利用斑茅cDNA芯片研究甘蔗受黑穗病菌侵染后基因差异表达

doi:10.3724/SP.J.1006.2009.00940

Abstract

Abstract:

RNAs of sugarcane leaves with (treatment) or without (control) the infection of U. scitaminea were extracted, subjected to hybridization of cDNA microarray based on E. arundinaceus cDNA sequence and further validated by Real-time qPCR. There were about 101 differentially expressed ESTs (with ratio value ≥2.0 or ≤0.5) among 3 860 genes sets in a microarray plate, with 55 up-regulated, and 46 down-regulated by U. scitaminea. After sequencing and redundant sequences elimination, we totally obtained 36 unique ESTs up-regulated after the infection of U. scitaminea. Among them, 22 were involved in several metabolism pathways, such as photosynthesis, ion transport and nucleotide metabolism, as well as some genes related to transcription factors, proteins synthesis and modulation, and cellular signal transduction. And the function of the 14 remaining ESTs was unknown. In conclusion, the molecular mechanism of sugarcane smut resistance is complex. This investigation would provide an understanding for differentially expressed genes induced by U. scitaminea and set a mode for the systematic research on molecular mechanism of sugarcane responses to biotic and abiotic stress.

Key words: Sugarcane, Ustilago scitaminea, E.arundinaceus, cDNA microarray, Differentially expressed gene

QUE You-Xiong,XU Li-Ping*,LIN Jian-Wei,XU Jing-Sheng,ZHANG Ji-Sen,ZHANG Mu-Qing. Application of E.arundinaceus cDNA Microarray in the Study of Differentially Expressed Genes Induced by U.sitaminea[J].Acta Agron Sin, 2009, 35(5): 940-945.

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Application of E.arundinaceus cDNA Microarray in the Study of Differentially Expressed Genes Induced by U.sitaminea

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