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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1261-1272.doi: 10.3724/SP.J.1006.2016.01261


Genetic Identification of a New D1-allelic Mutant and Analysis of Its Gene Function in Rice

WANG Cui-Hong,MA Jian,WANG Shuai,TIAN Peng,QI Chang-Yan,ZHAO Zhi-Chao,WANG Jiu-Lin,WANG Jie,CHENG Zhi-Jun,ZHANG Xin,GUO Xiu-Ping,LEI Cai-Lin*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-03-21 Revised:2016-06-20 Online:2016-09-12 Published:2016-06-27
  • Contact: 雷财林, E-mail: leicailin@caas.cn E-mail:waangch@sina.com
  • Supported by:

    This study was supported by the Special Fund for Agro-scientific Research in the Public Interest Program of China (Grant No. 20120314) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences “Crop Functional Genomics”.


Plant height is one of important traits for rice yield. One genetically stable rice mutant, LTH-m3, was isolated from the cv. Lijiangxintuanheigu (LTH)-derived mutant population by mutagenesis using ethylmethane sulfonate (EMS). LTH-m3 was involved in the pathway of gibberellic acid (GA) and brassinosteroid (BR), and showed no sensitiveness to exogenous GA (GA3) and significantly reduced sensitiveness to exogenous BR (eBL) compared with the wild type. The genetic analysis, gene cloning and transgenic complementary test confirmed that LTH-m3 was a new d1-allelic mutant with small grain and dwarf phenotypes, and a single base was mutated (G2522→A2522) in the functional dwarf gene D1 at the conjunction site of its sixth exon and intron, which caused excision of the sixth exon in mRNA and premature termination of the D1 encoded Gα protein, resulting in mutated phenotypes in the mutant. The further study showed that the D1 mutation caused obvious expression change of some dwarf genes such as SD1 and SLR1 in the mutant, and could affect the GA and BR pathways in their feedback regulations and signaling transductions in plant cells. The mutant overcome the defects of the universally blast-susceptible cv. LTH, such as too tall plant, soft stem and easy lodging, and could be utilized as an improved substitute of LTH in the future rice blast researches. The mutated D1 gene identified from the LTH-m3 mutant may be useful for further study of Gα functions and signaling pathway of GA and BR.

Key words: Rice, Mutant, Dwarf gene, Gene cloning, Gene function

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