Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (12): 3120-3129.doi: 10.3724/SP.J.1006.2022.12077

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic analysis and gene mapping of the yellow midrib leaf mutant (yml) in rice (Oryza sativa L.)

GUO Jun-Yao1,2(), LIU Bin-Mei1,2, YANG Hui-Jie1,2, QIN Chao-Qi2, REN Yan2, JIANG Hong-Rui2, TAO Liang-Zhi2, YE Ya-Feng2, WU Yue-Jin1,2()   

  1. 1Institutes of Physical Science and Information Technology, Anhui University, Hefei 230031, Anhui, China
    2Hefei Institutes of Physical Science, Chinese Academy of Science, Hefei 230031, Anhui, China
  • Received:2021-11-06 Accepted:2022-03-25 Online:2022-12-12 Published:2022-04-19
  • Contact: WU Yue-Jin E-mail:154519697@qq.com;yjwu@ipp.ac.cn
  • Supported by:
    Natural Science Foundation of Anhui Province(2108085MC99);Hefei Science and Technology Project(J2020G45);Anhui Science and Technology Major Project(202003c08020006)

RichHTML405

15

PDF

793

Abstract:

Rice (Oryza sativa L.) is an important food crop, and its yield has been concerned for a long time. Rice leaves are the essential sites for photosynthesis, and leaf midrib is the tissue with the function of supporting and transporting. Meanwhile, the photosynthetic pigments present in leaf midrib can also provide a certain amount of photosynthesis. In this study, the yellow midrib leaf (yml) mutant obtained by the heavy ion beam implantation on indica rice 9311 was applied for genetic analysis and gene mapping. The mutant began to had the yellowing phenotype of leaf midrib about 5 days after flowering at heading stage, and the yellowing character was obvious at the late heading stage, and this change could last until the mature stage. At tillering stage, there was no significant difference in photosynthetic pigment content between the mutant and the wild type. However, compared with wild type at the late heading stage, the photosynthetic pigment content in the leaves and midribs of the mutant was significantly lower. Consequently, the photosynthetic efficiency of the mutant was substantially reduced, and the net photosynthetic rate was only 50.37% of wild type. At mature stage, plant height, panicle length, effective panicle, filled grain number per panicle, seed setting rate, and the 1000-grain weight of mutants were significantly lower than wild type. Genetic analysis revealed that this mutant character was controlled by a pair of recessive genes. By using map-based cloning technique, this gene was located on chromosome 6. Moreover, the gene was further located between InDel5 and RM3431 with a physical distance of approximately 700 kb by using simple repeat sequence (SSR) and insertional deletion (InDel) markers. This study provides a research basis for the subsequent cloning and functional analyses of the mutant gene.

Key words: Oryza sativa L., midrib, yellow, photosynthetic, gene mapping

Fig. 1

Phenotypic characteristics of wild type (9311) and mutant yml A: plants of wild-type (9311) and yml mutant at heading stage; Bar: 10 cm; B: flag leaf of wild-type (9311) and yml mutant at heading stage; Bar: 5 cm; C: panicle of wild-type (9311) and yml mutant; Bar: 5 cm."

Table 1

Agronomic traits of wild type (9311) and mutant yml"

材料
Material name		 株高
Plant height
(cm)		 穗长
Panicle length
(cm)		 有效穗数
Effective
panicle		 每穗实粒数
Filled grain number
per panicle		 结实率
Seed setting rate (%)		 千粒重
1000-grain weight (g)
9311 127.33±4.29 22.42±2.17 9.67±1.53 158.62±27.98 71.16±9.55 28.06±2.16
yml 97.41±4.15** 20.67±2.79* 4.67±0.58** 105.00±30.58** 43.20±9.02** 23.10±0.41**

Fig. 2

Contents and ratios of various photosynthetic pigments in leaves and midrib of wild type (9311) and mutant yml at different stages A: the content of various photosynthetic pigments in leaves of wild type (9311) and mutant yml at tillering stage; B: the content of various photosynthetic pigments in midrib of wild type (9311) and mutant yml at tillering stage; C: the content of various photosynthetic pigments in leaves of wild type (9311) and mutant yml at heading stage; D: the content of various photosynthetic pigments in midrib of wild type (9311) and mutant yml at heading stage; E: the ratio of carotenoids to total chlorophyll in leaves of wild type (9311) and mutant yml at tillering stage; F: the ratio of carotenoids to total chlorophyll in midrib of wild type (9311) and mutant yml at heading stage. *: P < 0.05; **: P < 0.01."

