Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1743-1751.doi: 10.3724/SP.J.1006.2012.01743

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Marker-Assisted Selection of HMW-Glutenin 1Dx5+1Dy10 Gene and 1B/1R Translocation for Improving Industry Quality in Common Wheat

ZHANG Yong1,SHEN Xiao-Yong1,ZHANG Wen-Xiang1,CHEN Xin-Min1,YAN Jun1,2,ZHANG Yan1,WANG De-Sen1,WANG Zhong-Wei1,LIU Yue-Fang3,TIAN Yu-Bing1,XIA Xian-Chun1,HE Zhong-Hu1,4,*   

  1. 1 Institute of Crop Sciences / National Wheat Improvement Center / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China; 2 Cotton Research Institute, CAAS, Anyang 455000, China; 3 Wuqing Extension Center for Agricultural Technique, Tianjin 301700, China; 4CIMMYT-China Office, c/o CAAS, Beijing 100081, China
  • Received:2012-03-14 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 何中虎, E-mail: zhhecaas@163.com

Abstract:

Glutenin subunits play an important role in determining processing quality in common wheat. In this study, a total of 125 BC2F4 lines derived from four populations by marker-assisted backcrossing, with Yumai 34, Gaocheng 8901, and Zhongyou 9507 as quality donor and Lunxuan 987, Shi 4185, and Zhoumai 16 as recipients, were used to assess the effect of glutenin subunit 5+10 and 1B/1R translocation on quality parameters including protein content, mixograph mixing time and peak integral. A wide range of variation for all quality parameters in the populations was detected. Lines with 1Dx5+1Dy10 performed significantly longer mixing time and higher peak integral than those with alleles 1Dx2+1Dy12, while the genetic background of parents had a large impact on mixing time and peak integral among 1B/1R translocation lines. Reversed-phase high-performance liquid chromatography (RP-HPLC) revealed that mixing time and peak integral were related with the quantity of glutenin subunit fractions, and significant and positive correlations between mixing time, peak integral and the quantity of LMW-GS were observed, with correlation coefficients ranging from 0.38 to 0.74 (P < 0.05). Lines with 1Dx5+1Dy10 also performed significantly higher quantity of both HMW-GS and LMW-GS than those with alleles 1Dx2+1Dy12, while the effect of alleles at Glu-B3 locus on quantity of HMW-GS was insignificant, and that on quantity of LMW-GS varied among the populations. It would be efficient to select new line through backcross with quality parent as donor and high yield parent as recipient, by marker-assisted selection of Glu-D1d gene and 1B/1R translocation, in combination with field selection on agronomic parameters.

Key words: Common wheat, Processing quality, Subunit 5+10, 1B/1R translocation, Marker-assisted selection

[1]He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民), Zhuang Q-S(庄巧生). Progress of wheat breeding in China and the future perspective. Acta Agron Sin (作物学报), 2011, 37(2): 202-215 (in Chinese with English abastact)



[2]Branlard G, Dardevet M. Diversity of grain proteins and bread wheat quality: I. Correlation between gliadin bands and flour quality characteristics. J Cereal Sci, 1985, 3: 329-343



[3]Payne P I, Nightigale M A, Krattiger A F. The relationship between HMW glutenin subunit composmon and the bread-making quality of British-grown wheat varieties. J Sci Food Agric, 1987, 40: 51-65



[4]Zhang Y, Tang J W, Yan J, Zhang Y L, Zhang Y, Xia X C, He Z H. The gluten protein and interactions between components determine mixograph properties in an F6 recombinant inbred line population in bread wheat. J Cereal Sci, 2009, 50: 219-226



[5]Gupta R B, Shepherd K W. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin: I. Variation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet, 1990, 80: 65-74



[6]Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Wang D-S(王德森), Zhang Y(张艳), Peña R J, Yu Y-X(于亚雄). Effect of allelic variation in HMW and LMW glutenin subunits on the processing auality in common wheat. Sci Agric Sin (中国农业科学), 2004, 37(1): 8-14 (in Chinese with English abastact)



[7]Song J-M(宋健民), Liu A-F(刘爱峰), Wu X-Y(吴祥云), Liu J-J(刘建军), Zhao Z-D(赵振东), Liu G-T(刘广田). Composition and content of high molecular weight glutenin subunits and their relations with wheat quality. Sci Agric Sin (中国农业科学), 2003, 36(2): 128-133 (in Chinese with English abastact)



