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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (11): 1756-1763.doi: 10.3724/SP.J.1006.2019.91010

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of varieties and cultivation conditions on the bioactive substances contents of wheat grain

CHEN Shi-Hao1,LI Zheng-Yang1,CHEN Jia-Lu1,ZHANG Yuan-Qing1,WEI Yu-Ming2,ZHENG You-Liang2,PU Zhi-En1,*()   

  1. 1 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
    2 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
  • Received:2019-01-26 Accepted:2019-06-24 Online:2019-11-12 Published:2019-08-13
  • Contact: Zhi-En PU E-mail:puzhien@sicau.edu.cn
  • Supported by:
    The study was supported by the National Key Research and Development Program of China(2017YFD0100900)


People pay more attention to the bioactive substances because of its antioxidant capability. To investigate the effect of N-application time, nitrogen fertilizer rate, sowing time and P application note on the bioactive substance contents of wheat grain, we analyzed the contents of phytic acid, γ-aminobutyric acid and total phenol in wheat cultivars planted at two locations for two consecutive growth seasons. The genotype contributed the most variations of bioactive substance contents. The cultural practices had less influence than varieties, the interaction between N-application and phosphorus application also had significant effect on bioactive substances contents. Mianmai 51 had the highest contents of γ-aminobutyric and total phenol and the lowest phytic acid content. According to the results, the gains of Mianmai 51 with the optimum content of bioactive substances can be obtained by using the base fertilizer of 150 kg hm -2 of pure nitrogen and 75 kg hm -2 of P2O5 and rowing on October 29th in Sichuan province.

Key words: N-dosage applied, N-applying stages, sowing times, P application, variety, antioxidant

Table 1

Significance test of the effect of N-applying stages on the content of bioactive substances"

Variation source
γ-氨基丁酸 γ-aminobutyric acid 植酸 Phytic acid 总酚 Total phenol
年份 Year 66.015 0.000** 11.971 0.001** 20.850 0.000**
地点 Location 48.469 0.000** 141.058 0.000** 3.079 0.083
施氮时期 N-applying stages (N-S) 0.507 0.604 0.881 0.418 2.301 0.107
品种 Variety (V) 12.443 0.000** 35.200 0.000** 23.278 0.000**
区组 Group (G) 0.475 0.493 0.017 0.897 0.860 0.356
N-S ×G 0.126 0.882 0.038 0.962 0.316 0.730
V × N-S 0.149 0.989 1.361 0.241 1.687 0.135

Table 2

Multiple comparison of bioactive substances contents in varieties of wheat"

γ-aminobutyric acid (mg kg-1)
Phytic acid (mg g-1)
Total phenol (μg g-1)
川农16 Chuannong 16 85.661 c 11.514 a 243.044 b
绵麦51 Mianmai 51 112.754 a 9.311 c 319.132 a
蜀麦969 Shumai 969 98.253 b 10.430 b 257.613 b
蜀麦482 Shumai 482 100.361 b 11.508 a 251.061 b

Table 3

Significance test of the effect of N-dosage applied on bioactive substances"

Variation source
γ-氨基丁酸 γ-aminobutyric acid 植酸 Phytic acid 总酚 Total phenol
年份Year 364.227 0.000** 0.505 0.479 30.858 0.000**
地点Location 164.068 0.000** 0.282 0.597 35.120 0.000**
施氮量N-dosage applied (N) 3.691 0.014* 5.958 0.001** 0.236 0.871
品种 Variety (V) 42.292 0.000** 15.682 0.000** 27.933 0.000**
区组 Group (G) 0.033 0.856 0.012 0.913 0.036 0.850
N×G 0.086 0.968 0.243 0.866 0.882 0.453
V×N 0.795 0.622 1.240 0.279 0.576 0.814

Table 4

Multiple comparison of bioactive substances contents in wheat under different N-dosage applied"

N-dosage applied
γ-aminobutyric acid (mg kg-1)
Phytic acid (mg g-1)
Total phenol (μg g-1)
N0 94.223 b 11.541 a 276.985 a
N1 98.991 ab 11.509 a 283.010 a
N2 97.292 ab 10.684 b 273.258 a
N3 102.444 a 10.825 b 279.759 a

