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作物学报 ›› 2017, Vol. 43 ›› Issue (10): 1499-1506.doi: 10.3724/SP.J.1006.2017.01499

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

新型香稻渝恢2103香味分子遗传特性分析

王春萍1,**,张现伟2,**,白文钦1,蒋晓英1,吴红1,林清1,唐永群2,姚雄2,张巫军2,唐荣莉1,李经勇2,*,雷开荣1,*   

  1. 1 重庆市农业科学院生物技术研究中心, 重庆 401329; 2重庆市农业科学院重庆再生稻研究中心, 重庆 402160
  • 收稿日期:2016-12-05 修回日期:2017-04-19 出版日期:2017-10-12 网络出版日期:2017-05-23
  • 通讯作者: 李经勇, E-mail: ljy@cqagri.gov.cn; 雷开荣, E-mail: leikairong@126.com
  • 基金资助:

    本研究由重庆市农发良种创新暨重大科研推广项目(NKY2016AA003), 重庆市农发基础科研项目(NKY2016AC024), 重庆市基础与前沿研究计划项目(cstc2016jcyjA0091)和重庆市基本科研业务费(2014cstc-jbky-00549)资助。

Molecular Genetic Characters of Fragrance in a New Fragrant Rice Variety Yuhui 2103

WANG Chun-Ping1,**,ZHANG Xian-Wei2,**,BAI Wen-Qin1,JIANG Xiao-Ying1,WU Hong1,LIN Qing1,TANG Yong-Qun2,YAO Xiong2,ZHANG Wu-Jun2,TANG Rong-Li,LI Jing-Yong2,*,LEI Kai-Rong1,*   

  1. 1 Biotechnology Research Center, Chongqing  Academy of Agricultural Sciences, Chongqing 401329, China; 2 Chongqing Ratooning Rice Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China
  • Received:2016-12-05 Revised:2017-04-19 Published:2017-10-12 Published online:2017-05-23
  • Contact: 李经勇, E-mail: ljy@cqagri.gov.cn; 雷开荣, E-mail: leikairong@126.com
  • Supported by:

    This work was supported by Chongqing Agriculture Seed Innovation and Major Research Extension Project (NKY2016AA003), Chongqing Agriculture Basic Research Project (NKY2016AC024), Chongqing Basic and Frontier Research Program (cstc2016jcyjA0091) and Chongqing Basic Research Fund (2014cstc-jbky-00549).

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摘要：

香味是优良稻米品质的重要衡量标准之一, 2-乙酰-1-吡咯啉(2AP)是最主要的香味物质, 然而2AP生物合成机理至今仍未确凿。本研究筛选了与2AP生物合成密切相关的甜菜碱脱氢酶2基因(Badh2)在30份水稻材料中的3种突变类型, 从中发现1份新的香稻材料渝恢2103, 该材料Badh2基因序列编码区无突变, 遗传分析显示渝恢2103与badh2-E7突变型香稻宜香1B香味基因不等位, 与非香稻杂交F2香与非香分离比接近9∶7, 与香稻杂交F2香与非香分离比接近7∶9, 表明渝恢2103的香味受多基因控制。进一步利用实时荧光定量PCR技术(qRT-PCR)比较了与2AP生物合成相关基因在日本晴、渝恢2103和宜香1B的表达情况, 结果显示, Badh2基因在日本晴和渝恢2103中表达差异不大, 但在宜香1B中表达量异常高; 多数脯氨酸与谷氨酸代谢途径相关基因在宜香1B中的表达水平显著高于日本晴和渝恢2103; 推测宜香1B的2AP合成同时受Badh2基因以及脯氨酸与谷氨酸代谢途径相关基因的影响; 渝恢2103香味形成可能与这些基因无必然联系。渝恢2103特殊的遗传特性可能为水稻香味形成机理研究提供新的突破点。

关键词: 香稻, 2-乙酰-1-吡咯啉, 脯氨酸, 谷氨酸, Badh2, 遗传特性

Abstract:

A major component 2-Acetyl-1-pyrroline (2AP) in fragrance is one of the important indices of high-quality rice, however, the biosynthetic pathway of 2AP has not been demonstrated clearly. The betaine aldehyde dehydrogenase 2 (BADH2) is considered to be closely related to 2AP biosynthesis. In this study, three mutations in Badh2 gene were screened from thirty rice varieties and a new fragrant rice variety Yuhui 2103 was discovered. The new variety has no mutation in the coding region of Badh2 gene and the Badh2 allele of Yuhui 2103 can complement the defect of the badh2-E7 allele in Yixiang 1B. From crosses between Yuhui 2103 and non-fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 9:7, while from crosses between Yuhui 2103 and fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 7:9, indicating that fragrance of Yuhui 2103 is not controlled by only one gene. Furthermore, expression patterns of genes involved in 2AP biosynthesis were examined by the quantitative real-time PCR (qRT-PCR) in Nipponbare, Yuhui 2103, and Yixiang 1B. There was no significant difference in transcription level of Badh2 in Nipponbare and Yuhui 2103, however, the transcription level of Badh2 inYixiang 1B was unexpectedly high. The expression levels of most of the genes involved in proline and glutamatic acid metabolism were significantly higher in Yixiang 1B than that in Nipponbare and Yuhui 2103. It is proposed that 2AP biosynthesis in Yixiang 1B is both influenced by Badh2 and the genes involved in proline and glutamatic acid metabolism, however, there is no necessary relationship between these genes and the fragrance of Yuhui 2103. The novel genetic characters of Yuhui 2103 may bring new breakthrough to the study of fragrance formation mechanism in rice.

Key words: Fragrant rice, 2-Acetyl-1-pyrroline, Proline, Glutamatic acid, Badh2, Genetic character

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