欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2019, Vol. 45 ›› Issue (7): 1090-1098.doi: 10.3724/SP.J.1006.2019.84134

• 耕作栽培·生理生化 • 上一篇    下一篇

晾制密度对雪茄烟叶膜脂过氧化作用及品质的影响

赵松超,李一凡,刘博远,赵铭钦()   

  1. 河南农业大学烟草学院, 河南郑州 450000
  • 收稿日期:2018-10-26 接受日期:2019-01-12 出版日期:2019-07-12 网络出版日期:2019-02-21
  • 通讯作者: 赵铭钦
  • 作者简介:E-mail: zhaosongchao1314@126.com
  • 基金资助:
    本研究由中国烟草总公司海南省公司科技重点项目“优质雪茄烟叶晾房研制及晾制技术研究”(201746000024054)

Effects of air drying density on membranous lipid peroxidation and quality of cigar tobacco leaf

ZHAO Song-Chao,LI Yi-Fan,LIU Bo-Yuan,ZHAO Ming-Qin()   

  1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450000, Henan, China
  • Received:2018-10-26 Accepted:2019-01-12 Published:2019-07-12 Published online:2019-02-21
  • Contact: Ming-Qin ZHAO
  • Supported by:
    This study was supported by the Key Scientific and Technological Project of Hainan Province, China Tobacco Corporation: “Research of Quality Cigar Tobacco Leaf Drying Room and Research of Drying Technology”(201746000024054)

RichHTML

4

PDF

503

摘要：

为了解不同晾制密度条件下膜脂过氧化水平对雪茄烟叶品质的影响, 本文设计了杆距10 cm、杆距20 cm、杆距30 cm 3种晾制密度, 研究其对雪茄烟叶所在晾房的温湿度、烟叶含水率、丙二醛含量、抗氧化酶活性、多酚和化学成分的影响。结果表明, 杆距20 cm的晾制密度过氧化水平最低, 且与晾房内温湿度相关性不显著, 只与含水率相关, 说明该晾制密度较其他晾制密度有利于避免膜脂过氧化水平的升高; 烟叶中过氧化物酶、超氧化物歧化酶、过氧化氢酶和多酚含量均在杆距20 cm晾制密度下最高, 有利于增强烟叶的抗氧化能力; 多酚氧化酶活性在杆距10 cm晾制密度下最高; 化学成分以杆距20 cm处理较为协调。因此杆距为20 cm的晾制密度有利于提高抗氧化类物质, 减轻膜脂过氧化水平的伤害, 提升烟叶品质。

关键词: 晾制密度, 膜脂过氧化水平, 温湿度, 含水率, 酶活性, 多酚, 化学成分

Abstract:

In order to understand the influence of different drying density on membranous lipid peroxidation level and cigar tobacco leaf quality, an experiment was conducted with three treatments (stem distance of 10 cm, stem distance of 20 cm, stem distance of 30 cm) of drying system to measure the temperature and humidity of drying room, tobacco leaf water content, malondialdehyde (MDA) content, antioxidant enzyme activity, polyphenol and the chemical compositions. The level of peroxidation in the treatment of 20 cm was the lowest, and had no significant correlation with the temperature and humidity in the drying room, but correlated with the water content. The activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and polyphenol content in leaves were the highest under the drying density of 20 cm, indicating that the drying density of 20 cm was conducive to the improvement of antioxidant enzyme activity, polyphenol content, and antioxidant capacity of tobacco leaves. The activity of polyphenol oxidase (PPO) was highest at the drying density of 10 cm and the chemical components were coordinates in the treatment of 20 cm. Therefore, the air-curing density with a length of 20 cm is conducive to increasing antioxidants, reducing the damage caused by membranous lipid peroxidation and improving the quality of tobacco leaves.

