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作物学报 ›› 2025, Vol. 51 ›› Issue (1): 247-259.doi: 10.3724/SP.J.1006.2025.44044

• 研究简报 • 上一篇    下一篇

马铃薯转录因子StFBH3对非生物逆境胁迫的响应分析

宋倩娜1,2(), 宋慧洋1, 李京昊1, 段永红1, 梅超1,2, 冯瑞云1,2,*()   

  1. 1山西农业大学农学院, 山西太谷 030801
    2山西省农业科学院作物科学研究所, 山西太原 030031
  • 收稿日期:2024-03-07 接受日期:2024-09-18 出版日期:2025-01-12 网络出版日期:2024-09-23
  • 通讯作者: *冯瑞云, E-mail: fengruiyun1970@163.com
  • 作者简介:E-mail: songqianna1007@126.com
  • 基金资助:
    山西省农业科学院作物科学研究所博士基金项目(ZB1102);山西农业大学博士基金项目(2021BQ44);山西省博士毕业生来晋工作奖励资金科研项目(SXBYKY2021084);山西省基础研究计划项目(202203021211272);山西农业大学农学院育种工程专项(YZ2021-04)

Response of transcription factor StFBH3 under abiotic stress in potato

SONG Qian-Na1,2(), SONG Hui-Yang1, LI Jing-Hao1, DUAN Yong-Hong1, MEI Chao1,2, FENG Rui-Yun1,2,*()   

  1. 1College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China
  • Received:2024-03-07 Accepted:2024-09-18 Published:2025-01-12 Published online:2024-09-23
  • Contact: *E-mail: fengruiyun1970@163.com
  • Supported by:
    Doctoral Fund of Institute of Crop Science, Shanxi Academy of Agricultural Sciences(ZB1102);Doctoral Fund of Shanxi Agricultural University(2021BQ44);Doctoral Award Fund Research Project of Shanxi Province(SXBYKY2021084);Basic Research Foundation of Shanxi Province(202203021211272);Breeding Engineering Special Project of College of Agriculture, Shanxi Agricultural University(YZ2021-04)

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摘要：

bHLH (basic helix-loop-helix)作为植物界第二大类转录因子, 在植物应对环境胁迫响应中起着重要的调控作用。探究马铃薯(Solanum tuberosum L.) bHLH家族基因功能将为马铃薯改良和育种提供一定的理论依据。本研究克隆了马铃薯StFBH3基因(Gene ID: 102582309), 利用qPCR技术分析了不同逆境胁迫下StFBH3基因表达模式, 结果表明, StFBH3基因在马铃薯根和叶中的表达量较高, 且该基因的表达受渗透、高盐和脱落酸(abscisic acid, ABA)诱导; 以过表达StFBH3基因的马铃薯试管苗为材料, 在分别含有不同浓度甘露醇(mannitol)、NaCl和ABA的MS培养基中, 过表达StFBH3马铃薯株系的叶绿素含量显著高于野生型, 根长显著长于野生型。在干旱和高盐处理下, 土壤栽培的过表达马铃薯株系较野生型表现较强的耐受性, 叶片相对含水量、叶绿素含量和过氧化物歧化酶(SOD)活性显著高于野生型。qPCR分析发现, 干旱和盐胁迫处理下相关基因(KAT1)的表达量在过表达马铃薯株系中较野生型显著降低。以上结果表明, StFBH3基因可能在马铃薯对渗透、干旱和高盐等胁迫响应中起正向调控作用。本研究也为StFBH3基因在马铃薯中的生物学功能深入理解提供一定的参考依据。

关键词: 马铃薯, StFBH3, 转录因子, 逆境胁迫

Abstract:

The basic helix-loop-helix (bHLH) transcription factor family is the second largest family of transcription factors and plays a central regulatory role in enabling plants to defend against environmental stress. Investigating the function of bHLH family genes in potato can provide a theoretical foundation for potato improvement and breeding. In this study, the StFBH3 gene was cloned, and its tissue-specific expression patterns under different stress treatments were analyzed using qPCR. The relative expression level of StFBH3 was highest in potato roots and leaves, and its expression was induced by osmotic stress, high salinity, and abscisic acid (ABA) treatment. On MS medium containing various concentrations of mannitol, NaCl, and ABA, the chlorophyll content in the StFBH3 overexpressing potato lines was significantly higher than that of the wild type, and the root length was notably longer. Under drought and high salinity conditions, the StFBH3 overexpressing lines cultivated in soil exhibited stronger tolerance compared to the wild type, with significantly higher leaf relative water content, chlorophyll content, and superoxide dismutase (SOD) enzyme activity. Furthermore, qPCR analysis revealed that the expression levels of drought- and salt stress-related genes, such as KAT1, were significantly reduced in the StFBH3overexpressing lines compared to the wild type. These results suggest that StFBH3 functions as a positive regulator, induced and specifically expressed in response to osmotic stress, drought, and high salinity. This study provides an important reference for further analysis of the biological function of the StFBH3 gene in potato.