Table 5

Significance test of the effect of the interaction between N-dosage applied and sowing time on bioactive substances"

Variation source
γ-aminobutyric acid
Phytic acid
Total phenol
年份Year 505.081 0.000** 64.828 0.000** 185.745 0.000**
地点Location 22.804 0.000** 35.685 0.000** 74.709 0.000**
品种 Variety (V) 10.772 0.000** 9.529 0.000** 9.429 0.000**
施氮量N-dosage applied (N) 2.258 0.135 14.343 0.000** 2.162 0.143
播期Sowing time (S) 3.351 0.037* 3.118 0.047* 0.089 0.915
N×S 0.279 0.757 1.692 0.187 0.028 0.972
V×N×S 1.830 0.096 0.285 0.943 0.335 0.917

Table 6

Multiple comparison of bioactive substances contents in wheat under different sowing time"

Sowing time
γ-aminobutyric acid (mg kg-1)
Phytic acid (mg g-1)
Total phenol (μg g-1)
B1 104.032 ab 11.057 a 257.868 a
B2 107.435 a 10.525 b 254.179 a
B3 101.438 b 10.666 ab 253.979 a

Table 7

Significance test of the effect of the interaction between N-dosage applied and P application rate on bioactive substances"

Variation source
γ-氨基丁酸 γ-aminobutyric acid 植酸 Phytic acid 总酚 Total phenol
年份Year 2.570 0.111 6.400 0.012 138.680 0.000**
地点Location 0.029 0.866 13.191 0.000** 0.004 0.952
品种 Variety (V) 21.310 0.000** 11.487 0.000** 2.799 0.042*
施氮量N-dosage applied (N) 2.730 0.100 6.283 0.013* 26.317 0.000**
播期Sowing time (S) 1.227 0.296 8.506 0.000** 3.308 0.039*
N×S 0.818 0.443 3.285 0.040* 0.801 0.451
V×N×S 0.801 0.570 1.760 0.110 0.623 0.712

Table 8

Multiple comparison of bioactive substances contents in wheat under different P application rate"

P application rate
γ-aminobutyric acid (mg kg-1)
Phytic acid (mg g-1)
Total phenol (μg g-1)
P0 89.403 a 9.660 b 233.490 b
P1 92.773 a 10.443 a 249.649 a
P2 92.119 a 10.351 a 250.467 a

Table 9

Multiple comparison of bioactive substances contents in wheat under the interaction between N-dosage applied and P application rate"

