.3724/SP.J.1006.2017.00315

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

OsWR2-RNAi对水稻角质层生物合成和耐旱性的影响

王莎1,贺勇1,罗光宇1,姚敏1,张旭1,陈信波1,2,周小云1,2,*   

  1. 1湖南农业大学生物科学技术学院,湖南长沙 410128;2作物基因工程湖南省重点实验室,湖南长沙 410128
  • 收稿日期:2016-07-20 修回日期:2016-11-02 出版日期:2017-03-12 网络出版日期:2016-11-11
  • 通讯作者: 周小云, E-mail: xyzhou71@hotmail.com
  • 基金资助:

    本研究由湖南省重点研发计划项目(2015JC3102), 湖南农业大学大学生创新计划项目(XCX1504)和国家自然科学基金项目(31300216)资助。

Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance

WANG Sha1,HE Yong1,LUO Guang-Yu1,YAO Min1,ZHANG Xu1,CHEN Xin-Bo1,2,ZHOU Xiao-Yun1,2,*   

  1. 1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; 2 Crop Gene Engineering Key Laboratory of Hunan Province, Changsha 410128, China
  • Received:2016-07-20 Revised:2016-11-02 Published:2017-03-12 Published online:2016-11-11
  • Contact: 周小云, E-mail: xyzhou71@hotmail.com
  • Supported by:

    This study was supported by the Key Research Projects of Hunan (2015JC3102), the College Students Innovation Project of Hunan Agricultural University (XCX1504), and the National Natural Science Foundation of China (31300216).

摘要:

植物的角质层在干旱胁迫下对降低植物的非气孔性失水起重要作用。对水稻蜡质相关转录因子基因OsWR2过表达研究发现,OsWR2对水稻表皮蜡质和角质的含量和组分构成以及非气孔性失水都有影响,推测OsWR2是植物角质层代谢途径中重要的调控基因。本研究通过构建OsWR2-RNAi载体并转入水稻获得抑制OsWR2表达的突变转基因植株,发现OsWR2-RNAi水稻叶片角质层组成和含量明显变化,其蜡质组分中醛类、醇类和烷烃类的减少导致表皮蜡质晶体的积累和表皮蜡质总量减少14.8%,角质单体中C16:0和C18:1ω-OH脂肪酸和di-OH脂肪酸等角质组分含量的显著降低导致OsWR2-RNAi水稻叶片中角质单体总量减少36.2%。幼苗干旱处理前后,OsWR2-RNAi植株自由脯氨酸含量与野生型相比变化不大,但其表皮通透性、水分散失率和MAD含量显著增加,幼苗耐旱性降低。本研究结果进一步证实OSWR2对水稻蜡质和角质生物合成及对非气孔性失水的调控作用,有望为水稻耐旱性状的改良提供重要基因资源。

关键词: OsWR2, RNAi, 植物角质层, 干旱胁迫, 非气孔性失水

Abstract:

Plant cuticle plays an important role on decreasing plant non-stomatal water loss under drought stress. Overexpression of wax related transcription factor gene OsWR2 in rice can increase total cuticular wax and cutin amounts, affect cuticular wax and cutin monomer composition, and alter cuticular wax crystallization patterns and cuticle membrane ultrastructure, implying important roles of OsWR2 in rice cuticle biosynthesis and non-stomatal water loss regulation. In this report, we constructed OsWR2 RNA inhibition vector (OsWR2-RNAi), and transformed it into rice callus to obtain OsWR2 knockout rice mutants using Agrobacterium-mediated transforming method. OsWR2-RNAi transgenic rice exhibited reduced total cuticular wax amounts by 14.8% mainly due to the decrease of aldehydes, alkanes and alcohols, and lessened full cutin monomer level by 36.2% due primarily to the decrease of C16:0, C18:1 ω-OH and di-OH fatty acid components. OsWR2-RNAi transgenic rice also showed enriched leaf chlorophyll leaching, water loss rates and MDA contents, whereas reduced tolerance to water deficit . All these physiologic and biochemical index to support the previous hypothesis ofOsWR2 acting as a transcriptional regulator of both cuticle biosynthetic pathways and non-stomatal water loss, providing evidence of OsWR2 exerting direct influence over rice dehydration, and a potential application resource in genetic improvement of crop drought tolerance.

Key words: OsWR2, RNAi, Plant cuticle, Drought stress, Non-stomatal water loss

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