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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (11): 1692-1700.doi: 10.3724/SP.J.1006.2015.01692


Key Components of Eating Quality and their Dynamic Accumulation in Vegetable Soybean Varieties [Glycine max (L.) Merr.]

ZHANG Qiu-Ying1,*,LI Yan-Sheng1,LIU Chang-Kai1,2,TIAN Bo-Wen1,2,TU Bing-Jie1,2,MAO Jian-Wei3   

  1. 1 Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;
    2 College of Natural Resources and Environment, Northeast Agricultural University, Harbin150081, China; 3 Jilin Provincial Agro-tech Extension Center, Changchun 130033, China
  • Received:2015-04-25 Revised:2015-07-20 Online:2015-11-12 Published:2015-08-05
  • Contact: 张秋英, E-mail: zhangqiuying@iga.ac.cn, Tel: 0451-86601320


The eating quality and seed chemical compositions of 30 vegetable soybean cultivars (line), as well as their correlations were analyzed. At the same time, main quality compositions and their roles for eating quality were investigated by path analysis, while the dynamic accumulation of each component was also studied with three cultivars differing in soluble sugar content. Results showed that the cumulative contribution of sucrose, fructose + glucose, raffinose, stachyose, protein, free amino acid and oil for eating quality was 67.8%. A significant positive correlation was found between eating quality score and sucrose content (0.864**), while a significant negative correlation between eating quality score and protein content (–0.439*). The average content of raffinose and stachyose at fresh harvest stage was only 7.4% and 12.4% of that at mature stage respectively, which had much less negative impact on eating quality of vegetable soybean than field grain soybean. The optimum harvest time for fresh pods was at 42 days after flowering and could last for one week with highest sucrose content and lowest raffinose and stachyose contents. Besides, the average content of amino acids was higher and fructose + glucose contents were medium, and protein content was 3% less than that at mature stage. Postponing the harvest time deteriorated eating quality due to decreased content in sucrose and monosaccharide, increased content in fat and protein contents, as well as a dramatic increase in raffinose and stachyose contents.

Key words: Vegetable soybean, Eating quality, Sucrose, Fresh harvest stage

[1]Konovsky J, Lumpkin T A, McClary D. Edamame: the vegetable soybean: In: O'Rourke A D ed. Understanding the Japanese Food and Agrimarket: a multifaceted opportunity. Binghamton: Haworth Press, 1994. pp 73–181

[2]张秋英, 杨文月, 李艳华, 潘相文, Herbert S J. 中国菜用大豆研究现状、生产中的问题及展望. 大豆科学, 2007, 26: 950–954

Zhang Q Y, Yang W Y, Li Y H, Pan X W, Herbert S J. Research, production and prospects for vegetable soybean in China. Soybean Sci, 2007, 26: 950–954 (in Chinese with English abstract)

[3]韩天富, 盖钧镒. 世界菜用大豆生产、贸易和研究的进展. 大豆科学, 2002, 21: 278–284

Han T F, Gai J Y. Advances in production, trade and research of vegetable soybeans in the world. Soybean Sci, 2002, 21: 278–284 (in Chinese with English abstract)

[4]李之国, 张彩英, 常文锁. 不同来源菜用大豆的品质研究. 植物遗传资源学报, 2006, 7: 183–187

Li Z G, Zhang C Y, Chang W S. Quality of vegetable soybeans from different sources. J Plant Genet Resourc, 2006, 7: 183–187 (in Chinese with English abstract)

[5]李彦生, 南海洋, 杜明, 连腾祥, 张秋英, 刘晓冰. 菜用大豆籽粒不同部位蔗糖积累及关键酶活性研究. 作物学报, 2013, 39: 2099–2105

Li Y S, Nan H Y, Du M Lian T X, Zhang Q Y, Liu X B. Sucrose accumulation and key enzymes activities from different tissues of seed in vegetable soybean. Acta Agron Sin, 2013, 39: 2099–2105 (in Chinese with English abstract)

[6]徐兆生, 王素, 魏民. 菜用大豆种质资源园艺性状鉴定和优异资源筛选. 作物品种资源, 1996, (2): 14–16

Xu Z S, Wang S M, Wei M. Trait identifcation and elite germplasm screening for vegetable soybeans. China Seeds, 1996, (2): 14–16 (in Chinese with English abstract)

[7]陈学珍, 谢皓, 郑晓宇, 李婷婷, 白宝良, 于同泉, 路苹. 菜用大豆的农艺及品质性状评价与相关性分析. 北京农学院学报, 2005, 20(1): 23–26

Chen X Z, Xie H, Zheng X Y, Li T T, Bai B L, Yu T Q, Lu P. Evaluation on agronomic and quality traits and their correlation in vegetable soybeans. J Bejing Agric Coll, 2005, 20(1): 23–26 (in Chinese with English abstract)

[8]Masuda R. Quality requirement and improvement of vegetable soybean. Vegetable Soybean: research needs for production and quality improvement, Asian Vegetable Research Development Center, 1991, pp 92–103

[9]王丹英, 汪自强. 菜用大豆食用品质及其与内含物关系研究. 金华职业技术学院学报, 2002, 2(3): 15–17

Wang D Y, Wang Z Q. Editable quality and their chemical compositions in vegetable soybean. J Jinhua Prof Technol Coll, 2002, 2(3): 15–17 (in Chinese with English abstract)