Table 2

Photosynthetic characteristics of wild type (9311) and mutant yml"

材料
Material		 净光合速率
Photosynthetic rate
(μmol CO2 m-2 s-1)		 蒸腾速率
Transpiration rate
(mol H2O m-2 s-1)		 气孔导度
Stomatal conductance
(mmol H2O m-2 s-1)		 胞间二氧化碳浓度
Intercellular CO2 concentration
(μmol CO2 L-1)
9311 14.95 ±0.74 3.96±0.33 279.97±3.72 338.00±7.21
yml 7.53±0.15** 5.96±0.34** 227.47±15.88** 390.00±9.54**

Fig. 3

Electrophoresis diagram of initial location of yml genes M: 9311; W: japonica var. Nipponbare; M: DL2000 marker."

Fig. 4

Linkage map of yml genes on chromosome 6 in rice"

Table 3

New developed polymorphic InDel makers based on the genome sequence differences between indica var. 9311 and japonica var. Nipponbare"

引物名称
Prime name		 引物序列
Prime sequence
(5'-3')		 物理位置
Physical position
(bp)		 9311 PCR
产物大小
PCR product size of 9311
(bp)		 日本晴PCR
产物大小
PCR product size of Nipponbare
(bp)		 中花11 PCR
产物大小
PCR product size of Zhonghua 11 (bp)
InDel 3 F: CTGGCGGTCGAATTTCTTTA
R: GGCAAATTTCAGCCACAGAT		 7,245,119-7,245,342 160 124 124
InDel 5 F: CAATCAGAATCTGCTTGCCA
R: ACACACATGGCAGGTCACAT		 8,059,757-8,059,997 255 241 241
InDel 13 F: CAGCCACACATTTGGAACCA
R: CTGCAAAGGATCTTGATCCG		 7,813,844-7,814,076 206 233 233

Table 4

Related genes and their putative functions in the target regions"