Key words: air drying density, lipid peroxidation level, temperature and humidity, moisture content, enzyme activity, polyphenols, chemical composition

表1

晾制密度对晾房内温湿度和烟叶含水率的影响"

测定指标
Index		 处理
Treatment		 处理后天数 Days after treatment
0 d 5 d 10 d 15 d 20 d 25 d
温度
Air temperature
(℃)		 杆距为10 cm的高晾制密度 T1 26.64 a 33.46 a 34.22 a 38.86 a 39.77 a 38.18 a
杆距为20 cm的中晾制密度 T2 25.08 a 32.24 b 33.16 b 37.36 b 38.98 ab 38.43 a
杆距为30 cm的低晾制密度 T3 24.18 a 31.8 b 31.72 c 36.34 c 38.42 b 36.81 a
相对湿度
Relative humidity (%)		 杆距为10 cm的高晾制密度 T1 69.86 a 87.66 a 89.12 a 74.23 a 68.39 a 61.87 a
杆距为20 cm的中晾制密度 T2 70.14 a 82.18 b 84.05 b 66.35 b 56.34 b 56.46 a
杆距为30 cm的低晾制密度 T3 70.18 a 75.32 c 79.16 c 58.92 c 52.64 b 52.68 a
含水率
Moisture content (%)		 杆距为10 cm的高晾制密度 T1 89.4 a 83.1 a 74.2 a 36.8 a 24.9 a 16.4 a
杆距为20 cm的中晾制密度 T2 90.2 a 76.3 b 61.9 b 27.2 b 16.6 b 10.3 b
杆距为30 cm的低晾制密度 T3 90.5 a 69.2 c 48.3 c 17.3 c 11.9 c 9.1 b

图1

晾制密度对MDA含量的影响 处理同表1。"

表2

温湿度和烟叶水分与膜脂过氧化水平的相关系数"

处理
Treatment		 温度
Air temperature		 相对湿度
Relative humidity		 含水率
Moisture content
杆距为10 cm的高晾制密度T1 0.817* -0.659 -0.913*
杆距为20 cm的中晾制密度T2 0.771 -0.790 -0.893*
杆距为30 cm的低晾制密度T3 0.810 -0.827* -0.917**

图2

不同晾制密度的雪茄烟叶POD (A)、CAT (B)、SOD (C)活性 处理同表1。"

图3

不同晾制密度对烟叶多酚氧化酶活性的影响 处理同表1。"

图4

不同晾制密度对绿原酸(A)和芸香苷(B)含量的影响 处理同表1。"

表3

晾制密度对雪茄烟叶化学成分的影响"

