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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 403-411.doi: 10.3724/SP.J.1006.2009.00403

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization of Overexpressed Bx7 Gene(Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

REN Yan12;LIANG Dan2;ZHANG Ping-Ping23;HE Zhong-Hu24;CHEN Jing5;FU Ti-Hua1;XIA Xian-Chun2*   

  1. 1College of Agronomy,Sichuan Agricultural University,Ya'an 625014,China;2National Wheat Improvement Center,Institute of Crop Sciences/National key Facility for Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3 Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;4CIMMYT China Office, Beijing 100081,China;5Institute of Biology,Chinese Academy of Sciences,Chengdu610041,China
  • Received:2008-06-30 Revised:2008-09-03 Online:2009-03-12 Published:2009-01-15
  • Contact: XIA Xian-Chun

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Abstract:

Over-expression of the high molecular weight glutenin subunit (HMW-GS) Bx7 is highly associated with dough strength of wheat (Triticum aestivum L.) flour. A total of 163 Chinese and CIMMYT wheat cultivars and advanced lines were tested by two STS markers and RP-HPLC to understand the presence of HMW-GS gene Bx7OE The results indicated that the markers TaBAC1215C06-F517/R964 and TaBAC1215C06-F24671/R25515 could amplify a 447 bp and a 844 bp PCR fragments, respectively, in the lines with Bx7OE, whereas no PCR products were detected in the lines without Bx7OE. Of the 163 cultivars and lines, specific PCR fragments were amplified in 11 genotypes by the two markers, indicating the presence of Bx7OE in these lines, with a frequency of 6.7%. The results obtained by RP-HPLC were consistent with those revealed by STS markers. These two STS markers could be used to detect the presence of Bx7OE gene in wheat cultivars.

Key words: Common wheat(Triticum aestivum L.), RP-HPLC;STS marker, Marker-assisted selection, Bx7OE

[1]Payne P I, Law C N, Mudd E E. Control by homologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet, 1980, 58: 113–120
[2]Wrigley C W. Giant proteins with flour power. Nature, 1996, 381: 738–739
[3]Gianibelli M C, Larroque O R, MacRitchie F. Biochemical, genetic, molecular characterization of wheat glutenin and its component subunits. Cereal Chem, 2001, 78: 635–646
[4]Payne P I. Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Annu Rev Plant Physiol, 1987, 38: 141–153
[5]Payne P I, Holt L M, Law C N. Structural and genetical studies on the weight subunits of wheat glutenin. Theor Appl Genet, 1981, 60: 229–236
[6]Shewry P R, Halford N G, Tatham A S. High molecular weight subunits of wheat glutenin. J Cereal Sci, 1992, 15: 105–120
[7]Zhu J-B (朱金宝), Liu G-T(刘广田), Zhang S-Z(张树臻). High and low molecular subunits of glutenin and their relationships with wheat quality. Sci Agric Sin (中国农业科学), 1996, 29(1): 34–39 (in Chinese with English abstract)
[8]Radovanovic N, Cloutier S, Brown D, Humphreys D G, Lukow O M. Genetic variance for gluten strength contributed by high molecular weight glutenin proteins. Cereal Chem, 2002, 79: 843–849
[9]Butow B J, Ma W, Gale K R, Cornish G B, Rampling L, Larroque O, Morell M K, Bekes F. Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high molecular weight glutenin allele has a major impact on wheat flour dough strength. Theor Appl Genet, 2003, 107: 1524–1532
[10]Vawser M J, Cornish G B. Overexpression of HMW glutenin subunit Glu-B1 7x in hexaploid wheat varieties (Triticum aestivum L.). Aust J Agric Res, 2004, 55: 577–588
[11]Lukow O M, Forsyth S A, Payne P I. Over-production of HMW glutenin subunits coded on chromosome 1B in common wheat (Triticum aestivum L.). J Genet Breed, 1992, 46: 187–192
[12]D’Ovidio R, Masci S, Porceddu E, Kasarda D. Duplication of the high-molecular weight glutenin subunit gene in bread wheat (Triticum aestivum L.) cultivar Red River 68. Plant Breed, 1997, 116: 525–531
[13]Butow B J, Gale K R, Ikea J, Juhasz A, Bedo Z, Tamas L, Gianibelli M C. Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor Appl Genet, 2004, 109: 1525–1535
[14]Cloutier S, Banks T, Nilmalgoda S. Molecular understanding of wheat evolution at the Glu-B1 locus. In: Proceedings of the International Conference on Plant Genomics and Biotechnology: Challenges and Opportunities, Raipur, India, 2005. p 40
[15]Ragupathy R, Naeem H A, Reimer E, Lukow O M, Sapirstein H D, Cloutier S. Evolutionary origin of the segmental duplication encompassing the wheat Glu-B1 encoding the overexpressed Bx7 (Bx7OE) high molecular weight glutenin subunit. Theor Appl Genet, 2007, 116: 283–296
[16]Jiang N, Bao S, Zhang X, Eddy S R, Wessler S R. Pack-MULE transposable elements mediate gene evolution in plants. Nature, 2004, 431: 569–573
[17]Morgante M, Brunner S, Pea G, Fengler K, Zuccolo A, Rafalski A. Gene duplication and exon shuffling by helitron-like transposon generate intraspecies diversity in maize. Nat Genet, 2005, 37: 997–1002
[18]Liu L, He Z H, Yan J, Zhang Y, Xia X C, Pe?a R J. Allelic variation at the Glu-1 and Glu-3 loci, presence of 1B·1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica, 2005, 142: 197–204
[19]Singh N K, Shepherd K W, Cornish G B. A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci, 1991, 14: 203–208
[20]Payne P I, Lawrence G J. Catalogue of alleles for the complex loci, Glu-A1, Glu-B1 and Glu-D1, which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun, 1983, 11: 29–35
[21]Zhang P-P(张平平), Zhang Y(张勇), Xia X-C(夏先春), He Z-H(何中虎). Protocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein. Sci Agric Sin (中国农业科学), 2007, 40(5): 1002–1009 (in Chinese with English abstract)
[22]Devos K M, Gale M D. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet, 1992, 84: 567–572
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