作物学报 ›› 2022, Vol. 48 ›› Issue (9): 2390-2399.doi: 10.3724/SP.J.1006.2022.11070
李永波1(), 崔德周1, 黄琛1, 隋新霞1, 樊庆琦1,*(
), 楚秀生1,2,*
LI Yong-Bo1(), CUI De-Zhou1, HUANG Chen1, SUI Xin-Xia1, FAN Qing-Qi1,*(
), CHU Xiu-Sheng1,2,*
摘要:
细胞自噬是一种进化上高度保守的溶酶体/液泡降解途径, 对机体适应各种生物或非生物胁迫, 以及维持自身正常生长发育具有重要的意义。自噬相关蛋白8 (autophagy-related protein 8, ATG8)是检测自噬的金标准。目前由于缺乏特异性好的小麦ATG8抗体, 导致小麦细胞自噬研究进展缓慢。本研究通过人工合成小麦ATG8蛋白序列上的一段18个氨基酸的多肽作为抗原去免疫兔子, 成功获得了高度特异性的小麦ATG8多克隆抗体。该抗体不仅能够识别小麦根、叶及种子中的ATG8蛋白条带, 而且可以在根、叶中检测到细胞自噬结构, 并首次在小麦籽粒中实现了细胞自噬结构的检测。本研究研制的小麦ATG8抗体, 除了能够检测到小麦中常见的ATG8a-h等8种典型类型外, 还能检测到一种非典型的新的ATG8蛋白, 为深入开展植物细胞自噬调控机制研究以及优异新基因挖掘提供了最佳方法学基础。
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