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作物学报 ›› 2025, Vol. 51 ›› Issue (2): 347-357.doi: 10.3724/SP.J.1006.2025.42030

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻矮化多分蘖基因DT1的图位克隆与功能分析

李春梅，陈洁，郎兴宣，庄海民，朱靖，杜梓君，冯浩天，金涵，朱国林，刘凯*   

  1. 仲恺农业工程学院, 广东广州 510225
  • 收稿日期:2024-06-25 修回日期:2024-09-18 接受日期:2024-09-18 出版日期:2025-02-12 网络出版日期:2024-10-10
  • 基金资助:
    本研究由西南作物基因资源发掘与利用国家重点实验室开放基金课题(SKL-KF202315), 省部共建农产品质量安全危害因子与风险防控国家重点实验室开放基金课题(2021DG700024-KF202408), 广东省基础与应用基础研究基金项目(2024A1515013028), 云浮市市科技计划项目(2023020202), 广东省普通高校特色创新类项目(2023KTSCX046)和广州市科技计划项目(2024A04J4995)资助。

Map-based cloning and functional analysis of Dwarf and Tillering 1 (DT1) gene in rice

LI Chun-Mei,CHEN Jie,LANG Xing-Xuan,ZHUANG Hai-Min,ZHU Jing,DU Zi-Jun,FENG Hao-Tian,JIN Han,ZHU Guo-Lin,LIU Kai*   

  1. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
  • Received:2024-06-25 Revised:2024-09-18 Accepted:2024-09-18 Published:2025-02-12 Published online:2024-10-10
  • Supported by:
    This study was supported by the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China (SKL-KF202315), the State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products (2021DG700024-KF202408), the Basic and Applied Basic Research Foundation of Guangdong Province (2024A1515013028), the Science and Technology Plan Project of Yunfu (2023020202), the Guangdong Provincial Universities Characteristic Innovation Project (2023KTSCX046), and the Guangzhou Science and Technology Plan Project (2024A04J4995).

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摘要：

分蘖是影响水稻株型和产量的重要性状。本研究获得一个稳定遗传的矮化多分蘖自然突变体dt1 (dwarf and tillering 1)。此外，dt1突变体穗长、结实率、粒长、粒宽、千粒重、维管束鞘细胞数量及大小较野生型均显著降低。图位克隆证实dt1突变体是由编码独脚金内酯生物合成途径关键酶类胡萝卜素裂解双加氧酶(Carotenoid Cleavage Dioxygenase 7，CCD7)的D17/HTD1 (LOC_Os04g46470)第2个外显子上8 bp的插入导致的，是一个D17/HTD1的新等位突变。此外，dt1突变体萌发率、根长、根直径均显著降低，外施独脚金内酯类似物GR24能恢复dt1突变体的这些表型。转录组测序结果显示，dt1突变体有579个基因上调，506个基因下调。GO分析显示上调基因显著富集在生长素响应、内源刺激响应和激素响应等通路，下调基因显著富集在胞内碳水化合物代谢和组蛋白甲基化等通路。KEGG分析显示上调基因在植物激素信号转导等通路显著富集，下调基因在氨基糖和核苷酸糖代谢及二萜生物合成等通路上显著富集。研究结果丰富和拓展了CCD7和独脚金内酯在水稻中的调控作用，对水稻育种具有重要理论意义。

关键词: 水稻, 矮化多分蘖, 图位克隆, 生物学功能

Abstract:

Tillering is a crucial trait that influences plant architecture and yield in rice. In this study, we identified a natural mutant with dwarf stature and high tillering, which we designated as dwarf and tillering 1 (dt1). The dt1 mutant exhibited significant reductions in panicle length, seed setting rate, grain length, grain width, thousand-grain weight, and the number and size of vascular bundle sheath cells compared to the wild type. Map-based cloning revealed that the dt1 phenotype was caused by an 8 bp insertion in the second exon of D17/HTD1 (LOC_Os04g46470), which encodes Carotenoid Cleavage Dioxygenase 7 (CCD7), a key enzyme in strigolactone biosynthesis. Thus, dt1 represents a new allele of D17/HTD1. Additionally, the dt1 mutant showed significantly reduced germination rate, root length, and root diameter, all of which were restored by the exogenous application of the strigolactone analog GR24. Transcriptomic analysis identified 579 up-regulated and 506 down-regulated genes in the dt1 mutant. Gene Ontology (GO) analysis revealed that the up-regulated genes were significantly enriched in pathways related to auxin response, endogenous stimulus response, and hormone response, while the down-regulated genes were enriched in pathways involved in cellular carbohydrate metabolism and histone methylation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the up-regulated genes were associated with plant hormone signal transduction, whereas the down-regulated genes were linked to amino sugar and nucleotide sugar metabolism, as well as diterpenoid biosynthesis. These findings enhance our understanding of the regulatory roles of CCD7 and strigolactones in rice and hold significant theoretical implications for rice breeding.

Key words: rice, dwarf and tillering, map-based cloning, biological function

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