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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 992-998.doi: 10.3724/SP.J.1006.2013.00992

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Mapping of Leaf Pale Yellow-Revertible Mutant pyr1 in Rice

CHENG Xin,REN De-Yong,MA Jiao,ZHU Xiao-Yan,SANG Xian-Chun,LING Ying-Hua,ZHAO Fang-Ming,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China?
  • Received:2012-11-12 Revised:2013-01-15 Online:2013-06-12 Published:2013-03-22

Abstract:

It is important for the further understanding of the relationship between chlorophyll synthesis and degradation pathways and plant photosynthesis to identify and clone leaf color mutant gene. A leaf pale yellow-revertible mutant temporarily designated as pyr1 was obtainted from the progeny of rice (Oryza sativa L.) restorer line Jinhui 10 which was induced by ethyl methane sulfonate (EMS). In the seedling stage, the whole leaf of mutants presented pale and some mutants died. The pyr1 displayed different colors in different growth periods. At the booting stage upper-leaf and leaf margin exhibited yellow. Compared with the wild type, the chlorophyl contents of pyr1 mutant decreased from seedling stage to filling stage. Transmission electronic microscopy observation showed that the structure of cells had no obvious differences between mutant and wild type, but the chloroplast developed abnormally with degradation of the inside and matrix slices. Genetic analysis revealed that the trait was controlled by one recessive gene. With 326 recessive individuals from the F2 segregation population, the PYR1 gene was finally mapped between RM11722 and Ind1 on the long arm of chromosome 1, with an approximate physical distance of 92 kb. These results provide a basis of PYR1 gene cloning by map-based strategy.

Key words: Pale leaf, Rice (Oryza sativa L.), Chloroplast, Gene location

[1]Victor I K, Fabienne P C, Michel H, Pascale C D, Danja S, Karin M, Patrice G, Jonathan D G J, Neil E H, Laurent N. A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell, 1999, 1: 87−99



[2]Chen G, Bi Y R, Li N. EGY1 encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development. Plant J, 2005, 41: 364−375



[3]Leister D. Chloroplast research in the genomic age. Trends Genet, 2003, 19: 47−56



[4]Aluru M R, Rodermel S R. Control of chloroplast redox by the IMMUTANS terminal oxidase. Physiol Plant, 2004, 120: 4−11



[5]Xing C, Wang G X, Huang J L, Wu J Z. Research on chlorophyll mutation of plants and molecular mechanism. Biotechnol Bull, 2008, 5: 10−12



[6]Zhao Y, Wang M L, Zhang Y Z, Du L F, Pan T. A chlorophyll-reduced seedling mutant in oil seed rape Brassica napus for utilization in F1 hybrid production. Plant Breed, 2000, 119: 131−135



[7]Gan S, Amasino R M. Inhibition of leaf senescence by autoregulated production of cytokinin. Science, 1995, 270: 1986−1988



[8]Fambrini M, Castagna A, Vecchia F D. Characterization of a pigment-deficient mutant of sunflower (Helianthus annuus L.) with abnormal chloroplast biogenesis, reduced PS II activity and low endogenous level of abscisic acid. Plant Sci, 2004, 167: 79−89



[9]Parks B M, Quail P H. Phytochrome-deficient hy1 and hy2 long hypocotyls mutants of Arabidopsis are defective in phytochrome chromophore biosynthesis. Plant Cell, 1991, 3: 1177−1186



[10]Agrawal G K, Yamazaki M, Kobayashi M, Hirochika R, Miyao A, Hirochika H. Screening of the rice viviparous mutants generated by endogenous retrotransposon Tos17 insertion. Tagging of a zeaxanthin epoxidase gene and a novel OsTATC gene. Plant Physiol, 2001, 125: 1248−1257



[11]Singh U P, Prithiviraj B, Sarma B K. Development of Erysiphe pisi (powdery mildew) on normal and albino mutants of pea (Pisum sativum L.). J Phytopathol, 2000, 148: 591−595



[12]Honeycut R J, Newhouse K E, Palmer R G. Inheritance and linkage studies of a variegated leaf mutant in soybean. J Hered, 1990, 81: 123−126