基因名称
Gene name		 推测功能
Putative function
LOC_Os06g14420 Hydrolase, NUDIX family, domain containing protein, expressed
LOC_Os06g14650 Zinc finger, C3HC4 type domain containing protein, expressed
LOC_Os06g14670 ODORANT1, putative, expressed
LOC_Os06g14700 Myb-like DNA-binding domain containing protein, expressed
LOC_Os06g14710 Myb-like DNA-binding domain containing protein, expressed
LOC_Os06g14750 Phosphatidylinositol-4-phosphate 5-kinase family protein, putative, expressed
LOC_Os06g15380 Acylphosphatase, putative, expressed
LOC_Os06g15390 Acylphosphatase, putative, expressed
LOC_Os06g15410 Angel, putative, expressed
[1] Sheehy J E, Ferrer A B, Mitchell P L, Elmido-Mabilangan A, Pablico P, Dionora M. How the rice crop works and why it needs a new engine. In: In: Sheehy J E, Mitchell P L, Hardy B, eds. Charting New Pathways to C4 Rice. Singapore: World Scientific Publishing Company, 2008. pp 3-26.
[2] 欧阳杰, 王楚桃, 何光华, 钟世良, 李顺武, 李贤勇. 水稻灌浆中后期功能叶中叶绿素含量及其变化趋势与谷物产量关系研究. 西南农业学报, 2012, 25: 1201-1204.
Ou-Yang J, Wang C T, He G H, Zhong S L, Li S W, Li X Y. Study on relationship between different functional leaf chlorophyl Ⅱ content and its trends in mid and late period of rice filling and grain yield. Southwest China J Agric Sci, 2012, 25: 1201-1204. (in Chinese with English abstract)
[3] 杨宙, 黄仁良, 何虎, 刘建华, 曹丰生. 一个新的水稻无中叶脉突变体的表型分析和基因定位. 江西农业学报, 2020, 32(2): 24-28.
Yang Z, Huang R L, He H, Liu J H, Cao F S. Phenotypic analysis and gene mapping of new midrib-deficient mutant in rice. Acta Agric Jiangxi, 2020, 32(2): 24-28 (in Chinese with English abstract).
[4] 郑梦迪, 王春阳, 张寒, 张彦, 汪兴军. 植物叶缘和叶脉发育调控的研究进展. 生物资源, 2019, 41(1): 22-27.
Zheng M D, Wang C Y, Zhang H, Zhang Y, Wang X J. Advances in plant leaf margin and venation pattern regulation mechanism. Biotic Res, 2019, 41(1): 22-27. (in Chinese with English abstract)
[5] 徐敏丽. 水稻两优培九中脉光合特性及蛋白质差异分析. 南京师范大学硕士学位论文, 江苏南京, 2017.
Xu M L. Analysis of Photosynthetic Properties and Protein Differences in Mid-vein of Rice LYP9. MS Thesis of Nanjing Normal University, Nanjing, Jiangsu, China, 2017. (in Chinese with English abstract)
[6] 沈唯军. 水稻中脉中特殊的光合作用和强光响应信号系统. 南京师范大学博士学位论文, 江苏南京, 2014.
Shen W J. Specific Photosynthetic and Intense Light Response Signaling Systems in Mid-vein of Rice (Oryza sativa). PhD Dissertation of Nanjing Normal University, Nanjing, Jiangsu, China, 2014. (in Chinese with English abstract)
[7] 李君霞, 秦娜, 朱灿灿, 王春义, 代书桃, 宋迎辉, 陈宇翔. 谷子黄叶色突变体光合特性研究. 核农学报, 2021, 35: 1964-1970.
doi: 10.11869/j.issn.100-8551.2021.09.1964
Li J X, Qin N, Zhu C C, Wang C Y, Dai S T, Song Y H, Chen Y X. Study on photosynthetic characteristics of foxtail millet mutant with yellow leaf colour. Acta Agric Nucl Sin, 2021, 35: 1964-1970. (in Chinese with English abstract)
[8] Zhang H, Liu L L, Cai M H, Zhu S S, Zhao J Y, Zheng T H, Xu X Y, Zeng Z Q, Niu J, Jiang L, Chen S H, Wan J M. A point mutation of magnesium chelatase OsCHLI gene dampens the interaction between CHLI and CHLD subunits in rice. Plant Mol Biol Rep, 2015, 33: 1975-1987.
doi: 10.1007/s11105-015-0889-3
[9] 简磊, 王仲康, 曾冬冬, 秦冉, 石春海, 金晓丽. 水稻白化转绿突变体albg的鉴定和基因精细定位. 核农学报, 2017, 31: 2289-2297.
doi: 10.11869/j.issn.100-8551.2017.12.2289
Jian L, Wang Z K, Zeng D D, Qin R, Shi C H, Jin X L. Characterization and fine mapping of a green-revertible albino (albg) mutant in rice. Acta Agric Nucl Sin, 2017, 31: 2289-2297. (in Chinese with English abstract)
[10] Zhao M H, Li X, Zhang X X, Zhang H, Zhang X Y. Mutation mechanism of leaf color in plants: a review. Forests, 2020, 11: 851.
doi: 10.3390/f11080851
[11] Awan M A, Konzak C F, Rutger J N, Nilan R A. Mutagenic effects of sodium azide in rice. Crop Sci, 1980, 20: 663-668.
doi: 10.2135/cropsci1980.0011183X002000050030x
