欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (6): 992-997.doi: 10.3724/SP.J.1006.2009.00992

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯晚疫病抗性基因R11的遗传定位

徐建飞，金黎平，段绍光，黄三文，屈冬玉*   

  1. 中国农业科学院蔬菜花卉研究所，北京100081
  • 收稿日期:2008-11-24 修回日期:2009-02-17 出版日期:2009-06-12 网络出版日期:2009-04-16
  • 通讯作者: 屈冬玉，E-mail: dyqu@mail.caas.net.cn;Tel:010-82109543
  • 作者简介:徐建飞，E-mail:xujianfei1026@126.com
  • 基金资助:

    本研究由国家自然科学基金项目（30671319），国家科技支撑计划（2007BAD49A01），农业部园艺作物遗传改良重点开放实验室资助。

Genetic Mapping of Rll Gene Conferring Resistance to Late Blight in Potato(Solanum tuberosum)

XU Jian-Fei,JIN Li-Ping,DUAN Shao-Guang,HUANG San-Wen,QU Dong-Yu*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-11-24 Revised:2009-02-17 Published:2009-06-12 Published online:2009-04-16
  • Contact: QU Dong-Yu，E-mail: dyqu@mail.caas.net.cn;Tel:010-82109543
  • About author:XU Jian-Fei，E-mail:xujianfei1026@126.com

PDF

2102

被引次数

11

摘要：

由致病疫霉菌(Phytophthora infestans)引起的晩疫病是世界范围内最具毁灭性的马铃薯(Solanum tuberosum)病害。以含有晚疫病抗性基因R11的材料MaR11和不含已知抗性基因的品种Katahdin为亲本进行有性杂交，对获得的F1分离群体的83个基因型进行了晚疫病菌株接种鉴定和遗传分析。结果表明，R11为主效单基因，在MaR11中以单式形式(R11r11r11r11)存在。应用比较作图和分离群体分组分析(BSA)，开发了6个与R11连锁的分子标记，并将R11定位于11号染色体长臂末端。R11距C2_At5g59960标记最近，约为2.4 cM。通过遗传图谱比较表明，R11较晚疫病抗性基因R3a和R10更靠近染色体端粒区。本研究所获得的遗传图谱为进一步构建R11高密度遗传图谱提供了基础。

关键词: 马铃薯, 晚疫病, Rll , 遗传定位

Abstract:

Late blight caused by Phytophthora infestans is the most destructive disease for potato (Solanum tuberosum). A F1 segregating population consisting of 83 genotypes, derived from R11 differential MaR11 and the susceptible potato cultivar Katahdin, was assessed for late blight resistance in detached leaves assay and genetically analyzed. The results showed that the distribution for R11 was clearly bimodal with the two peaks coinciding with the blight scores of the MaR11 (the resistant parent) and Katahdin (the susceptible parent), and the observed segregation with 39 resistant and 44 susceptible plants fitted a 1:1 ratio. It indicated that R11 was inherited as a major dominant R gene and presented in the simplex condition in MaR11. There was a major late blight resistance locus (MLB) in potato on the long arm of chromosome 11, where R11 showed allelic versions of the R3 and R10 loci. Using the combination of comparative mapping and bulked segregant analysis, we developed six markers linked to R11 which was located on the end of the long arm of chromosome 11 with a distance of about 2.4 cM to marker C2_At5g59960. R11 was telomeric to the R3a and R10 gene. The genetic map constructed in this paper provides a basis for further construction of high-resolution genetic map of the R11 gene.