N-dosage applied ×P application rate
γ-aminobutyric acid (mg kg-1)
Phytic acid (mg g-1)
Total phenol (μg g-1)
N5, P0 89.536 a 9.591 c 223.240 b
N5, P1 90.531 a 10.904 a 230.496 b
N5, P2 89.596 a 10.595 ab 233.097 b
N10, P0 89.262 a 9.728 c 243.739 b
N10, P1 95.005 a 9.982 c 268.803 a
N10, P2 94.633 a 10.106 bc 267.837 a
[1] 赵春, 李增嘉 . 生态环境对小麦品质的影响及小麦生产区域化研究. 安徽农业科学, 2008,36:7610-7611.
Zhao C, Li Z J . Research on the ecological environment on the wheat quality and production. Anhui Agric Sci, 2008,36:7610-7611 (in Chinese with English abstract).
[2] 刘姣, 汪丽萍, 谭斌, 吴卫国 . 小麦麸皮生物加工及其在面制品中应用研究进展. 食品工业科技, 2016,37(12):375-379.
Liu J, Wang L P, Tan B, Wu W G . Research progress in wheat bran bioprocessing and its application in flour products. Sci Technol Food Ind Maga Prof, 2016,37(12):375-379 (in Chinese with English abstract).
[3] 郝家伟, 于素平, 管超 . 小麦品质控制与粮食储存. 粮食储藏, 2004,33(5):39-41.
Hao J W, Yu S P, Guan C . Quality control of wheat and grain storage. Grain Storage, 2004,33(5):39-41 (in Chinese with English abstract).
[4] 杨佳, 侯占群, 贺文浩, 彭强, 袁芳, 高彦祥 . 微胶囊壁材的分类及其性质比较. 食品发酵与工业, 2009,35(5) : 122-126.
Yang J, Hou Z Q, He W H, Peng Q, Yuan F, Gao Y X . Classification and characterization of microencapsulated wall materials. Food Ferment Ind, 2009,35(5):122-126 (in Chinese with English abstract).
[5] 王旭峰, 何计国, 陶纯洁, 单秀峰 . 小麦麸皮的功能成分及加工利用现状. 粮食与食品工业, 2006,13(1):19-22.
Wang X F, He J G, Tao C J, Shan X F . Current of functional ingredients and exploitation of wheat bran. Cereal Food Ind, 2006,13(1):19-22 (in Chinese with English abstract).
[6] Bonilla E, Azuara E, Beristain C I, Vernon-Carter E J . Predicting suitable storage conditions for spray-dried microcapsules formed with different biopolymer matrices. Food Hydrocolloids, 2010,24:633-640.
[7] Baranauskiene R, Bylaite E, Zukauskaite J, Venskutonis R . Flavor retention of peppermint (Mentha piperita L.) essential oil spray-dried in modified starches during encapsulation and storage. J Agric Food Chem, 2007,55:3027-3036.
[8] 江生, 吴向阳, 仰榴青, 邹艳敏 . 小麦麸皮不同提取物清除自由基的作用. 食品研究与开发, 2009,30(6):27-30.
Jiang S, Wu X Y, Yang L Q, Zou Y M . Wheat bran different extract on the effect of free radical scavenging. Food Res Dev, 2009,30(6):27-30 (in Chinese with English abstract).
[9] Xu G H, Ye X Q, Chen J C, Liu D H . Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract. J Agric Food Chem, 2007,55:330-335.
[10] Oh B C, Choi W C, Park S, Kim Y O, Oh T K . Biochemical properties and substrate specificities of alkaline and histidine acid phytases. Appl Microbiol Biotechnol, 2004,63:362-372.
[11] 姚瑾, 李先德 . 从小麦营养品质看中国的小麦与加工业发展. 中国乳业, 1999, ( 9):6-8.
Yao J, Li X D . Looking at the development of Chinese wheat and processing industry from the nutritional quality of wheat. China Dairy, 1999, ( 9):6-8 (in Chinese with English abstract).
[12] 石书兵, 马林, 石庆华, 刘霞, 陈乐梅, 刘建喜, 王振林 . 不同施氮时期对冬小麦子粒蛋白质组分及其动态变化的影响. 植物营养与肥料学报, 2005,11:456-460.
Shi S B, Ma L, Shi Q H, Liu X, Chen L M, Liu J X, Wang Z L . Effect of nitrogen application timing on protein constitutes and its dynamic change in wheat grain. Plant Nutr Fert Sci, 2005,11:456-460 (in Chinese with English abstract).
[13] 李姗姗, 赵广才, 常旭虹, 刘利华, 杨玉双, 丰明 . 追氮时期对不同粒色类型小麦产量和品质的影响. 植物营养与肥料学报, 2009,15:255-260.
Li S S, Zhao G C, Chang X H, Liu L H, Yang Y S, Feng M . Effects of nitrogen top dressing time on yield and quality in wheat with different types of grain color. Plant Nutr Fert Sci, 2009,15:255-260 (in Chinese with English abstract).
[14] 王月福, 于振文, 李尚霞, 余松烈 . 土壤肥力和施氮量对小麦氮素吸收运转及产量和蛋白质含量的影响. 应用生态学报, 2003,14:1868-1872.