[10]Min Kuo T, Lowell C A, Smith P T. Changes in soluble carbohydrates and enzymic activities in maturing soybean seed tissues. Plant Sci, 1997, 125: 1–11

[11]张玉荣, 周显青, 杨兰兰. 大米食用品质评价方法的研究现状与展望. 中国粮油学报, 2009, 8: 155–160

Zhang Y R, Zhou X Q, Yang L L. Advances and prospects on the evaluation methods of editable quality in rice. J Chin Grain Oil, 2009, 8: 155–160 (in Chinese with English abstract)

[12]Brar G S, Carter T E. Soybean Glycine max (L.) Merrill. In: Kalloo G. ed. Genetic improvement of vegetable crops, California: 1993. pp 427–463

[13]Tsou S C S, Hong T L. Research on vegetable soybean quality in Taiwan. In: Shanmugasundaram S, ed. Vegetable Soybean Research Needs for Production and Quality Improvement. Taiwan, 1991. pp 103–107

[14]Masuda R, Harada K.Enhancement of sweet components in vegetable soybean seeds: Starch degradation during cooking enhance flavor of immature seeds. In: Lump N T, Shanmugasundaram S, eds. Proceedings and Conference Information of the Second International Vegetable Soybean Conference, Washington, 2001. pp 105–108

[15]张圣平, 刘世琦, 谷卫刚. 栽培技术对菜用大豆果荚可溶性糖含量的影响. 山东农业大学学报(自然科学版), 2003, 34(2): 189–192

Zhang S P, Liu S Q, Gu W G, Production practices effects on soluble sugar content of fresh pod seed in vegetable soybean. J Shandong Agric Univ (Nat Sci Edn), 2003, 34(2): 189–192 (in Chinese with English abstract)

[16]曹玉军, 赵宏伟, 王晓慧, 魏雯雯, 张磊, 王立春, 王永军. 施钾对甜玉米产量、品质及蔗糖代谢的影响. 植物营养与肥料学报, 2011, 4: 881–887

Cao Y J, Zhao H W, Wang X H, Wei W W, Zhang L, Wang L C, Wang Y J. Effects of potassium application on yield, quality and sucrose metabolism in sweet corn. J Plant Nutr Fert, 2011, 4: 881–887 (in Chinese with English abstract)

[17]李文生, 杨媛, 石磊, 冯晓元, 张长松, 杨军军. 水果中蔗糖、还原糖、可溶性糖与甜度相关性的研究. 北方园艺, 2012, (1): 58–60

Li W S, Yang Y, Shi L, Feng X Y, Zhang C S, Yang J J. Sucrose, reductive sugar and soluble sugar in relation to fruits sweetness. North Hort, 2012, (1): 58–60 (in Chinese with English abstract)

[18]Li Y S, Du M, Zhang Q Y, Wang G H, Masoud H, Liu X B. Greater differences exist in seed protein, oil, total soluble sugar and sucrose content of vegetable soybean genotypes [Glycine max (L.) Merrill] in Northeast China. Aust J Crop Sci, 2012, 12: 1681–1686

[19]Shanmugasundaram S, Yan M R, Yang R Y. Association between Protein and oil and sugar in vegetable soybean. In: Lumpkin T A, Shanmugasundaram S, eds. 2nd Int. Vegetable Soybean Conf., Washington State Univ., Pullman, 2001. pp 157–160

[20]闫清平, 朱永义. 大米淀粉、蛋白质与其食用品质关系. 粮食与油脂, 2001, (5): 29–32

Yan Q P, Zhu Y Y. Starch, protein and their relation to editable quality in rice. Grain Oil, 2001, (5): 29–32 (in Chinese with English abstract)

[21]Park J K, Kim S S, Kim K O. Effect of milling ratio on sensory properties of cooked rice and on physicochemical properties of milled and cooked rice. Cereal Chem, 2001, 78: 151–156

[22]吴长明, 孙传清, 付秀林, 王象坤, 李自超, 张强. 稻米品质性状与产量性状及籼粳分化度的相互关系研究. 作物学报, 2003, 29: 822–828

Wu C M, Sun C Q, Fu X L, Wang X K, Li Z C, Zhang Q. Quality, yield traits and interation of differentiated degree between indica and japonica rice. Acta Agron Sin, 2003, 29: 822–828 (in Chinese with English abstract)

[23]沈鹏, 罗秋香, 金正勋. 稻米蛋白质与蒸煮食用品质关系研究. 东北农业大学学报, 2003, 4: 378–381

Shen P, Luo Q X, Jin Z X. Relationship between protein and cooking editable quality in rice. J Northeast Agric Univ, 2003, 4: 378–381 (in Chinese with English abstract)

[24]Saldivar X, Wang Y J, Chen P, Hou A. Changes in chemical composition during soybean seed development. Food Chem, 2011, 124: 1369–1375

[25]Kumar V, Rani A, Goyal L. Evaluation of vegetable-type soybean for sucrose, taste-related amino acids, and isoflavones contents. Int J Food Prop, 2011, 14: 1142–1151

[26]Wilcox J R, Shibles R M. Interrelationships among seed quality attributes in soybean. Crop Sci, 2001,41: 11–14

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