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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1775-1781.doi: 10.3724/SP.J.1006.2012.01775

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Using Marker-Assisted Selection to Improve the Resistance to Rice Black-Streaked Dwarf Viral Disease of Huaidao 5, an Elite Japonica Rice Cultivar in Jiangsu

LI Ai-Hong1,*,PAN Cun-Hong1,**,DAI Zheng-Yuan1,XIAO Ning1,YU Ling1,LI Yu-Hong1,ZHANG Xiao-Xiang1,ZHANG Hong-Xi1,PAN Xue-Biao2   

  1. 1 Lixiahe Agricultural Research Institute of Jiangsu Province/Yangzhou Station of Nanjing Sub-center of National Rice Improvement Center, Yangzhou 225007, China; 2 Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2012-02-13 Revised:2012-06-20 Online:2012-10-12 Published:2012-07-27
  • Contact: 李爱宏, E-mail: yzlah@126.com

Abstract:

Rice black-streaked dwarf viral disease (RBSDV), a kind of viral disease transmitted by small plant hopper, broke out recently in Jiangsu and Zhejiang provinces of China and posed a serious threat to rice production. Based on mapping of the QTLsfor RBSDV resistance previously, we used the resistant parent Minghui 63 as donor, the susceptible japonica cultivar Huaidao 5 as recurrent parent, and bred a series of Near-isogenic lines (NILs) aiming at two closely linked QTLs on the short arm of Chromosome 6 in rice. Natural inoculation and artificial inoculation results showed that RBSDV incidence rate of NILs was about 25% lower than that of recurrent parent and the resistance of NILs was improved significantly. Agronomic traits of most NILs, such as heading date, plant height, and yield components were similar to those of recurrent parent, indicating that the target chromosome region contains few linkage drags. The strategies for fine mapping the target QTLs and pyramiding more QTLs in the way of breed improvement were also discussed.

Key words: RBSDV, Huaidao 5, MAS, NILs, Resistance evaluation

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