[8]Liu L(刘丽), Yan J(阎俊), Zhang Y(张艳), He Z-H(何中虎), Peña R J, Zhang L-P(张立平). Allelic variation at the Glu-1 and Glu-3 loci and presence of 1B/1R translocation, and their effects on processing quality in cultivars and advanced lines from autumn-sown wheat regions in China. Sci Agric Sin (中国农业科学), 2005, 38(10): 1944-1950 (in Chinese with English abastact)



[9]Pogna N E, Autran J C, Mellini F, Lafiandra D, Feillet P. Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J Cereal Sci, 1990, 11: 15-34



[10]Gupta R B, Batey I L, MacRitchie F. Relationship between protein composition and functional properties of wheat flours. Cereal Chem, 1992, 69: 125-131



[11]Zhou Y(周阳), He Z-H(何中虎), Zhang G-S(张改生), Xia L-Q(夏兰琴), Chen X-M(陈新民), Gao Y-C(高永超), Jin Z-B(井赵斌), Yu G-J(于广军). Utilization of 1BL/ 1RS translocation in wheat breeding in China. Acta Agron Sin (作物学报), 2004, 30(6): 531-535 (in Chinese with English abastact)



[12]Bustos A D, Rubio P, Jouve N. Molecular characterization of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor Appl Genet, 2000, 100: 1085-1094



[13]Bustos A D, Rubio P, Soler C, Garcia P, Jouve N. Marker assisted selection to improve HMW-glutenins in wheat. Euphytica, 2001, 119: 69-73



[14]D’Ovidio R, Porceddu E, Lafiandra D. PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus. Theor Appl Genet, 1994, 88: 175-180



[15]D’Ovidio R, Masci S, Porceddu E. Development of a set of oligonucleotide primers specific for genes at the Glu-1 complex loci of wheat. Theor Appl Genet, 1995, 91: 189-194



[16]Lei Z S, Gale K R, He Z H, Gianibelli M C, Larroque O, Butow B J. Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. J Cereal Sci, 2006, 43: 94-101



[17]Smith H A, Barlana H S, Ogbonnaya F C. Implementstion of markers in Australian wheat breading. Aust J Agric Res, 2001, 52: 1349-1356



[18]Wang L H, Zhao X L, He Z H, Ma W, Appels R, Peña P J, Xia X C. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor Appl Genet, 2009, 118: 525-539



[19]Wang L H, Li G Y, Peña R J, Xia X C, He Z H. Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). J Cereal Sci, 2010, 51: 305-312



[20]Zhang X F, Liu D C, Yang W L, Liu K F, Sun J Z, Guo X L, Li Y W, Wang D W, Ling H Q, Zhang A M. Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2011, 122: 1503-1516



[21]Zhao X L, Xia X C, He Z H, Le Z S, Appels R, Yang Y, Sun Q X, Ma W. Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat. Theor Appl Genet, 2007, 114: 451-460



[22]Ma W, Zhang W, Gale K R. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica, 2003, 134: 51-60



[23]Zhang X-K(张晓科), Xia X-C(夏先春), Wang Z-W(王忠伟), He X-Y(何心尧), Yang Y(杨燕), He Z-H(何中虎). Establishment of multiplex-PCR for quality traits in common wheat. Acta Agron Sin (作物学报), 2007, 33(10): 1703-1710 (in Chinese with English abastact)



[24]Varghese J P, Struss D, Kazman M E. Rapid screening of selected European winter wheat varieties and segregating population for the Glu-D1d allele using PCR marker. Plant Breed, 1996, 115: 451-454



[25]Xu X-B(徐相波), Liu D-C(刘冬成), Guo X-L(郭小丽), Liu L-K(刘立科), Jia X(贾旭), Zhang X-Q(张相岐), Zhang A-M(张爱民). Identification and marker-assisted selection of HMW-glutenin 1Dx5 gene in wheat. Sci Agric Sin (中国农业科学), 2005, 38(2): 415-419 (in Chinese with English abastact)



[26]Zhang X-K(张晓科), Wei Y-M(魏益民). Method and its effect of rapid transformation of HMW-GS genes with good baking properties into higher yielding wheat line. Sci Agric Sin (中国农业科学), 2005, 38(1): 202-212 (in Chinese with English abastact)



