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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1625-1630.doi: 10.3724/SP.J.1006.2012.01625


Expression of Rice Defence Genes under Small Brown Planthopper Stress

LI Wan-Chang1,2,**,YU Jiao-Jiao1,2,**,DUAN Can-Xing1,*,ZHU Zhen-Dong1,WANG Xiao-Ming1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Genetic Resources and Improvement, Beijing 10081, China; 2 Henan Normal University, Xinxiang 453007, China
  • Received:2012-02-09 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 段灿星, E-mail: duancx@caas.net.cn, Tel: 010-82109609? ** 同等贡献(Contributed equally to this work)

Abstract: The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacide), is an economically important pest in rice (Oryza sativaL.) production in China.Real-time PCR was used to determine transcriptional level of rice defence genes after SBPH infestation using specific primers. The expression level of SA synthesis-related genes PAL, NPR1, EDS1, and PAD4 was higher in resistant Mudgo than in susceptible Kittake after SBPHfeeding. The expression level of gene PAL in 12 h-infestation rice was 6.914 times of that in untreated rice. The gene PAL accumulation was more rapid and at higher levels in Mudgo and the expression amount in Mudgo was 42.848, 70.743, and 69.193 times of that in Kittake at 24, 48, and 72 h after SBPH infestation, respectively.The gene NPR1 expression in Mudgo was 4.690, 6.231, and 4.112 times of that in Kittake after SBPH infestation for 12, 36, and 72 h. There was significant difference in transcriptional level of the JA synthesis-related genes LOX and AOS2 after 36 h-infestation between Mudgo and Kittake. The expression level was substantially lower in Mudgo than in Kittake at subsequent time points. In addition, the expression level of receptor gene EIN2 in ethylene signaling pathway was higher in Kittake than in Mudgo after SBPHfeeding. The above results indicated that SBPH feeding activatedthe salicylic acid signaling pathway in resistantMudgo and induced the defenses in susceptible Kittake associated with a JA/ethylene-dependent pathway. The genesPAL and NPR1 played a considerable role in the regulation of Mudgo expressing resistanceto SBPH.

Key words: Rice, Small brown Planthopper, Defence genes, Real-time PCR

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