Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1851-1857.doi: 10.3724/SP.J.1006.2009.01851


A New Rice Cultivar“Wulingjing1” with Resistance to Rice Stripe Virus Bred by Marker Assisted Selection

PAN Xue-Biao1,*,CHEN Zong-Xiang1,ZUO Shi-Min1,ZHANG Ya-Fang1,WU Xu-Jiang1,MA Ning1,JIANG  Qi-Xiang2,QUE Jin-Hua3,ZHOU  Chun-He3   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Education Ministry, Yangzhou University, Yangzhou 225009, China; 2 Wujin Rice and Wheat Breeding Station, Changzhou 213149, China; 3 Seeds Management Station of Jiangsu Province, Nanjing 210036, China
  • Received:2009-02-02 Revised:2009-04-27 Online:2009-10-12 Published:2009-08-07
  • Contact: PAN Xue-Biao, E-mail: shuidao@yzu.edu.cn


Rice stripe virus disease is one of the most serious diseases in rice production area of Jiangsu province since 2002. A commercial rice cultivar with excellent food taste-quality, Wuyujing 3, was highly susceptible to the disease and was improved through the MAS strategy in a backcross project with “Zhendao 88” as donor parent of resistance gene Stvb-i. In each backcross generation, we selected the progenies with Stvb-i based on the closely flanked markers. In advanced backcross generations, the marker’s detection for genetic background of selected plants was performed, along with the estimate by the eye for the similarity of selected plants to recurrent parent in synthetic agronomic traits. As a result, a new cultivar, Wulingjing 1, was fleetingly bred in BC3F4 generation, whose resistance to the disease was significantly increased and main characters especially on excellent taste-quality were consistent with those of the recurrent parent Wuyujing 3. In resistance identification tests with multiple locations organized by Seed Management Station of Jiangsu Province, the average percent of affected plants of the new cultivar was only 4.4% and significantly lower than that of recurrent parent (53.2%).

Key words: Rice stripe disease, Resistance gene donor, Backcross breeding, Current parent, MAS

[1] Chen Z-D(陈志德), Zhong W-G(仲维功), Yang J(杨杰), Wang J(王军). Study on the yielding traits and quality characters of Wu yujing 3 and the suggestion for rice breeding. J金陵科技学院学报), 2007, 23(4): 50-53 (in Chinese with English abstract)(

[2] Jiang Q-X(江祺祥), Yang M-F(杨明方), Wang Z-M(王子明), Qian Y-X(钱永祥). A new japonica rice Wuyujing 3 with middle length of the whole growth stage, its breeding and commercial spread. J Jiangsu Agric Sci (江苏农业科学), 1993, (3): 8-10 (in Chinese)

[3] Lu B-G(卢白关), Xu D-Y(徐大勇), Fang Z-W(方兆伟), Qin D-R(秦德荣), Liu H-Q(刘汉青), Pan J-W(攀技伟). Investigation of rice stripe disease developed on some japonica rice cultivars. J Jiangsu Agric Sci (江苏农业科学), 2006, (4): 43-45 (in Chinese)

[4] Chen T(陈涛), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Lin J(林静), Zhang S-B(张所兵), Wang C-L(王才林). Research progress of resistant genetics and breeding against rice stripe disease. J Jiangsu Agric Sci (江苏农业科学), 2006, (2): 1-4 (in Chinese)

[5] Hayano Y, Saito K, Nakamura S, Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stvb-i. Theor Appl Genet, 2000, 101: 59-63

[6] Hayano Y, Tsuji T, Fujii K, Saito K, Iwasaki M, Saito A. Loca-lization of the rice stripe disease resistance gene Stvb-i by graphical genotyping and linkage analyses with molecular markers. Theor Appl Genet, 1998, 96: 1044-1049

[7] Sun D-Z(孙黛珍), Jiang L(江玲), Zhang Y-X(张迎信), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection of QTLassociated with rice stripe resistance in cultivar IR24. Acta Agron Sin (作物学报), 2007, 33(1): 25-30 (in Chinese with English abstract)

[8] Ding X-L(丁秀兰), Jiang L(江玲), Liu S-J(刘世家), Wang C-M(王春明), Chen L-M(陈亮明), Cheng Z-B(程兆榜), Fan Y-J(范永坚), Zhou Y-J(周益军), Wan J-M(万建民). QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of Kinmaze and DV85. Acta Genet Sin (遗传学报), 2004, 31(3): 287-292 (in Chinese with English abstract)

[9] Wu S J, Zhong H, Zhou Y, Zuo H, Zhou L H, Zhu J Y, Ji C Q, Gu S L, Gu M H, Liang G H. Identification of QTLs for the resistance to rice stripe virus in the indica rice variety Dular. Euphytica, 2009, 165: 557-567

[10] Pan X-B(潘学彪), Liang G-H(梁国华), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳). Breeding strategy on resistance to rice stripe in Jiangsu. J Jiangsu Agric Sci (江苏农业科学), 2005, (5): 22-23 (in Chinese) Pan C-H(潘存红), Wang Z-B(王子斌), Ma Y-Y(马玉银), Yin Y-J(殷跃军), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Chen Z-X(陈忠祥), Pan X-B(潘学彪). InDel and SNP markers and their application in map-based cloning of rice genes. Chin J Rice Sci (中国水稻科学), 2007, 21(5): 447-453 (in Chinese with English abstract)