Key words: Potato, Late blight, Rll, Genetic mapping

[1] McDonald B A, Linde C. Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol, 2002, 40: 349-379

[2] Andrivon D, Lucas J M, Ellissèche D. Development of natural late blight epidemics in pure and mixed plots of potato cultivars with different levels of partial resistance. Plant Pathol, 2003, 52: 586-594
[3] Dangl J L, Jones J D. Plant pathogens and integrated defense responses to infection. Nature, 2001, 411: 826-833

[4] Mundt C C. Use of multiline cultivars and cultivar mixtures for disease management. Annu Rev Phytopathol, 2002, 40: 381-410

[5] Ballvora A, Ercolano M R, Weiss J, Meksem K, Bormann C A, Oberhagemann P, Salamini F, Gebhardt C. The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant J, 2002, 30: 361-371

[6] Huang S, van der Vossen E A, Kuang H, Vleeshouwers V G, Zhang N, Borm T J, van Eck H J, Baker B, Jacobsen E, Visser R G. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant J, 2005, 42: 251-261

[7]Song J, Bradeen J M, Naess S K, Raasch J A, Wielgus S M, Haberlach G T, Liu J, Kuang H, Austin-Phillips S, Buell C R, Helgeson J P, Jiang J. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci USA, 2003, 100: 9128-9133

[8] Huang S. Discovery and characterization of the major late blight resistance complex in potato: Genomic structure, functional diversity, and implications. Wageningen Netherlands: Wageningen Universiteit (Wageningen University), 2005

[9] Bradshaw J E, Bryan G J, Lees A K, McLean K, Solomon-Blackburn R M. Mapping the R10 and R11 genes for resistance to late blight (Phytophthora infestans) present in the potato (Solanum tuberosum) R-gene differentials of black. Theor Appl Genet, 2006, 112: 744-751

[10] Vleeshouwers V G, van Dooijeweert W, Keizer L C P, Sijpkes L, Govers F, Colon L T. A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation. Eur J Plant Pathol, 1999, 105: 241-250