处理
Treatment		 化学成分 Chemical composition
总糖
Total sugar
(%)		 烟碱
Nicotine
(%)		 淀粉
Starch
(%)		 钾
Potassium
(%)		 氯
Chlorin
(%)		 还原糖
Reducing sugar (%)		 两糖比
Two sugar ratio		 钾氯比
Potassium/
chloride ratio
T1 1.78 b 2.95 a 1.74 a 4.08 a 0.73 a 1.18 b 0.66 b 5.59 b
T2 1.95 a 2.81 a 1.04 b 4.23 a 0.68 a 1.41 a 0.72 a 6.22 a
T3 1.75 b 2.61 a 1.95 a 4.14 a 0.70 a 1.02 b 0.58 b 5.92 ab
[1] 邵雨, 符雷 . 海南雪茄烟叶发展存在的问题及对策. 见: 烟草学会主编. 中国烟草学会2015年度优秀论文汇编. 北京: 中国烟草学会, 2015. p 4.
Shao Y, Fu L. Problems and countermeasures of cigar tobacco leaf development in Hainan province. In: Tobacco Society, eds. Annual Excellent Paper Collection, China Tobacco Society, 2015. Beijing: China Tobacco Society, 2015. p 4 (in Chinese).
[2] 王浩雅, 左兴俊, 孙福山, 王传义, 王松峰, 王爱华, 王晓宾, 程浩, 温亮, 刘勇, 贾虎虎 . 雪茄烟外包叶的研究进展. 中国烟草科学, 2009,30(5):71-76.
Wang H Y, Zuo X J, Sun F S, Wang C Y, Wang S F, Wang A H, Wang X B, Cheng H, Wen L, Liu Y, Jia H H . Advance in cigar wrapper tobacco. China Tob Sci, 2009,30(5):71-76 (in Chinese with English abstract).
[3] 韩锦峰, 汪耀富, 林学梧, 郑英, 王子杰 . 烤烟叶片成熟度与细胞膜脂过氧化及体内保护酶活性关系的研究. 中国烟草学报, 1994,2(1):20-24.
Han J F, Wang Y F, Lin X W, Zheng Y, Wang Z J . Studies on the cell membrane-lipid peroxidation and endogenous protective enzymes activity in relation to leaf maturity of flue-cured tobacco. Acta Tob Sin, 1994,2(1):20-24 (in Chinese with English abstract).
[4] 宋凤鸣, 葛秀春, 郑重 . 活性氧及膜脂过氧化与棉花对枯萎病抗性的关系. 植物病理学报, 2001,31:110-116.
Song F M, Ge X C, Zheng Z . The roles of active oxygen species and lipid peroxidation in the resistance of cotton seedlings to fusarium wilt. Acta Phytopathol Sin, 2001,31:110-116 (in Chinese with English abstract).
[5] 陈秋芳, 郭月清 . 白肋烟调制过程中叶片膜脂过氧化特性的研究. 河南农业大学学报, 1997,31(4):24-27.
Chen Q F, Guo Y Q . The characteristics of membrane lipid peroxidation in burley tobacco leaves during curing. J Henan Agric Univ, 1997,31(4):24-27 (in Chinese with English abstract).
[6] 黄山, 杨虹琦, 张发明, 李自相, 岳骞, 陈文俊 . 烘烤温湿度变化对不同烤烟品种烟叶膜脂过氧化作用的影响. 湖南农业大学学报(自科版), 2009,35:485-492.
Huang S, Yang H Q, Zhang F M, Li Z X, Yue S, Chen W J . Effect of baking temperature and humidity change on leaf membrane lipid peroxidation to the different varieties of flue-cured tobacco. J Hunan Agric Univ(Nat Sci Edn), 2009,35:485-492 (in Chinese with English abstract).
[7] 杨虹琦 . 烘烤温湿度变化对不同烤烟品种烟叶膜脂过氧化作用的影响. 见: 中国烟草学会2009年年会论文集. 北京: 中国烟草学会, 2009. p 7.
Yang H Q. Effects of baking temperature and humidity on membrane lipid peroxidation of different tobacco varieties. In: Proceedings of the Annual Meeting of China Tobacco Society 2009. Beijing: China Tobacco Society Press, 2009. p 7 (in Chinese).
[8] 王卫峰, 王松峰, 陈江华, 王爱华, 毋丽丽, 黄永城, 景延秋, 宫长荣 . 装烟密度对烟叶烘烤过程中几种抗氧化酶活性的影响. 植物生理学通讯, 2006,42:817-820.
