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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1164-1171.doi: 10.3724/SP.J.1006.2015.01164

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization and Gene Mapping of Rolled Leaf Mutant 28 (rl28) in Rice (Oryza sativa L.)

FENG Ping**,XING Ya-Di**,LIU Song,GUO Shuang,ZHU Mei-Dan,LOU Qi-Jin,SANG Xian-Chun,HE Guang-Hua,WANG Nan*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Received:2015-01-04 Revised:2015-04-02 Online:2015-08-12 Published:2015-05-04
  • Contact: 王楠, E-mail: wangnan_xndx@126.com; Tel: 13752967156 E-mail:fengpingfighting@163.com

Abstract:

Leaves play a very important role in plant development for their function of photosynthesis. Moderate rolling leaves can facilitate the improvement of plant’s population structure and enhance light-use efficiency, which is very important in ideotype breeding. Therefore, the rolled leaf genes which regulate morphology in rice are important for exploring plant type and improving basic research in molecular biology. This study reported a new gene rolled leaf 28 (rl28), which was derived from EMS-treated restorer line Jinhui10. The mutational trait inherited steadily after several generations’ self-crossing. Compared with the wild-type, the leaves of rl28 began to curl along ?the vasculan bundle in medial axis from booting stage, leaf rolling index was significantly higher than that of the wild-type, and leaf angles were less than those of wild-type. Scanning electron microscopy and morphological analysis showed stoma number per 10-5 m2 and stomatal conductance were significantly higher than those of the wild-type, transpiration rate was significantly higher than that of wild-type. Compared with the wild-type, midrib of rl28 was much larger, and the number of the two adjacent vesicular cells decreased. Genetic analysis showed that the mutational trait was controlled by a single recessive nuclear gene. RL28 was finally mapped on chromosome 5 between SSR markers 5-43 and 5-34 with an interval of 90 kb. These results provide a foundation for cloning and function analysis of RL28.

Key words: Rice(Oryza sativa L.), Rolled leaf mutant, Genetic analysis, Gene mapping

[1]罗远章, 赵芳明, 桑贤春, 凌英华, 杨正林, 何光华. 水稻新型卷叶突变体rl12(t)的遗传分析和基因定位. 作物学报, 2009, 35: 1967–1972



Luo Y Z, Zhao F M, Sang X C, Ling Y H, Yang Z L, He G H. Genetic analysis and gene mapping of a novel rolled-leaf mutant RL12(t) in rice. Acta Agron Sin, 2009, 35: 1967–1972 (in Chinese with English abstract)



[2]陈宗祥, 左示敏, 张亚芳, 李磊, 潘雪彪, 马玉银. 水稻卷叶性状遗传及育种应用研究进展. 扬州大学学报, 2010, 31(4): 22–27



Chen Z X, Zuo S M, Zhang Y F, Li L, Pan X B, Ma Y Y. Current progress in genetics research and breeding application of rolled leaf in rice. J Yangzhou Univ, 2010, 31(4): 22–27 (in Chinese with English abstract)



[3]朱德峰, 林贤青, 曹卫星. 不同叶片卷曲度杂交水稻的光合特性比较. 作物学报, 2001, 27: 329–333



 Zhu D F, Lin X C, Cao W X. Comparison of leaf photosynthetic characteristics among rice hybrids with different leaf rolling index. Acta Agron Sin, 2001, 27: 329–333 (in Chinese with English abstract)



[4]易继财, 曹友培, 梅曼彤. 一个辐射诱变的水稻卷叶突变体的特性研究. 核农学报, 2014, 28: 757–764



Yi J C, Cao Y P, Mei M T. Characterization of a 60Co-γ mutated rolled-leaf mutant in rice. J Nucl Agric Sci, 2014, 28: 757–764 (in Chinese with English abstract)



[5]田晓庆, 桑贤春, 赵芳明, 李云峰, 凌英华, 杨正林, 何光华. 水稻卷叶基因RL13的遗传分析和分子定位. 作物学报, 2012, 38: 423–428



Tian X Q, Sang X C, Zhao F M, Li Y F, Ling Y H, Yang Z L, He G H. Genetic analysis and molecular mapping of a rolled leaf gene RL13 in rice (Oryza sativa L.). Acta Agron Sin, 2012, 38: 423–428 (in Chinese with English abstract)



[6]Zhang G H, Xu Q, Zhu X D, Qian Q, Xue H W. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21: 719–735



[7]Shi Z Y, Wang J, Wan X S, Shen G Z, Wang X Q, Zhang J L. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta, 2007, 226: 99–108



[8]Fang L K, Zhao F M, Cong Y F, Sang X C, Du Q, Wang D Z, Li Y F, Ling Y H, Yang Z L, He G H. Rolling-eaf14 is a 2OG-Fe(II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves. Plant Biotechnol J, 2012, 10: 524–532



[9]Hibara K, Obara M, Hayashida E, Abe M, Ishimaru T, Satoh H, Itoh J, Nagato Y. The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice. Dev Biol, 2009, 334: 345–354



