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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 727-734.doi: 10.3724/SP.J.1006.2013.00727

• RESEARCH NOTES • Previous Articles     Next Articles

Development of Specific Molecular Markers for Thinopyrum elongatum Chromosome Using SLAF-seq Technique

CHEN Shi-Qiang,QIN Shu-Wen,HUANG Ze-Feng,DAI Yi,ZHANG Lu-Lu,GAO Ying-Ying,GAO Yong,CHEN Jian-Min*   

  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
  • Received:2012-11-15 Revised:2013-01-16 Online:2013-04-12 Published:2013-01-28
  • Contact: 陈建民, E-mail: jmchen@yzu.edu.cn, Tel: 0514-87979286

Abstract:

The 1E and 7E chromosomes of Thinopyrum elongatum carry important resistance genes to wheat Fusarium head blight. Mapping and utilization of these resistance genes require numerous molecular markers specific to the 1E or 7E chromosome. In this study, we developed 368 specific fragments of Thinopyrum elongatum 1E chromosome using SLAF-seq technique, and randomly selected 80 fragments to design specific primers. Finally, 20 1E-specific, 2 genome-specific, and 26 other specific molecular markers were obtained, with the efficiency up to 60%. All the newly developed markers could amplify the specific bands in different lines derived from wheat–Th. elongatum progenies. According to the cosegregation of the specific markers and elite traits, some markers detected could be closely linked to the genes corresponding to target traits.

Key words: Thinopyrum elongatum, SLAF-seq technique, Chromosome specific molecular marker, Wheat Fusarium head blight, Marker-assisted selection

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