Wang Y F, Yu Z W, Li S X, Yu S L . Effects of soil fertility and nitrogen application rate on nitrogen absorption and translocation, grain yield, and grain protein content of wheat. Chin J Appl Ecol, 2003,14:1868-1872 (in Chinese with English abstract).
[15] 赵宁春, 张小明, 叶胜海, 程方民 . 不同栽培方式和施氮量对稻米营养品质及植酸积累的影响. 浙江农业学报, 2009,21:259-263.
Zhao N C, Zhang X M, Ye S H, Cheng F M . Effects of different cultivation methods and nitrogen application on grain phytic acid contents and nutritional quality for japonica rice. Acta Agric Zhejiangensis, 2009,21:259-263 (in Chinese with English abstract).
[16] Zuo Y, Ma D Y, Wang C Y, Zhu Y J, Liu J, Guo T C . Effects of spraying nitrogen and zinc fertilizers after flowering on grain weight and nutritional quality of winter wheat. Agric Sci & Technol, 2013,33:630-634.
[17] Yu L, Perret J, Harris M, Wilson J, Haley S . Antioxidant properties of bran extracts from “Akron” wheat grown at different locations. J Agric Food Chem, 2003,51:1566-1570.
[18] 时侠清, 沙超, 姚维东, 王超 . 双吡啶分光光度法测定小麦植酸含量研究. 安徽科技学院学报, 2009, ( 6):10-14.
Shi X Q, Sha C, Yao W D, Wang C . Study on bipyridine colorimetric method for determination of wheat phytic acid content. J Anhui Sci Technol Univ, 2009, ( 6):10-14 (in Chinese with English abstract).
[19] 赵大伟, 普晓英, 曾亚文, 李本逊, 杜娟, 杨树明 . 大麦籽粒γ-氨基丁酸含量的测定分析. 麦类作物学报, 2009,29:69-72.
Zhao D W, Pu X Y, Zeng Y W, Li B X, Du J, Yang S M . Determination of the γ-aminobutyric Acid in Barley. J Triticeae Crops, 2009,29:69-72 (in Chinese with English abstract).
[20] Moore J, Liu J G, Zhou K Q, Yu L L . Effects of genotype and environment on the antioxidant properties of hard winter wheat bran. J Agric Food Chem, 2006,54:5313-5322.
[21] 吕俊丽 . 美国马里兰不同品种小麦抗氧化成分分析与功能性评价. 西北农林科技大学博士学位论文,陕西杨凌, 2013.
Lyu J L . Antioxidant Component Analysis and Functional Evaluation of Different Genotype Wheat in Maryland. PhD Dissertation of Northwest A&F University, Yangling, Shaanxi,China, 2013 (in Chinese with English abstract).
[22] 刘茜茜 . 四川不同小麦品种的生物活性物质及抗氧化特性的基因型与环境及其互作效应分析. 四川农业大学硕士学位论文,四川雅安, 2015.
Liu Q Q . The Effects of Genotype, Environment and Their Interaction on the Bioactive Substances and Antioxidant Properties of Different Wheat Varieties in Sichuan Province. MS Thesis of Sichuan Agricultural University, Ya’an, Sichuan, China, 2015 (in Chinese with English abstract).
[23] 孙德祥, 马冬云, 王晨阳, 李耀光, 刘卫星, 李秋霞, 冯伟, 郭天财 . 不同水氮处理对豫麦49-198抗氧化物含量的影响. 作物学报, 2014,40:2046-2051.
Sun D X, Ma D Y, Wang C Y, Li Y G, Liu W X, Li Q X, Feng W, Guo T C . Effects of irrigation and nitrogen on antioxidant contents in Yumai 49-198 grains. Acta Agron Sin, 2014,40:2046-2051 (in Chinese with English abstract).
[24] 朱伟锋, 林咸永, 金崇伟, 章永松, 方萍 . 氮肥对不同白菜品种抗氧化物质含量和抗氧化活性的影响. 浙江大学学报(农业与生命科学版), 2009,35:299-306.
Zhu W F, Lin X Y, Jin C W, Zhang Y S, Fang P . Effects of nitrogen application rates on antioxidant contents and antioxidative activities in Chinese cabbage. J Zhejiang Univ (Agric Life Sci), 2009,35:299-306 (in Chinese with English abstract).
[25] 胡安新, 胡春艳 . 磷肥用量对优质弱筋小麦产量及品质的影响. 农业工程技术, 2018, ( 17):17-18.
Hu A X, Hu C Y . Effect of phosphorus fertilizer on yield and quality of high-quality weak gluten wheat. Appl Eng Technol, 2018, ( 17):17-18 (in Chinese with English abstract).
[26] Lyu J L, Lu Y J, Niu Y G . Effect of genotype, environment, and their interaction on phytochemical compositions and antioxidant properties of soft winter wheat flour. Food Chem, 2013,138:454-462.
[27] Oatway L, Vasanthan T, Helm H . Phytic acid. Food Rev Int, 2001,17:419-431.
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