Key words: potato, StFBH3, transcription factor, adversity stress

表1

本研究所用引物"

引物名称
Prime name		 引物序列
Primer sequence (5'-3')
StFBH3-F ATGGATTCATCAGAGTTTGAG
StFBH3-R TTTGCAGTTAGCGCGACAGTCA
HYG-F GATCGGACGATTGCGTCGCAT
HYG-R CGTGGATATGTCCTGCGGGTAA
qStFBH3-F GTGGGTACAGGGTGATGGGG
qStFBH3-R CTCCAGCACCTTTCAAC
qStKAT1-F GGATCTGCTTTTCCAACTGGTTC
qStKAT1-R CGAGCAACAGCCTTCCCAAT
Actin-F ATTGGAAACGGATATGCTCCA
Actin-R TCCTTACCTGAACGCCTGTCA

图1

StFBH3的序列分析 A: 氨基酸多序列比对。黑线表示C1、C2和C3保守结构域; 黑色框表示保守的磷酸化位点。B: 进化树分析。0.1标尺表示每单位核苷酸变化的数量。C: 蛋白空间结构预测。D: 保守结构域分析。"

图2

StFBH3在马铃薯不同组织和非生物胁迫处理下的相对表达量 A: StFBH3在不同组织中的相对表达量; B~D: StFBH3在高盐、脱水和ABA处理后的相对表达量。*表示在0.05概率水平差异显著。"

图3

不同浓度甘露醇和盐处理下过表达马铃薯株系中叶绿素含量比较 A和C: 不同浓度甘露醇和盐处理下, 过表达马铃薯株系与野生型植株叶片的表型; B和D: 不同浓度甘露醇和盐处理下, 过表达马铃薯株系与野生型植株叶片中叶绿素含量。**表示在0.01概率水平差异显著。"

图4

在NaCl和甘露醇处理下过表达马铃薯株系的生根率、根长和根数比较 A~B: 100 mmol L-1NaCl和350 mmol L-1 甘露醇处理下的马铃薯株系形态学; C~E: 100 mmol L-1 NaCl和350 mmol L-1 甘露醇处理下马铃薯株系的生根率、主根长度和主根数。标尺为1.0 cm; **表示在0.01概率水平差异显著。"

图5

ABA和甘露醇处理下过表达马铃薯株系的生根率、根长和根数比较 A: 100 µmol L-1 ABA处理下的马铃薯株系形态学; B: 50 µmol L-1 ABA和350 mmol L-1 甘露醇处理下的马铃薯株系形态学; C~E: 2种条件处理下马铃薯株系的生根率、主根长度和主根数量。标尺为1.0 cm; **表示在0.01概率水平差异显著。"

图6

土壤中StFBH3过表达马铃薯株系在干旱胁迫下的表型分析 A: 马铃薯植株在干旱胁迫下的表型; B: 干旱胁迫下, 马铃薯株系地上部位的长度; C: 干旱胁迫下, 马铃薯株系叶片的相对含水量; D: 干旱胁迫下, 马铃薯株系叶片中SOD酶活性分析; E: 干旱胁迫下, 马铃薯株系的叶绿素含量测定。标尺为1.0 cm; **表示在0.01概率水平差异显著。"

图7

土壤中StFBH3过表达马铃薯株系在盐胁迫下的表型分析 A: 马铃薯植株在盐胁迫下的表型; B: 在盐胁迫下, 马铃薯株系地上部位的长度; C: 在盐胁迫下, 马铃薯株系叶片的相对含水量; D: 在盐胁迫下, 马铃薯株系叶片中SOD酶活性分析; E: 在盐胁迫下, 马铃薯株系的叶绿素含量测定。标尺为1.0 cm; **表示在0.01概率水平差异显著。"

图8

干旱和盐胁迫响应基因在马铃薯中的表达分析 **表示在0.01概率水平差异显著。"

表2

50 mmol L-1 NaCl处理下过表达马铃薯株系单株试管薯鲜重"

样本
Groups		 每株平均试管薯鲜重Micro-tuber per plant
0 mmol L-1 NaCl 50 mmol L-1 NaCl
CK 34.68 ± 14.86 a 11.50 ± 7.80 a
OE-1 48.65 ± 27.35 a 21.23 ± 6.92 a
OE-2 35.00 ± 12.02 a 13.16 ± 1.46 a
OE-3 38.44 ± 9.75 a 18.35 ± 16.73 a
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