[1] WANG Wang-Nian, GE Jun-Zhu, YANG Hai-Chang, YIN Fa-Ting, HUANG Tai-Li, KUAI Jie, WANG Jing, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong. Adaptation of feed crops to saline-alkali soil stress and effect of improving saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(6): 1451-1462.
[2] ZHANG Jia-Kang, LI Fei, SHI Shu-De, YANG Hai-Bo. Construction and application of the critical nitrogen concentration dilution model of sugar beet in Inner Mongolia, China [J]. Acta Agronomica Sinica, 2022, 48(2): 488-496.
[3] ZHANG Jian, XIE Tian-Jin, WEI Xiao-Nan, WANG Zong-Kai, LIU Chong-Tao, ZHOU Guang-Sheng, WANG Bo. Estimation of feed rapeseed biomass based on multi-angle oblique imaging technique of unmanned aerial vehicle [J]. Acta Agronomica Sinica, 2021, 47(9): 1816-1823.
[4] WEI Huan-He, ZHANG Xu-Bin, GE Jia-Lin, MENG Tian-Yao, LU Yu, LI Xin-Yue, TAO Yuan, DING En-Hao, CHEN Ying-Long, DAI Qi-Gen. Dynamics in above-ground biomass accumulation after transplanting and its characteristic analysis in Yongyou japonica/indica hybrids [J]. Acta Agronomica Sinica, 2021, 47(3): 546-555.
[5] YU Ning-Ning,ZHANG Ji-Wang,REN Bai-Zhao,ZHAO Bin,LIU Peng. Effect of integrated agronomic managements on leaf growth and endogenous hormone content of summer maize [J]. Acta Agronomica Sinica, 2020, 46(6): 960-967.
[6] YAO Chen-Tao,QIAO Zhi-Hua,SONG Xue-Hui,ZHANG Feng-Wen,SUN Xiao,LI Gang,LI Xiang-Dong,ZHANG Ji-Wang,JIANG Xing-Yin. Effect of different film-forming agents on the persistence and safety of thiacloprid suspension concentrate for seed coating for maize [J]. Acta Agronomica Sinica, 2020, 46(02): 269-279.
[7] YAN Qing-Qing,ZHANG Ju-Song,DAI Jian-Min,DOU Qiao-Qiao. Effects of glycinebetain on photosynthesis and biomass accumulation of island cotton seedlings under saline alkali stress [J]. Acta Agronomica Sinica, 2019, 45(7): 1128-1135.
[8] RU Xiao-Ya,LI Guang,CHEN Guo-Peng,ZHANG Tong-Shuai,YAN Li-Juan. Regulation effects of water and nitrogen on wheat yield and biomass in different precipitation years [J]. Acta Agronomica Sinica, 2019, 45(11): 1725-1734.
[9] Yu TIAN,Lei YANG,Ying-Hui LI,Li-Juan QIU. Development and Utilization of KASP Marker for SCN3-11 Locus Resistant to Soybean Cyst Nematode [J]. Acta Agronomica Sinica, 2018, 44(11): 1600-1611.
[10] Hai-Xia WU,Li-Li GUO,Li-Hua HAO,Hao ZHANG,Qing-Tao WANG,Dong-Juan CHENG,Zheng-Ping PENG,Fei LI,Xi-Xi ZHANG,Shu-Bin LI,Ming XU,Yun-Pu ZHENG. Effects of Water and CO2 Concentration on Stomatal Traits, Leaf Gas Exchange, and Biomass of Winter Wheat [J]. Acta Agronomica Sinica, 2018, 44(10): 1570-1576.
[11] Shen-Bin YANG, Sha-Sha XU, Xiao-Dong JIANG, Chun-Lin SHI, Ying-Ping WANG, Shuang-He SHEN. Correcting the Response of Maximum Leaf Photosynthetic Rate to Temperatures in Crop Models [J]. Acta Agronomica Sinica, 2018, 44(05): 750-761.
[12] CHEN Yu-Li,YANG Ping,ZHANG Wen-Yu,ZHANG Wei-Xin,ZHUYe-Ping,LI Shi-Juan,GONG Fa-Jiang,BI Hai-Bin,YUE Ting,CAO Hong-Xin. Biomass-Based Main Spike MorphologicalParameter Model for Winter Wheat [J]. Acta Agron Sin, 2017, 43(03): 399-406.
[13] TONG Zhi-Jun, JIAO Fang-Chan, FANG Dun-Huang, CHEN Xue-Jun, WU Xing-Fu, ZENG Jian-Min, XIE He, ZHANG Yi-Han, XIAO Bing-Guang*. Construction and Genetic Evaluation of Chromosome Segment Substitution Linesin Tobacco(NicotianatabacumL.) [J]. Acta Agron Sin, 2016, 42(11): 1609-1619.
[14] CHEN Yu-Li,YANG Ping,ZHANG Wen-Yu,ZHANG Wei-Xin,ZHU Ye-Ping,LI Shi-Juan,GONG Fa-Jiang,BI Hai-Bin,YUE Ting,CAO Hong-Xin. Aboveground Architecture ModelBased onBiomass of Winter Wheat before Overwintering [J]. Acta Agron Sin, 2016, 42(05): 743-750.
[15] SU Shi,LI Rui-Hang,LANG Dan-Ying,ZHANG Ke,HAO Xiao-Hu,LIU Yan,WANG Jun-Wei,XU Hong. Microstructure of Glandular Trichomes on Leaf Surface of Sesame and Changes of Trichome Secretions under Drought Condition [J]. Acta Agron Sin, 2016, 42(02): 278-294.
Full text



No Suggested Reading articles found!