Wang W F, Wang S F, Chen J H, Wang A H, Wu L L, Huang Y C, Jing Y Q, Gong C R . Effects of loading density on the activities of several antioxidant enzymes of tobacco (Nicotiana tabacum L.) leaves in bulk curing barn. Plant Physiol Commun, 2006,42:817-820 (in Chinese with English abstract).
[9] 汪耀富, 宫长荣, 赵铭钦, 赵会杰, 孙德梅 . 烤烟烘烤过程中叶片膜脂过氧化特性的研究. 河南农业大学学报, 1995,29:247-250.
Wang Y F, Gong C R, Zhao M Q, Zhao H J, Sun D M . The characteristics of membrane lipid peroxidation in tobacco leaves during flue curing. J Henan Agric Univ, 1995,29:247-250 (in Chinese with English abstract).
[10] 李璇 . 环境胁迫下植物抗氧化酶的反应规律研究. 见: 中国药学会主编. 2010年中国药学大会暨第十届中国药师周论文集, 2010. p 8.
Li X. Study on the response of plant antioxidant enzymes under environmental stress. In: China Pharmaceutical Congress and the 10th China Pharmacist Week Symposium, 2010. p 8 (in Chinese).
[11] 黄莉, 丁波 . 植物多酚的抗氧化机理及其在动物生产中的应用. 中国畜牧兽医, 2018,45:2471-2477.
Huang L, Ding B . Antioxidant mechanism of plant polyphenols and its application in animal production. China Anim Husb Vet Med, 2018,45:2471-2477 (in Chinese with English abstract).
[12] 陈仁霄, 何宽信, 李立新, 冯小虎, 潘日洪, 王利兵, 陈衍泽 . 气流下降式密集烤房不同装烟密度对烟叶烘烤效果影响. 江西农业大学学报, 2014,36:272-278.
Chen R X, He K X, Li L X, Feng X H, Pan R H, Wang L B, Chen Y Z . Effects of loading density on quality of tobacco leaves under the condition of down-draft bulk curing barn. Acta Agric Univ Jiangxiensis, 2014,36:272-278 (in Chinese with English abstract).
[13] 张滔 . 密集烤房装烟密度对烟叶质量的影响. 湖南农业大学硕士学位论文, 湖南长沙, 2013.
Zhang T . The effect of loading density in bulk curing barn on tobacco leaf quality. MS Thesis of Hunan Agricultural University, Changsha, China, 2013 (in Chinese with English abstract).
[14] 贺帆, 王涛, 樊军辉, 杨彦明, 刘晓迪, 宫长荣 . 装烟密度对密集烘烤中上部烟叶香气质量的影响. 江西农业大学学报, 2013,35(1):33-37.
He F, Wang T, Fan J H, Yang Y M, Liu X D, Gong C R . Effects of loading density of bulk curing barn on aroma components of flue-cured lumbar and upper tobacco leaves. Acta Agric Univ Jiangxiensis, 2013,35(1):33-37 (in Chinese with English abstract).
[15] 张志良 . 植物生理学实验指导(第2版). 北京: 高等教育出版社, 1990. pp 1-2.
Zhang Z L. Experimental Instruction in Plant Physiology (2nd edn). Beijing: Higher Education Press, 1990. pp 1-2(in Chinese).
[16] 郝再彬 . 植物生理实验. 哈尔滨: 哈尔滨工业大学出版社, 2004. pp 106-116.
Hao Z B . Plant Physiology Experiment. Harbin: Harbin Industrial University Press, 2004. pp 106-116(in Chinese).
[17] 徐朗莱, 叶茂炳 . 过氧化物酶活力连续记录测定法. 南京农业大学学报, 1989,12(3):82-83.
Xu L L, Ye M B . Continuous recording of peroxidase activity. J Nanjing Agric Univ, 1989,12(3):82-83 (in Chinese with English abstract).
[18] 李仕飞, 刘世同, 周建平, 罗天雄 . 分光光度法测定植物过氧化氢酶活性的研究. 安徽农学通报, 2007,13(2):72-73.
Li S F, Liu S T, Zhou J P, Luo T X . Spectrophotometric determination of catalase activity in plants. Anhui Agric Bull, 2007,13(2):72-73 (in Chinese).