[13]Oki S, Gu X, Kofoid K D, Liang G H. A light-intensity sensitive chlorophyll mutant in sorghum. Hereditas, 1997, 126: 239−245



[14]Highkin H R. Chlorophyll studies on barley mutants. Plant Physiol, 1950, 25: 294−306



[15]Wang C, He B, Xun M, Wan J. Tagging and mapping of a gene controlling yellowish-green leaf. Rice Genet Newsl, 2003, 20: 35



[16]Wang J(王军), Wang B-H(王宝和), Zhou L-H(周丽慧), Xu J-F(徐洁芬), Gu M-H(顾铭洪), Liang G-H(梁国华). Genetic analysis and molecular mapping of a new yellow-green leaf gene ygl-2 in rice. Chin J Rice Sci (中国水稻科学), 2006, 20(5): 455−459 (in Chinese with English abstract)



[17]Huang X-Q(黄晓群), Wang P-R(王平荣), Zhao H-X(赵海新), Deng X-J(邓晓建). Genetic analysis and molecular mapping of a novel chlorophyll-deficit mutant gene in rice. Chin J Rice Sci (中国水稻科学), 2007, 21(4): 355−359 (in Chinese with English abstract)



[18]Jung K H, Hur J, Ryu C H, Choi Y, Chung Y Y, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463−472



[19]Lee S, Kim J H, Eun S Y, Lee C H, Hirochika H, An G. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57: 805−818 



[20]Wu Z M, Zhang X, He B. A chlorophyll-deficient rice mutant with impaired chorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29−40



[21]Lichtenthaler H K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol, 1987, 48: 350−382



[22]He R-F(何瑞峰), Ding Y(丁毅), Yu J-H(余金洪), Zu M-S(祖明生). Study on leaf ultrastructure of the thermo-sensitive chlorophyll deficient mutant in rice. J Wuhan Bot Res (武汉植物学研究), 2001, 19(1): 1−5 (in Chinese with English abstract)



[23]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[24]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325



[25]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (Beijing) (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)



[26]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLPs) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597–607



[27]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181



[28]Kosambi D D. The estimation of map distances from recombination values. Ann Hum Genet, 1944, 12: 172–175



[29]Gustafsson A. The mutation system of the chlorophyll apparatus. Lund Univ; Arskr, 1940, 36: 11−40



[30]Guo S-W(郭士伟), Wang Y-F(王永飞), Ma S-M(马三梅), Li X(李霞), Gao D-Y(高东迎). Genetic analysis and fine mapping of a green-revertible albino leaf mutant in rice. Chin J Rice Sci (中国水稻科学), 2011, 25(1): 95−98 (in Chinese with English abstract) 



[31]Zhang X-Q(张向前), Li X-Y(李晓燕), Zhu H-T(朱海涛), Wang T(王涛), Xie X-M(解新明). Identification and candidate gene analysis of stage green-revertible albino mutant in rice (Oryza sativa L.). Chin Sci Bull (科学通报), 2010, 55(23): 2296−2301 (in Chinese with English abstract)



[32]Wang J(王军), Yang J(杨杰), Chen Z-D(陈志德), Fan F-J(范方军), Zhu J-Y(朱金燕), Yang J-H(杨金欢), Zhong W-G(仲维功). Physiological character and gene fine mapping of a virescent mutant v13(t) in rice (Oryza sativa L.). Sci Agric Sin (中国农业科学), 2011, 44(10): 1973−1979 (in Chinese with English abstract)



[33]Chen T, Zhang Y D, Zhao L, Zhu Z, Lin J, Zhang S B, Wang C L. Physiological character and gene mapping in a new green-revertible albino mutant in rice. J Genet Genomics, 2007, 34: 331−338



[34]Zhang L-K(张力科), Li Z-B(李志彬), Liu H-Y(刘海燕), Li R-H(李如海), Chen M-Y(陈满元), Chen A-G(陈爱国), Qian Y-L(钱益亮), Hua Z-T(华泽田), Gao Y-M(高用明), Zhu L-H(朱苓华), Li Z-G(黎志康). Study on morphological structure and genetic mapping of two novel leaf color mutants in rice. Sci Agric Sin (中国农业科学), 2010, 43(2): 223−229 (in Chinese with English abstract) 
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