作物学报 ›› 2013, Vol. 39 ›› Issue (09): 1602-1611.doi: 10.3724/SP.J.1006.2013.01602
靳义荣1,2,白岩1,2,宋毓峰1,2,张良1,2,董连红1,2,刘朝科3,冯祥国3,胡晓明3,王倩,*,刘好宝1,*
JIN Yi-Rong1,2,BAI Yan1,2,SONG Yu-Feng1,2,ZHANG Liang1,2,DONG Lian-Hong1,2,LIU Chao-Ke3,FENG Xiang-Guo3,HU Xiao-Ming3,WANG Qian1,*,LIU Hao-Bao1,*
摘要:
Shaker家族钾离子通道在植物钾的吸收转运及其他生命过程中发挥重要作用。本研究利用同源克隆的策略从林烟草中获得一个Shaker家族钾离子通道基因,命名为NKT6 (GenBank登录号为KC310448)。该基因cDNA序列全长2 317 bp,编码由681个氨基酸组成的蛋白,该蛋白与Shaker家族其他成员具有较高同源性。NKT6基因组CDS序列共含有11个外显子、10个内含子。系统进化树分析表明,NKT6蛋白是Shaker家族Group II的成员之一。荧光定量PCR分析发现,NKT6的表达量在林烟草的茎和腋芽中最高,在萼片、叶、花中其次,在根中最低。亚细胞定位结果表明,NKT6主要定位于细胞膜和核膜附近的内质网上。干旱与外源ABA胁迫处理下,NKT6的表达量均呈下降趋势。推测NKT6可能在林烟草气孔开放中发挥作用。
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