[10]Zou L P, Sun X H, Zhang Z G, Liu P, Wu J X, Tian C J, Qiu J L, Lu T G. Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice. Plant Physiol, 2011, 156: 1589–1602



[11]Yang X, Wang Y H, Long Q Z, Huang J X, Wang Y L, Zhou K N, Zheng M, Sun J, Chen H, Chen S H, Jiang L, Wang C M, Wan J M. Overexpression of OsZHD1, a zinc finger homeodomain class homeobox transcription factor, induces abaxially curled and drooping leaf in rice. Planta, 2014, 239: 803–816



[12]Zhao Y D, Christensen S K, Fankhauser C, Cashman J R, Cohen J D, Weigel D, Chory J. A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science, 2001, 291: 306–309



[13]Tobena-Santamaria R, Bliek M, Ljung K, Sandberg G, Mol J M N, Souer E, Koes R. FLOOZY of petunia is a flavin monooxygenase-like protein required for the specification of leaf and flower architecture. Genes Dev, 2002, 16: 753–763



[14]Fujino K, Matsuda Y, Ozawa K, Nishimura T, Koshiba T, Fraaije M W, Sekiguchi H. Narrow leaf 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279: 499–507



[15]高艳红, 吕川根, 王茂青, 王彭, 闫晓燕, 谢坤, 万建明. 水稻卷叶性状QTL的初步定位. 江苏农业学报, 2007, 23(1): 5–10



Gao Y H, Lü C G, Wang M Q, Wang P, Yan X Y, Xie K, Wan J M. QTL mapping for rolled leaf gene in rice. Jiangsu J Agric Sci, 2007, 23(1): 5–10 (in Chinese with English abstract)



[16]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregantion analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[17]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321–4325



[18]桑贤春, 何光华, 张毅, 杨正林, 裴炎. 水稻PCR扩增模板的快速制备. 遗传, 2003, 25: 705–707



Sang X C, He G H, Zhang Y, Yang Z L, Pei Y. The simple gain of templates of rice genomes DNA for PCR. Hereditas (Beijing), 2003, 25: 705–707 (in Chinese with English abstract)



[19]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Genet Genomics, 1996, 252: 597–607



[20]Xiang J J, Zhang G H, Qian Q, Xue H W. SEMI-ROLLED LEAF1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells. Plant Physiol, 2012, 159: 1488–1500



[21]Hong Z, Ueguchi-Tanaka M, Shimizu-Sato S, Inukai Y, Fujioka S, Shimada Y, Takatsuto S, Agetsuma M, Yoshida S, Watanabe Y, Uozu S, Kitanabe H, Ashikari M, Matsuoka M. Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J, 2002, 32: 495–508



[22]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130: 705–718



[23]Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano H Y. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell, 2004, 16: 500–509



[24]Ohmori Y, Toriba T, Nakamura H, Ichikawa H, Hirano H Y. Temporal and spatial regulation of DROOPING LEAF gene expression that promotes midrib formation in rice. Plant J, 2011, 65: 77–86



[25]张静懿. 生长素调控气孔发育的功能和作用分子机理研究. 上海交通大学博士学位论文, 上海, 2014



Zhang J Y. The Molecular Mechanism and Function of Auxin Regulating Stomatal Development. PhD Dissertation of Shanghai Jiaotong University, Shanghai, China, 2014 (in Chinese with English abstract)



[26]Price A H, Young E M, Tomos A D. Quantitative trait loci associated with stomatal conductance, leaf rolling and heading date mapped in upland rice (Oryza sativa). New Phytol, 1997, 137: 83–91



[27]李仕贵, 马玉清, 何平, 黎汉云, 陈英, 周开达, 朱立煌. 一个未知的卷叶基因的识别和定位. 四川农业大学学报, 1998, 16: 391–393



Li S G, Ma Y Q, He P, Li H Y, Chen Y, Zhou K D, Zhu L H. Genetic analysis and mapping the flag leaf roll in rice (Oryza sativa L.). J Sichuan Agric Univ, 1998, 16: 391–393 (in Chinese with English abstract)



[28]Shao Y J, Pan C H, Chen Z X, Zuo S M, Zhang Y F, Pan X B. Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.). Chin Sci Bull, 2005, 50: 2466–2472



[29]方佳, 何勇清, 余敏芬, 郑炳松. 植物生长素响应因子基因的研究进展. 浙江农林大学学报, 2012, 29: 611–616



Fang J, He Y Q, Yu M F, Zheng B S. Recent advances with auxin response factor (ARFs): a review. J Zhejiang A&F Univ, 2012, 29: 611–616 (in Chinese with English abstract)



[30]刘强, 张贵友. 植物转录因子的结构与调控作用. 科学通报, 2000, 45: 1465–1474



Liu Q, Zhang G Y. The structure and regulation in plant transcription factor. Chin Sci Bull, 2000, 45: 1465–1474 (in Chinese with English abstract)



[31]Laity J H, Lee B M, Wright P E. Zinc finger proteins: new insights into structural and functional diversity. Curr Opin Struc Biol, 2001, 11: 39–46

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