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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 2007-2014.doi: 10.3724/SP.J.1006.2012.02007

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Epistatic and Additive Effects of QTL for Yield-Related Traits Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

ZHAO Fang-Ming1,ZHANG Gui-Quan2,ZENG Rui-Zhen2,YANG Zheng-Lin1,LING Ying-Hua1,SANG Xian-Chun1,HE Guang-Hua1,*   

  1. 1 Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China; 2 Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China
  • Received:2012-03-18 Revised:2012-07-05 Online:2012-11-12 Published:2012-09-10
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

Abstract:

Yield-related traits such as panicle number per plant, thousand grain weight, number of grains per panicle, number of spikelets per panicle and seed setting rate are important agronomic traits in rice. Understanding additive and epistatic effects of QTL for yield-related traits are important to increase rice yield using method of pyramiding breeding with molecular marker assisted selection. In this paper, additive and epistatic effects of QTLs for rice yield-related traits were analyzed using 16 single segment substitution lines (SSSL) and 15 double segment substitution lines (DSSL). A total of thirteen QTLs for yield-related traits were identified on the chromosomes 2, 3, 4, 7, and 10 respectively, containing one for grain yield per plant (GY), one for panicle number (PN), four for thousand grain weight (TGW), four for number of grains per panicle (NGP), two for number of spikelets per panicle (NSP) and one for seed setting rate (SSR). Furthermore, twelve pairs of digenic interactions were detected for yield-related traits. The results showed that pyramiding two QTLs with positive effects (or two QTLs with negative effects) often results in negative epistatic effects (or positive epistatic effects) in DSSL. Whether larger or smaller value of yield-related traits is produced lies on the difference between the absolute value of genetic effect (algebraic sum of additive and epistatic effects) in the DSSL and the largest value of additive effect in the SSSL. These results are important to improve yield by pyramiding favorable QTLs for yield-related traits.

Key words: Rice, Single segment substitution lines, QTL for yield-related traits, Additive effects, Epistatic effects

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