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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1665-1671.doi: 10.3724/SP.J.1006.2012.01665

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Optimal Moisture Content and Physiological Bases of Rice Seeds Storage at Room Temperature

HU Qun-Wen1,2,XIN Xia1,CHEN Xiao-Ling1,LIU Xu1,*,LU Xin-Xiong1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Beijing 100081, China; 2 Anhui Agricultural University, Hefei 230036, China
  • Received:2012-01-09 Revised:2012-06-06 Online:2012-09-12 Published:2012-07-03
  • Contact: 卢新雄, 刘旭, E-mail: xxlu@caas.net.cn, Tel: 010-62174099

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Abstract: The objective of this study was to investigate the optimal moisture content for rice seeds storage and the physiological bases by measuring the seed viability, vigor index, malondialdehyde (MDA) content, leakage electrical conductivity, and antioxidant enzyme activities in the embryo of rice seeds, which had been stored at room temperature in Nanchang for nine years with moisture content (MC) at 10.6%, 7.0%, 6.0%, 5.0%, 4.0%, and 3.0% respectively. The data showed that after nine years storage at room temperature, the germination rates of seeds with MC of 5.0–6.0% were still higher than 50%, while that with other MCs much lower. Therefore the MC of 5.0–6.0% was regarded as the optimal moisture content (MCopt) for rice seed storage at room temperature. The activities of APX and CAT in the 12 h-imbied embryo of nine-year stored seeds with MCopt were much higher than those with other MCs, which showed no or minimal differences with those stored at -18℃. There were no significant changes in the activities of GR or SOD. These results suggested that the MCopt could maintain higher activities of APX and CAT, which might be one of the important reasons for retarding the reduction of seed viability during storage. No clear relationship was detected between seed leakage electrical conductivity or MDA content in embryo and seed viability or moisture content. When the seeds were dried to MC of 3.0%, the over-drying injury was introduced as the markedly decline in germination rate and germination index.

Key words: Rice (Oryza sativa L.), Storage at room temperature, Optimal moisture content, Seed viability, Antioxidase

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