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Screening and validation of OsCYP22 interacting proteins in rice

ZHANG Xiao-Fang1,2,ZHU Qi1,2,HUA Yun-Yan1,2,JIA Li-Hui-Ying1,2,QIU Shi-You1,CHEN Yu-Jie1,MA Tao1,*,DING Wo-Na1,*   

  1. 1 College of Science and Technology / Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, Ningbo University, Ningbo 315300, Zhejiang, China; 2 School of Marine Science, Ningbo University, Ningbo 315211, Zhejiang, China 
  • Received:2023-09-27 Revised:2024-01-31 Accepted:2024-01-31 Published:2024-02-21
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (32071981) and the Ningbo Natural Science Foundation (202003N4016).

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Abstract:

In order to understand the function of cyclophilin family OsCYP22 from Oryza sativa L., the yeast two hybrid bait vector of OsCYP22 was constructed and proteins interacting with OsCYP22 were screened by yeast two hybrid. The results showed that noself-activating activity and toxicity of OsCYP22 bait vector to yeast cells were detected., among which 38 positive colonies were obtained by yeast two hybrid screening. Combining sequencing analysis and bioinformatics methods, 20 candidates that may interact with OsCYP22 were screened. Further validation of the full-length interactions between OsCYP22 and OsCSN5 and OsRUB1 related to the regulation of plant root growth was conducted. The results showed that OsCSN5 interacted with OsCYP22 in yeast. This study provides a theoretical basis for further study of the biological function of OsCYP22.

Key words: rice, OsCYP22, Yeast two-hybird, interaction protein

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