[19] 余小芬, 谢燕, 郑波, 彭荣珍, 付斌, 邱学礼 . 高效液相色谱法测定烟草中多酚类物质. 西南农业学报, 2013,26:531-534.
Yu X F, Xie Y, Zheng B, Peng R Z, Fu B, Qiu X L . HPLC determination of polyphenols in tobacco. Southwest China J Agric Sci, 2013,26:531-534 (in Chinese with English abstract).
[20] 程传玲, 唐琦, 汪文良, 刘艳芳, 王建民 . 烤烟常规化学成分与感官质量的典型相关分析. 贵州农业科学, 2011,39(1):59-61.
Cheng C L, Tang Q, Wang W L, Liu Y F, Wang J M . Canonical correlation between conventional chemical composition and sensory quality in flue-cured tobacco. Guizhou Agric Sci, 2011,39(1):59-61 (in Chinese with English abstract).
[21] 张宇婷, 高建民, 张琼琳, 张爱东, 王海霞, 孙杰 . 植物超氧化物歧化酶的研究进展. 畜牧与饲料科学, 2016,37(9):28-31.
Zhang Y T, Gao J M, Zhang Q L, Zhang A D, Wang H X, Sun J . Research progress on plant superoxide dismutase. Anim Husb Feed Sci, 2016,37(9):28-31 (in Chinese).
[22] 李丛民 . 植物多酚对烟草制品品质的影响. 烟草科技, 2000, ( 1):27-28.
Li C M . Effects of plant polyphenols on the quality of tobacco products. Tob Sci Technol, 2000, ( 1):27-28 (in Chinese).
[23] 刘国顺 . 烟草栽培学. 北京: 中国农业出版社, 2003. pp 72-74.
Liu G S . Tobacco Cultivation. Beijing: China Agriculture Press, 2003. pp 72-74(in Chinese).
[24] 王勇, 王行, 贺广生, 王军, 罗宇芬, 陆丽, 彭桂香, 谭志远 . 耐高温产淀粉酶芽孢杆菌在烟叶烘烤中降解淀粉的应用研究. 中国烟草学报, 2017,23(4):56-63.
Wang Y, Wang X, He G S, Wang J, Luo Y F, Lu L, Peng G X, Tan Z Y . Effects of amylase producing thermophilic Bacillus strains on starch degradation in tobacco during flue-curing. Acta Tob Sin, 2017,23(4):56-63 (in Chinese with English abstract).
[25] 常剑波, 王勇军, 祁春苗, 王江朝, 朱顺成, 关卫东, 邢云霞 . 不同装烟密度和风机配置对密集烤房内叶间隙风速和烤后烟外观质量的影响. 现代化农业, 2017, ( 1):29-32.
Chang J B, Wang Y J, Qi C M, Wang J C, Zhu S C, Guan W D, Xing Y X . The effects of different densities and fan configurations on the air velocity in the interleaf gap and the smoke appearance quality after baking. Modern Agric, 2017, ( 1):29-32 (in Chinese).
[26] 任彩虹, 闫桂琴, 郜刚, 张丽萍 . 高温胁迫对马铃薯幼苗叶片生理效应的影响. 中国马铃薯, 2007,21(1):5-10.
Ren C H, Yan G Q, Gao G, Zhang L P . Effects of high temperature stress on physiological effects of potato seedling leaves. China Potato J, 2007,21(1):5-10 (in Chinese with English abstract).
[27] 宫长荣, 李艳梅, 李常军 . 烘烤过程中烟叶脂氧合酶活性与膜脂过氧化的关系. 中国烟草学报, 2000,6(1):39-41.
Gong C R, Li Y M, Li C J . Relationship between lox activity and lipid peroxidation in tobacco leaf. Acta Tob Sin, 2000,6(1):39-41 (in Chinese with English abstract).
[28] 朱雪梅, 柯永培, 邵继荣, 林立金, 杨远祥 . 高温胁迫对重穗型水稻品种叶片活性氧代谢的影响. 种子, 2005,24(3):25-27.
Zhu X M, Ke Y P, Shao J R, Lin L J, Yang Y X . Effects of high temperature stress on activated oxygen metabolism of the leaves of the heavy panicle type of medium India hybrid rice. Seed, 2005,24(3):25-27 (in Chinese).
[29] Lee B R, Kim K Y, Jung W J, Avice J C, Ourry A, Kim T H . Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.). J Exp Bot, 2007,58:1271-1279.
