作物学报 ›› 2023, Vol. 49 ›› Issue (9): 2582-2593.doi: 10.3724/SP.J.1006.2023.24195
苏一钧1(), 赵路宽2, 唐芬3, 戴习彬1, 孙亚伟1, 周志林1, 刘亚菊1, 曹清河1,2,3,*()
SU Yi-Jun1(), ZHAO Lu-Kuan2, TANG Fen3, DAI Xi-Bin1, SUN Ya-Wei1, ZHOU Zhi-Lin1, LIU Ya-Ju1, CAO Qing-He1,2,3,*()
摘要:
甘薯起源于中南美洲, 国外引进种不仅能够提升我国资源总量而且将会极大改变甘薯遗传基础狭窄的育种瓶颈。本文利用30对Simple sequence repeat (SSR)引物通过高通量核酸片段分析仪对378份甘薯种质资源进行基因分型, 鉴定了国家甘薯种质资源试管苗库(徐州)中的引进种种质, 分析了引进种之间的亲缘关系, 为甘薯引进种的利用和种质创新提供理论依据。利用PROSize 3.0软件读取条带, 获得01数据库, 将01数据导入Darwin软件计算Jaccard遗传距离矩阵, 利用MEGA 10对遗传距离矩阵聚类分析, 并用iTOL进行注释, 利用Structure 2.3.4对甘薯种质进行群体结构分析。结果表明, 对378份引进种进行分析, 共扩增获得120个多态性位点, 平均每对引物4个多态性位点。遗传距离矩阵结果显示种质间遗传距离最大为0.6207, 遗传距离最小为0.0448, 平均遗传距离为0.4027。利用MEGA 10进行聚类, 在遗传距离为0.2960处划分为3个类群, 类群I包含29份种质、类群II包含104份种质、类群III包含245份种质; 群体结构分析表明在ΔK=2时值最大, 划分为2个组群, 聚类与群体结构分析结果类似。组群2中, 主要以2015年以后新引进种质为主(95.4%), 表明新引进种质丰富了甘薯引进种的遗传多样性, 拓宽了原有资源库中的群体结构。本文通过SSR引物对378份甘薯引进种种质进行遗传多样性和群体结构分析, 可为甘薯种质创新、遗传育种、优异基因挖掘利用提供参考。
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