[12] Falbel T G, Staehelin L A. Partial blocks in the early steps of the chlorophyll synthesis pathway: a common feature of chlorophyll b-deficient mutants. Physiol Plant, 1996, 97: 311-320.
doi: 10.1034/j.1399-3054.1996.970214.x
[13] Sheng P, Tan J, Jin M, Wu F, Zhou K, Ma W, Heng Y, Wang J, Guo X, Zhang X. Albino midrib 1, encoding a putative potassium efflux antiporter, affects chloroplast development and drought tolerance in rice. Plant Cell Rep, 2014, 33: 1581-1594.
doi: 10.1007/s00299-014-1639-y pmid: 24917171
[14] Wang L, Xu J, Nian J Q, Shen N W, Lai K K, Hu J, Zeng D L, Ge C W, Fang Y X, Zhu L, Qian Q, Zhang G H. Characterization and fine mapping of the rice gene OsARVL4 regulating leaf morphology and leaf vein development. J Plant Growth Regul, 2016, 78: 345-356.
[15] Kusumi K, Mizutani A, Nishimura M, Iba K. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12: 1241-1250.
doi: 10.1046/j.1365-313x.1997.12061241.x
[16] Kusumi K, Sakata C, Nakamura T, Kawasaki S, Yoshimura A, Iba K. A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions. Plant J, 2011, 68: 1039-1050.
doi: 10.1111/j.1365-313X.2011.04755.x
[17] 李红昌, 钱前, 王赟, 李晓波, 朱立煌, 徐吉臣. 水稻白穗突变体基因的鉴定和染色体定位. 科学通报, 2003, 48: 268-270.
Li H C, Qian Q, Wang Y, Li X B, Zhu L H, Xu J C. Identification and chromosomal localization of white spike mutant genes in rice. Chin Sci Bull, 2003, 48: 268-270 (in Chinese with English abstract).
doi: 10.1360/csb2003-48-3-268
[18] 李娜, 储黄伟, 文铁桥, 张大兵. 水稻白色中脉Oswm突变体的遗传分析与基因定位. 上海农业学报, 2007, 23(1): 1-4.
Li N, Chu H W, Wen T Q, Zhang D B. Genetic analysis and mapping of rice white midrib mutant Oswm. Acta Agric Shanghai, 2007, 23(1): 1-4. (in Chinese with English abstract)
[19] 胡景涛, 张甲, 李园园, 付崇允, 郑静, 陈家彬, 胡燕, 李仕贵. 水稻白色中脉Oswm2的遗传分析与分子标记定位. 遗传, 2008, 30: 1201-1206.
Hu J T, Zhang J, Li Y Y, Fu C Y, Zheng J, Chen J B, Hu Y, Li S G. Genetic analysis and mapping of a rice white midrib mutant Oswm2. Hereditas, 2008, 30: 1201-1206. (in Chinese with English abstract)
[20] 朱小燕, 徐芳芳, 桑贤春, 蒋钰东, 代高猛, 王楠, 张长伟, 何光华. 水稻叶脉白化突变体wpsm的遗传分析与基因定位. 作物学报, 2013, 39: 1409-1415.
Zhu X Y, Xu F F, Sang Y C, Jiang Y D, Dai G M, Wang N, Zhang C W, He G H. Genetic analysis and gene mapping of a rice white midrib mutant wpsm. Acta Agron Sin, 2013, 39: 1409-1415. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2013.01409
[21] Akhter D, Qin R, Nath U K, Alamin M, Jin X L, Shi C H. The brown midrib leaf (bml) mutation in rice (Oryza sativa L.) causes premature leaf senescence and the induction of defense responses. Genes, 2018, 9: 203.
doi: 10.3390/genes9040203
[22] Sattler S E, Funnell-Harris D L, Pedersen J F. Brown midrib mutations and their importance to the utilization of maize, sorghum, and pearl millet lignocellulosic tissues. Plant Sci, 2010, 178: 229-238.
doi: 10.1016/j.plantsci.2010.01.001
[23] 杨敏文. 化学科技活动一则: 分光光度法测定叶片叶绿素a、叶绿素b和类胡萝卜素含量. 化学教学, 2002, (8): 44-45.
Yang M W. A chemical technology activity: Spectrophotometric determination of chlorophyll a, chlorophyll b and carotenoids in leaves. Edu Chem, 2002, (8): 44-45. (in Chinese)
[24] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321-4326.
pmid: 7433111
[25] Kraszewska E. The plant Nudix hydrolase family. Acta Biochim Pol, 2008, 55: 663-671.
pmid: 19081844
[26] Shimada H, Koizumi M, Kuroki K, Mochizuki M, Fujimoto H, Ohta H, Masuda T, Takamiya K. ARC3, a chloroplast division factor, is a chimera of prokaryotic FtsZ and part of eukaryotic phosphatidylinositol-4-phosphate 5-kinase. Plant Cell Physiol, 2004, 45: 960-967.
pmid: 15356321
[27] Kong W Y, Yu X W, Chen H Y, Liu L L, Xiao Y J, Wang Y L, Wang C L, Lin Y, Yu Y, Wang C M, Jiang L, Zhai H Q, Zhao Z G, Wan J M. The catalytic subunit of magnesium-protoporphyrin Ⅸ monomethyl ester cyclase forms a chloroplast complex to regulate chlorophyll biosynthesis in rice. Plant Mol Biol, 2016, 92: 177-191.