[30] Sofo A, Dichio B, Xiloyannis C, Masia A . Antioxidant defences in olive trees during drought stress: changes in activity of some antioxidant enzymes. Funct Plant Biol, 2005,32:351-364.
[31] 朱大恒, 张闪闪, 莫骏坚, 朱润琪, 汪耀富, 刘丽, 马轩, 席宇 . 光强对湘南烟草生育后期光合及生理特性的影响. 江苏农业科学, 2018,46(8):81-84.
Zhu D H, Zhang S S, Mo J J, Zhu R Q, Wang Y F, Liu L, Ma X, Xi Y . Effects of light intensity on photosynthesis and physiological characteristics of tobacco in late growth period in southern Hunan. Jiangsu Agric Sci, 2018,46(8):81-84 (in Chinese with English abstract).
[32] 兰俊荣, 靖军领, 黄一兰, 彭怀俊 . 不同成熟度烟叶烘烤过程中多酚氧化酶活性变化. 现代农业科技, 2010, ( 23):54-55.
Lan J R, Jing J L, Huang Y L, Peng H J . Changes of polyphenol oxidase activity in different maturity tobacco during flue-curing process. Modern Agric Sci Technol, 2010, ( 23):54-55 (in Chinese).
[33] 寇明钰, 李宁, 吴艳 . 棕色化反应调控技术在烟草中的应用现状及展望. 湖北农业科学, 2009,48:2013-2016.
Kou M Y, Li N, Wu Y . The application and prospect of controlling techniques of tobacco browning reaction. Hubei Agric Sci, 2009,48:2013-2016 (in Chinese with English abstract).
[34] 周冀衡 . 烟草生理与生物化学. 合肥: 中国科学技术大学出版社, 1996. pp 458-461.
Zhou J H. Tobacco Physiology and Biochemistry. Hefei: Press of University of Science and Technology of China, 1996. pp 458-461(in Chinese).
[35] 宋洋洋, 张小全, 杨铁钊, 彭玉富, 王冬, 武云杰, 姚健, 焦绍赫 . 烟叶采收成熟度对烘烤过程中酶促棕色化反应相关指标的影响. 西北植物学报, 2014,34:2459-2466.
Song Y Y, Zhang X Q, Yang T Z, Peng Y F, Wang D, Wu Y J, Yao J, Jiao S H . Effect of different harvesting maturities of flue-cured tobacco on related indicators in enzymatic browning reaction during curing. Acta Bot Boreali-Occident Sin, 2014,34:2459-2466 (in Chinese with English abstract).
[36] 宋朝鹏 . 白肋烟晾制过程中主要生理变化和收晾处理对品质的影响. 河南农业大学硕士学位论文,河南郑州, 2004.
Song C P . The Change of Major Physiology and the Influence of Different Harvesting Treatments on Burley Tobacco Quality during Air-curing. MS Thesis of Henan Agriculture University, Zhengzhou, Henan, China, 2004 (in Chinese).
[37] 李秀妮, 乔保民, 吴创, 田煜利, 万德建, 时向东 . 晾晒烟调制过程中酶促棕色化反应及调控方法. 天津农业科学, 2018, ( 2):76-79.
Li X N, Qiao B M, Wu C, Tian Y L, Wan D J, Shi X D . Enzymatic browning reaction and its regulation method during the process of sun/air-cured tobacco. Tianjin Agric Sci, 2018, ( 2):76-79 (in Chinese).
[38] 韩锦峰 . 烟草栽培生理. 北京: 中国农业出版社, 2003. pp 199-202.
Han J F. Physiology of Tobacco Cultivation. Beijing: China Agriculture Press, 2003. pp 199-202(in Chinese).
[39] 李昊阳, 夏继桥, 杨连玉, 钟荣珍 . 植物多酚的抗氧化能力及其在动物生产中的应用. 动物营养学报, 2013,25:2529-2534.
Li H Y, Xia J Q, Yang L Y, Zhong R Z . Plant polyphenols: antioxidant capacity and application in animal production. Chin J Anim Nutr, 2013,25:2529-2534 (in Chinese with English abstract).
[40] 宫长荣, 王爱华, 王松峰 . 烟叶烘烤过程中多酚类物质的变化及与化学成分的相关分析. 中国农业科学, 2005,38:2316-2320.
Gong C R, Wang A H, Wang S F . Changes of polyphenols in tobacco leaves during the flue-curing process and relative analysis of some chemical components. Sci Agric Sin, 2005,38:2316-2320 (in Chinese with English abstract).
[41] 孙达旺 . 植物单宁化学. 北京: 中国林业出版社, 1992. p 66.
Sun D W . Plant Tannin Chemistry. Beijing: China Forestry Publishing House, 1992. p 66 (in Chinese).
[42] 中国科学院北京植物研究所鸭梨黑心病研究小组. 鸭梨黑心病的研究: II. 酚类物质的酶促褐变. 植物学报, 1974,16:236-242.
Research Group for Brown Core of Pear, Peking Institute of Botany, CAS. Studies on the brown core of pear fruit: II. The enzymatic browning of polyphenolic acid during storage. Acta Bot Sin, 1974,16:236-242 (in Chinese with English abstract).
[43] 宫长荣, 陈江华, 汪耀富 . 烘烤中的膜脂过氧化作用及其对烟叶内在质量的影响. 中国烟草学报, 1999,5(3):11-16.
Gong C R, Chen J H, Wang Y F . Characteristics of membrane lipid peroxidation in tobacco leaves during curing and its effect on quality. Acta Tob Sin, 1999,5(3):11-16 (in Chinese).
[44] 王怀珠, 杨焕文, 郭红英 . 烘烤过程中不同成熟度烟叶淀粉的降解动态. 烟草科技, 2004, ( 10):36-39.
Wang H Z, Yang H W, Guo H Y . Studies on starch degradation in tobacco leaves of different maturity during flue-curing. Tob Sci Technol, 2004, ( 10):36-39 (in Chinese).
[45] Weeks W W . Chemistry of tobacco constituents influences flavor and aroma. Rec Adv Tob Sci, 1985,11:175-200.
[1] 黄义文, 代旭冉, 刘宏伟, 杨丽, 买春艳, 于立强, 于广军, 张宏军, 李洪杰, 周阳. 小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系[J]. 作物学报, 2021, 47(11): 2080-2090.
[2] 李璐璐, 明博, 初振东, 张万旭, 高尚, 王浥州, 侯梁宇, 周先林, 谢瑞芝, 王克如, 侯鹏, 李少昆. 适宜机械粒收玉米品种的熟期评价指标[J]. 作物学报, 2021, 47(11): 2199-2207.
[3] 陈明俊,舒启琼,徐建飞,罗小波,雷尊国,金黎平,李飞. 抗褐变马铃薯品种(系)鉴定与筛选[J]. 作物学报, 2020, 46(8): 1208-1216.
[4] 赵波,李小龙,周茂林,宋碧,雷恩,李钟,吴雅薇,袁继超,孔凡磊. 西南玉米机械粒收籽粒破碎率现状及影响因素分析[J]. 作物学报, 2020, 46(01): 74-83.
[5] 要凯,赵章平,康益晨,张卫娜,石铭福,杨昕宇,范艳玲,秦舒浩. 沟垄覆膜对连作马铃薯土壤酶活性、理化性状及产量的影响[J]. 作物学报, 2019, 45(8): 1286-1292.
[6] 王凯,赵小红,姚晓华,姚有华,白羿雄,吴昆仑. 茎秆特性和木质素合成与青稞抗倒伏关系[J]. 作物学报, 2019, 45(4): 621-627.
[7] 孙凯,刘振,胡恒宇,李耕,刘文涛,杨柳,宁堂原,王彦玲. 有机培肥与轮耕方式对夏玉米田土壤碳氮和产量的影响[J]. 作物学报, 2019, 45(3): 401-410.
[8] 樊海潮,顾万荣,杨德光,尉菊萍,朴琳,张倩,张立国,杨秀红,魏湜. 化控剂对东北春玉米茎秆理化特性及抗倒伏的影响[J]. 作物学报, 2018, 44(6): 909-919.
[9] 李璐璐,明博,谢瑞芝,王克如,侯鹏,李少昆. 黄淮海夏玉米品种脱水类型与机械粒收时间的确立[J]. 作物学报, 2018, 44(12): 1764-1773.
[10] 薛军,王群,李璐璐,张万旭,谢瑞芝,王克如,明博,侯鹏,李少昆. 玉米生理成熟后倒伏变化及其影响因素[J]. 作物学报, 2018, 44(12): 1782-1792.
[11] 高尚,明博,李璐璐,谢瑞芝,薛军,侯鹏,王克如,李少昆. 黄淮海夏玉米籽粒脱水与气象因子的关系[J]. 作物学报, 2018, 44(12): 1755-1763.
[12] 胡一晨,赵钢,秦培友,成颜芬,曹亚楠,邹亮,任贵兴. 藜麦活性成分研究进展[J]. 作物学报, 2018, 44(11): 1579-1591.
[13] 方彦，孙万仓，武军艳，刘自刚，董云，米超，马骊，陈奇，何辉立. 北方白菜型冬油菜的膜脂脂肪酸组分和ATPase活性对温度的响应[J]. 作物学报, 2018, 44(01): 95-104.
[14] 陈鸿飞,庞晓敏,张仁,张志兴,徐倩华,方长旬,李经勇,林文雄. 不同水肥运筹对再生季稻根际土壤酶活性及微生物功能多样性的影响[J]. 作物学报, 2017, 43(10): 1507-1517.
[15] 陈金,庞党伟,韩明明,尹燕枰,郑孟静,骆永丽,王振林*,李勇*. 耕作模式对土壤生物活性与养分有效性及冬小麦产量的影响[J]. 作物学报, 2017, 43(08): 1245-1253.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2