[27]Shen X-Y(申小勇), Yan J(阎俊), Chen X-M(陈新民), Zhang Y(张艳), Li H-L(李慧玲), Wang D-S(王德森), He Z-H(何中虎), Zhang Y(张勇). Relationship among mixograph parameters and farinograph, extensograph, and bread-making quality traits. Acta Agron Sin (作物学报), 2010, 36(6): 1037-1043 (in Chinese with English abastact)



[28]Zhang P P, He Z H, Zhang Y, Xia X C, Liu J J, Yan J, Zhang Y. Pan bread and Chinese white salted noodle qualities of Chinese winter wheat cultivars and their relationship with gluten protein fractions. Cereal Chem, 2007, 84: 370-378



[29]SAS Institute SAS User’s Guide, Statistics. Cary, NC USA: SAS Institute Inc., 2000



[30]Eathington S R, Crosbie T M, Edwards M D, Reiter R S, Bull J K. Molecular markers in a commercial breeding program. Crop Sci, 2007, 47: S154-S163



[31]Liu L(刘丽), Liu J-J(刘建军), He Z-H(何中虎). Effect of allelic variation at the Glu-1 and Glu-3 loci and presence of 1BL/1RS translocation on pan bread and dry white Chinese noodle quality. Sci Agric Sin (中国农业科学), 2004, 37(9): 1265-1273 (in Chinese with English abastact)



[32]He Z H, Liu L, Xia X C, Liu J J, Peña R J. Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem, 2005, 82: 345-350



[33]Graybosch R A, Peterson C J, Hansen L E, Worrall D, Shelton D R, Lukaszewski A. Comparative flour quality and protein characteristics of 1BL/1RS and 1AL/1BS wheat-rye translocation. J Cereal Sci, 1993, 17: 95-106



[34]Darlington H, Fido R, Tatham A S, Jones H, Salmon S E, Shewry P R. Milling and baking properties of field grown wheat expressing HMW subunit transgenes. J Cereal Sci, 2003, 38: 301-306



[35]Butow B J, Ma W, Gale K R, Cornish G B, Rampling L, Larroque O, Morell M K, Békés F. Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high-molecular-weight glutenin allele has a major impact on wheat flour dough strengthn. Theor Appl Genet, 2003, 107: 1524-1532



[36]Lerner S E, Ponzio N R, Rogers W J. Relationship of over-expression of high molecular weight glutenin subunit Bx7 with gluten strength in bread wheat. In: Proceedings of the 10th International Wheat Genetics Symposium, Poestum, Italy, 2003. pp 1360-1362



[37]Zhang P P, He Z H, Zhang Y, Xia X C, Liu J J, Yan J, Zhang Y. Pan bread and Chinese white salted noodle qualities of Chinese winter wheat cultivars and their relationship with gluten protein fraction. Cereal Chem, 2007, 84: 370-378



[38]Wu L-R(吴立人), Meng Q-Y(孟庆玉), Xie S-X(谢水仙), Yang H-A(杨华安), Wang K-N(汪可宁), Yuan W-H(袁文焕), Song W-Z(宋位中), Yang J-X(杨家秀), Li Y-F(李艳芳), Yang S-C(杨世诚). The discovery and studies on physiological races of wheat stripe rust virulent to Lovrin 10 and Lovrin 13. Sci Agric Sin (中国农业科学), 1988, 21(5): 53-58 (in Chinese with English abastact)



[39]Kim W, Johnson J W, Baenziger P S, Lukaszewski A J, Gaines C S, Kim W. Agronomic effect of wheat-rye translocation carrying rye chromatin (1R) from different sources. Crop Sci, 2004, 44: 1254-1258



[40]Kumlay A M, Baenziger P S, Gill K S, Shelton D R, Graybosch R A, Lukaszewski A J, Wesenberg D M. Understanding the effect of rye chromatin in bread wheat. Crop Sci, 2003, 43: 1643-1651