doi: 10.1007/s11103-016-0513-4
[28] 胡彬华, 王平, 杜安平, 李辉, 王闵霞, 白玉露, 冀占东, 蒲志刚. 水稻淡黄叶突变体pyl3的鉴定和基因定位. 核农学报, 2021, 35: 2696-2703.
doi: 10.11869/j.issn.100-8551.2021.12.2696
Hu B H, Wang P, Du A P, Li H, Wang M X, Bai Y L, Ji Z D, Pu Z G. Characterization and gene mapping of pyl3 mutant in rice. Acta Agric Nucl Sin, 2021, 35: 2696-2703. (in Chinese with English abstract)
[29] 施勇烽, 贺彦, 郭丹, 吕向光, 黄奇娜, 吴建利. 水稻淡绿叶突变体HM133的遗传分析与基因定位. 中国水稻科学, 2016, 30: 603-610.
doi: 10.16819/j.1001-7216.2016.6033
Shi Y F, He Y, Guo D, Lyu X G, Huang Q N, Wu J L. Genetic analysis and gene mapping of a pale green leaf mutant HM133 in rice. Chin J Rice Sci, 2016, 30: 603-610. (in Chinese with English abstract)
[30] Pogson B J, Albrecht V. Genetic dissection of chloroplast biogenesis and development: an overview. Plant Physiol, 2011, 155: 1545-1551.
doi: 10.1104/pp.110.170365 pmid: 21330494
[31] Mei J, Li F, Liu X, Hu G, Fu Y, Liu W. Newly identified CSP41b gene localized in chloroplasts affects leaf color in rice. Plant Sci, 2017, 256: 39-45.
doi: 10.1016/j.plantsci.2016.12.005
[32] Busch M, Seuter A, Hain R. Functional analysis of the early steps of carotenoid biosynthesis in tobacco. Plant Physiol, 2002, 128: 439-453.
pmid: 11842148
[33] Petroni K, Tonelli C. Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci, 2011, 181: 219-229.
doi: 10.1016/j.plantsci.2011.05.009 pmid: 21763532
[34] Holton T A, Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell, 1995, 7: 1071-1083.
doi: 10.2307/3870058
[35] Gu C, Liao L, Zhou H, Wang L, Deng X, Han Y P. Constitutive activation of an anthocyanin regulatory gene PcMYB10.6 is related to red coloration in Purple-Foliage Plum. PLoS One, 2015, 10: e0135159.
[36] 杨颜榕, 黄纤纤, 赵亚男, 汤佳玉, 刘喜. 水稻叶色基因克隆与分子机制研究进展. 植物遗传资源学报, 2020, 21: 794-803.
Yang Y R, Huang Q Q, Zhao Y N, Tang J Y, Liu X. Advances on gene isolation and molecular mechanism of rice leaf color genes. J Plant Genet Res, 2020, 21: 794-803. (in Chinese with English abstract)
[37] Liu X, Huang Q Q, Yang Y R, Tang J, Zhang J. Characterization and map-based cloning of the novel rice yellow leaf mutant yl3. J Plant Biol, 2021, 64: 35-44.
doi: 10.1007/s12374-020-09275-1
[38] Chen D, Qiu Z N, He L, Hou L L, Li M, Zhang G H, Wang X Q, Chen G, Hu J, Gao Z Y, Dong G J, Ren D Y, Shen L, Zhang Q, Guo L B, Qian Q, Zeng D, Zhu L. The rice LRR-like1 protein yellow and premature dwarf 1 is involved in leaf senescence induced by high light. J Exp Bot, 2021, 72: 1589-1605.
doi: 10.1093/jxb/eraa532 pmid: 33200773
[39] Zhu Y, Yan P, Dong S L, Hu Z J, Wang Y, Yang J S, Xin X Y, Luo X J. Map-based cloning and characterization of YGL22, a new yellow-green leaf gene in rice (Oryza sativa). Crop Sci, 2021, 61: 529-538.
doi: 10.1002/csc2.20347
[40] Yu N, Liu Q, Zhang Y, Zeng B, Chen Y Y, Cao Y R, Zhang Y, Rani M H, Cheng S H, Cao L Y. CS3, a Ycf54 domain- containing protein, affects chlorophyll biosynthesis in rice (Oryza sativa L.). Plant Sci, 2019, 283: 11-22.
doi: 10.1016/j.plantsci.2019.01.022
[1] ZOU Xiao-Xia, LIN Yi-Min, ZHAO Ya-Fei, LIU Yan, LIU Juan, WANG Yue-Fu, WANG Wei-Hua. Effects of calcium application on the distribution of photosynthetic carbon in plant-soil system at different peanut pod development stages [J]. Acta Agronomica Sinica, 2023, 49(1): 239-248.
[2] WANG Hai-Qi, WANG Rong-Rong, JIANG Gui-Ying, YIN hao-Jie, YAN Shi-Jie, CHE Zi-Qiang. Effect of amount of nitrogen fertilizer applied on photosynthetic physiological characteristics of drip irrigated spring wheat leaves [J]. Acta Agronomica Sinica, 2023, 49(1): 211-224.
[3] CAO Ji-Ling, ZENG Qing, ZHU Jian-Guo. Responses of photosynthetic characteristics and gene expression in different wheat cultivars to elevated ozone concentration at grain filling stage [J]. Acta Agronomica Sinica, 2022, 48(9): 2339-2350.