[11] van der Lee T, Testa A, van’t Klooster J, van den Berg-Velthuis G, Govers F. Chromosomal deletion in isolates of Phytophthora infestans correlates with virulence on R3, R10, and R11 potato lines. Mol Plant Microbe Interact, 2001, 14: 1444-1452
[12] Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman j, Kuiper M, Zabeau M. AFLP: A new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[13] Malcolmson J F, Black W. New R-genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica, 1966, 15: 199-203
[14] Flor H H. Current status of the gene-for-gene concept. Annu Rev Phytopathol, 1971, 9: 276-296
[15] Xu J-F(徐建飞), Wang J-J(王加加), Li Y(李颖), Jin L-P(金黎平), Jacobsen E, Huang S-W(黄三文), Qu D-Y(屈冬玉). Genetic mapping of the R10 gene for resisting late blight in potato by comparative genomics. Sci Technol Rev (科技导报), 2007, 25: 30-35(in Chinese with English abstract)
[16] Harrison J G, Lowe R. Stability of R-gene resistance to late blight in potato leaves. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. 365-367
[17] Turkensteen L J. Interaction of R-genes in breeding for resistance of potatoes against Phytophthora infestans. In: Rep of the Planning Conf: Fungal Diseases of the Potato. Lima: International Potato Center, 1989. pp 85-96
[18] Stewart H E, Gourlay F. Recognising race-specific resistance to Phytophthora infestans. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. pp 255-260
[19] Brouwer D J, Jones E S, St. Clair D A. QTL analysis of quantitative resistance to Phytophthora infestans (late blight) in tomato and comparisons with potato. Genome, 2004, 47: 475-492
[20] Li X, van Eck H J, Rouppe van der Voort J N A M, Huigen, D J, Stam P, Jacobsen E. Autotetraploids and genetic mapping using common AFLP markers: The R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet, 1998, 96: 1121-1128
[21] Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C. The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet,1992, 233: 278-283
[22] Huang S, Vleeshouwers V G A A, Werij J S, Hutten R C B, van Eck H J, Visser R G F, Jacobsen E. The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant Microbe Interact,2004, 17: 428-435
[23] Naess S K, Bradeen J M, Wielgus S M, Haberlach G T, McGrath J M, Helgeson J P. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet,2000, 101: 697-704
[24] Colon L T, Budding D J. Hoogendoorn J. Breeding for foliar resistance to Phytophthora infestans in potato: the influence of test conditions on the results of screening for field resistance. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. pp 282-288
[25] Meyers B C, Chin D B, Shen K A, Sivaramakrishnan S, Lavelle D O, Zhang Z, Michelmore R W. The major resistance gene cluster in lettuce is highly duplicated and spans several megabases. Plant Cell, 1998, 10: 1817-1832
[1] 陈国欢, 张锐, 李艳迪, 赵佳琪, 任湧涛, 张天赐, 郭华春, 李俊, 杨芳. 外源硒叶面喷施对浅紫色马铃薯块茎花青素合成的影响[J]. 作物学报, 2026, 52(6): 1876-1890.
[2] 王文辕, 燕雪嘉, 刘玉霖, 孙晓彤, 李亚楠, 唐鑫华, 石瑛. 耐弱光马铃薯品种筛选及转录因子编码基因StPIF3的克隆与功能分析[J]. 作物学报, 2026, 52(6): 1631-1645.
[3] 田立涛, 丁宁, 王树林, 齐恩芳, 张荣, 王蕊蕊, 马利雯, 李建武, 杨江伟. 马铃薯Argonaute基因家族鉴定及晚疫病诱导表达分析[J]. 作物学报, 2026, 52(4): 1116-1126.
[4] 汪玲, 胡好, 宋家凤, 程洁蓝, 陈颖, 郑婷婷, 吕钊彦, 朱晓彪, 侯华兰. 马铃薯UDP-糖基转移酶基因StUGT52的克隆及功能验证[J]. 作物学报, 2026, 52(3): 665-676.
[5] 张宇, 刘芳, 蔡诚诚, 杨小华, 吉阿么石扎, 杨元军, 王西瑶. 溴乙烷与赤霉素协同处理破除马铃薯块茎休眠的机理初探[J]. 作物学报, 2026, 52(3): 825-838.
[6] 杨飚, 杜帅康, 张继旺, 石瑛, 张丽莉. 马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析[J]. 作物学报, 2026, 52(2): 405-420.
[7] 徐强, 谢奎忠, 胡新元, 岳云, 董博, 罗爱花. 连作对马铃薯根际土壤线虫群落结构与功能的影响[J]. 作物学报, 2026, 52(2): 527-538.
[8] 姬炫彤, 卞春松, 金黎平, 李森, 秦军红, 李广存. 不同耐旱型马铃薯根际微生物对干旱的响应[J]. 作物学报, 2026, 52(1): 165-177.
[9] 田甲春, 葛霞, 李守强, 李梅, 田世龙, 张亚倩, 程建新, 李玉梅. 低O2高CO2贮藏环境延缓马铃薯块茎衰老的作用机制[J]. 作物学报, 2026, 52(1): 262-278.
[10] 王雅致, 杨飚, 季香林, 石瑛, 张丽莉. 二倍体马铃薯抗旱资源鉴定及抗旱基因初步筛选[J]. 作物学报, 2026, 52(1): 72-84.
[11] 卓峰琦, 唐振三, 雷雨俊, 程李香, 赵甜甜, 吕汰, 杨晨, 张峰. 基于烹饪方式及回生温度筛选低升糖马铃薯品种(系)[J]. 作物学报, 2025, 51(9): 2538-2546.
[12] 朱锦程, 杨秋华, 程李香, 李文丽, 石明明, 李惠霞, 张峰. 马铃薯抗南方根结线虫种质资源筛选及相关生理反应分析[J]. 作物学报, 2025, 51(9): 2307-2317.
[13] 尹丽娜, 张锐, 陈国欢, 白磊, 李俊, 郭华春, 杨芳. 不同马铃薯品种块茎创伤愈合能力的比较[J]. 作物学报, 2025, 51(9): 2399-2411.
[14] 贾小霞, 齐恩芳, 文国宏, 马胜, 黄伟, 吕和平, 李建武, 曲亚英, 丁宁. 中早熟马铃薯‘陇薯20号’高效再生体系建立及抗草铵膦种质创制[J]. 作物学报, 2025, 51(9): 2285-2294.
[15] 邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2