[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] MA Hong-Bo, LIU Dong-Tao, FENG Guo-Hua, WANG Jing, ZHU Xue-Cheng, ZHANG Hui-Yun, LIU Jing, LIU Li-Wei, YI Yuan. Application of Fhb1 gene in wheat breeding programs for the Yellow-Huai Rivers valley winter wheat zone of China [J]. Acta Agronomica Sinica, 2022, 48(3): 747-758.
[3] HAN Yu-Zhou, ZHANG Yong, YANG Yang, GU Zheng-Zhong, WU Ke, XIE Quan, KONG Zhong-Xin, JIA Hai-Yan, MA Zheng-Qiang. Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat [J]. Acta Agronomica Sinica, 2021, 47(6): 1188-1196.
[4] JIN Yi-Rong, LIU Jin-Dong, LIU Cai-Yun, JIA De-Xin, LIU Peng, WANG Ya-Mei. Genome-wide association study of nitrogen use efficiency related traits in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 394-404.
[5] HUANG Yi-Wen, DAI Xu-Ran, LIU Hong-Wei, YANG Li, MAI Chun-Yan, YU Li-Qiang, YU Guang-Jun, ZHANG Hong-Jun, LI Hong-Jie, ZHOU Yang. Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat [J]. Acta Agronomica Sinica, 2021, 47(11): 2080-2090.
[6] ZHANG Ping-Ping,YAO Jin-Bao,WANG Hua-Dun,SONG Gui-Cheng,JIANG Peng,ZHANG Peng,MA Hong-Xiang. Soft wheat quality traits in Jiangsu province and their relationship with cookie making quality [J]. Acta Agronomica Sinica, 2020, 46(4): 491-502.
[7] HU Mao-Long, CHENG Li, GUO Yue, LONG Wei-Hua, GAO Jian-Qin, PU Hui-Ming, ZHANG Jie-Fu, CHEN Song. Development and application of the marker for imidazolinone-resistant gene in Brassica napus [J]. Acta Agronomica Sinica, 2020, 46(10): 1639-1646.
[8] Di JIN,Dong-Zhi WANG,Huan-Xue WANG,Run-Zhi LI,Shu-Lin CHEN,Wen-Long YANG,Ai-Min ZHANG,Dong-Cheng LIU,Ke-Hui ZHAN. Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat [J]. Acta Agronomica Sinica, 2019, 45(6): 807-817.
[9] Ping ZHANG,Yi-Mei JIANG,Peng-Hui CAO,Fu-Lin ZHANG,Hong-Ming WU,Meng-Ying CAI,Shi-Jia LIU,Yun-Lu TIAN,Ling JIANG,Jian-Min WAN. Introducing qSS-9 Kas into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability [J]. Acta Agronomica Sinica, 2019, 45(3): 335-343.
[10] Fang-Ping YANG,Jin-Dong LIU,Ying GUO,Ao-Lin JIA,Wei-E WEN,Kai-Xiang CHAO,Ling WU,Wei-Yun YUE,Ya-Chao DONG,Xian-Chun XIA. QTL mapping of adult-plant resistance to stripe rust in wheat variety holdfast [J]. Acta Agronomica Sinica, 2019, 45(12): 1832-1840.
[11] ZHANG An-Ning,LIU Yi,WANG Fei-Ming,XIE Yue-Wen,KONG De-Yan,NIE Yuan-Yuan,ZHANG Fen-Yun,BI Jun-Guo,YU Xin-Qiao,LIU Guo-Lan,LUO Li-Jun. Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line [J]. Acta Agronomica Sinica, 2019, 45(11): 1764-1769.
[12] Hong-Bo SHAN,Jia-Wen SHI,Ying SHI. Development and Validation of Molecular Marker for Protein Content in Tetraploid Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1095-1102.
[13] Yu-Ling LI,Zheng-Ning JIANG,Wen-Jing HU,Dong-Sheng LI,Jing-Ye CHENG,Xin YI,Xiao-Ming CHENG,Rong-Lin WU,Shun-He CHENG. Mapping QTLs against Leaf Rust in CIMMYT Wheat C615 [J]. Acta Agronomica Sinica, 2018, 44(6): 836-843.
[14] Yu TIAN,Lei YANG,Ying-Hui LI,Li-Juan QIU. Development and Utilization of KASP Marker for SCN3-11 Locus Resistant to Soybean Cyst Nematode [J]. Acta Agronomica Sinica, 2018, 44(11): 1600-1611.
[15] Zhan-Wang ZHU, Deng-An XU, Shun-He CHENG, Chun-Bao GAO, Xian-Chun XIA, Yuan-Feng HAO, Zhong-Hu HE. Characterization of Fusarium Head Blight Resistance Gene Fhb1 and Its Putative Ancestor in Chinese Wheat Germplasm [J]. Acta Agronomica Sinica, 2018, 44(04): 473-482.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!