[4] GUO Yao, CHAI Qiang, YIN Wen, FAN Hong. Research progress of photosynthetic physiological mechanism and approaches to application in dense planting maize [J]. Acta Agronomica Sinica, 2022, 48(8): 1871-1883.
[5] PEI Li-Zhen, CHEN Yuan-Xue, ZHANG Wen-Wen, XIAO Hua, ZHANG Sen, ZHOU Yuan, XU Kai-Wei. Effects of organic material returned on photosynthetic performance and nitrogen metabolism of ear leaf in summer maize [J]. Acta Agronomica Sinica, 2022, 48(8): 2115-2124.
[6] WEI Gang, CHEN Dan-Yang, REN De-Yong, YANG Hong-Xia, WU Jing-Wen, FENG Ping, WANG Nan. Identification and gene mapping of slender stem mutant sr10 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(8): 2125-2133.
[7] DU Qi-Di, GUO Hui-Jun, XIONG Hong-Chun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, SONG Xi-Yun, LIU Lu-Xiang. Gene mapping of apical spikelet degeneration mutant asd1 in wheat [J]. Acta Agronomica Sinica, 2022, 48(8): 1905-1913.
[8] HUANG Fu-Deng, HUANG Yan, JIN Ze-Yan, HE Huan-Huan, LI Chun-Shou, CHENG Fang-Min, PAN Gang. Physiological characters and gene mapping of a precocious leaf senescence mutant ospls7 in rice (Orzo sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(7): 1832-1842.
[9] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[10] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[11] XU Tian-Jun, ZHANG Yong, ZHAO Jiu-Ran, WANG Rong-Huan, LYU Tian-Fang, LIU Yue-E, CAI Wan-Tao, LIU Hong-Wei, CHEN Chuan-Yong, WANG Yuan-Dong. Canopy structure, photosynthesis, grain filling, and dehydration characteristics of maize varieties suitable for grain mechanical harvesting [J]. Acta Agronomica Sinica, 2022, 48(6): 1526-1536.
[12] ZHANG Yu-Kun, LU Ying, CUI Kan, XIA Shi-Tou, LIU Zhong-Song. Allelic variation and geographical distribution of TT8 for seed color in Brassica juncea Czern. et Coss. [J]. Acta Agronomica Sinica, 2022, 48(6): 1325-1332.
[13] SHI Yan-Yan, MA Zhi-Hua, WU Chun-Hua, ZHOU Yong-Jin, LI Rong. Effects of ridge tillage with film mulching in furrow on photosynthetic characteristics of potato and yield formation in dryland farming [J]. Acta Agronomica Sinica, 2022, 48(5): 1288-1297.
[14] WANG Hao-Rang, ZHANG Yong, YU Chun-Miao, DONG Quan-Zhong, LI Wei-Wei, HU Kai-Feng, ZHANG Ming-Ming, XUE Hong, YANG Meng-Ping, SONG Ji-Ling, WANG Lei, YANG Xing-Yong, QIU Li-Juan. Fine mapping of yellow-green leaf gene (ygl2) in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(4): 791-800.
[15] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Li Shaoqing, Li Yangsheng, Wu Fushun, Liao Jianglin, Li Damo. Optimum Fertilization and Its Corresponding Mechanism under Complete Submergence at Booting Stage in Rice[J]. Acta Agronomica Sinica, 2002, 28(01): 115 -120 .
[2] Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[3] YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[4] Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[5] Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[6] WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[7] TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[8] HU Xi-Yuan;LI Jian-Ping;SONG Xi-Fang. Efficiency of Spatial Statistical Analysis in Superior Genotype Selection of Plant Breeding[J]. Acta Agron Sin, 2008, 34(03): 412 -417 .
[9] WANG Yan;QIU Li-Ming;XIE Wen-Juan;HUANG Wei;YE Feng;ZHANG Fu-Chun;MA Ji. Cold Tolerance of Transgenic Tobacco Carrying Gene Encoding Insect Antifreeze Protein[J]. Acta Agron Sin, 2008, 34(03): 397 -402 .
[10] ZHENG Xi;WU Jian-Guo;LOU Xiang-Yang;XU Hai-Ming;SHI Chun-Hai. Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments[J]. Acta Agron Sin, 2008, 34(03): 369 -375 .
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd