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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1844-1857.doi: 10.3724/SP.J.1006.2015.01844

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Brassinosteroids (BRs) on Photosynthetic Matter, Nitrogen Accumulation and Use Efficiency during Grain Filling Stage of Hybrid Japonica

TIAN Xiao-Ya,LIU Xin,WANG Qiang-Sheng*,JIANG Qi,FENG Jin-Xia,ZHANG Hui,DING Yan-Feng   

  1. Key Laboratory of Crop Physiology, Ecology and Production Management, Ministry of Agriculture / Nanjing Agricultural University, Nanjing 210095, China?
  • Received:2015-04-13 Revised:2015-09-06 Online:2015-12-12 Published:2015-09-14
  • Contact: 王强盛, E-mail: qswang@njau.edu.cn, Tel: 025-84395313 E-mail:aefslab@163.com
  • Supported by:

    This research was supported by the Central Finance for Agricultural Innovative Technology Extension (TG(14)027,TG(14)013), the Key Technology R & D Program of Jiangsu Province (BE2013355), the Independent Innovation Fund for Agriculture Science & Technology of Jiangsu Province (CX(14)4072), Agrotechnical Innovation Projects of Jiangsu Province (SXGC[2015]112, SXGC[2014]128, SXGC[2014]251, SXGC[2013]335).

Abstract:

Taking the conventional japonica hybrid rice Changyou 3 and indica-japonica super hybrid rice Yongyou 12 as materials, the experiment analyzed the effects of Brassinosteroids (BRs) on photosynthetic characteristics, dry matter accumulation, nitrogen absorption and utilization, non-structural carbohydrates (NSC) accumulation and the NSC/N ratio of different plant organs during grain filling stage. Results showed that the effect of BRs on photosynthetic indexes presented as Gs>Tr>Pn>Ci. Spraying 2,4-epibrassinolide (eBL) could enhance net photosynthetic rate (Pn) and transpiration rate (Tr), maintain chlorophyll content and intercellular CO2 concentration (Ci), and eventually improve the leaves’ photosynthetic capacity during grain filling stage. It could also increase the amounts of dry matter, nitrogen and NSC accumulation, the proportions and rates of dry matter accumulation, nitrogen absorption and NSC transportation, but reduce the dry matter production efficiency of nitrogen nutrition. The effect of Brz was on the contrary. Spraying eBL improved amounts of the dry matter, NSC, nitrogen and the contribution rate to grains made by nitrogen of the two rice varieties, reduced the contribution rate to grains made by dry matters and NSC after flowering. However, Brassinazole (Brz) decreased transfer amounts and rates of the dry matter, NSC and nitrogen, promoted the contribution rate to grains made by dry matters and NSC. The transfer amounts of NSC and nitrogen of different organs in shoot of the two hybrid rice varieties were shown as eBL>CK>Brz, and early stage of grain filling (EGF) > late stage of grain filling (LGF). The NSC/N of different organs in shoot of the two rice varieties showed an increasing tendency with filling, among which the changes in stem-sheath and panicle were the most significant. Spraying eBL decreased the NSC/N of stem-sheath and leaf, Spraying Brz promoted it, while the effects of BRs on panicle was not clear. Yongyou 12 had a stronger net photosynthetic rate in flag leaves, greater accumulations of dry matter, NSC and nitrogen, larger transfer amounts of NSC and nitrogen in early stage of grain filling, and finally its actual yield was 5.03%-9.32% higher than that of Changyou 3. Spraying eBL showed a diverse rule on the accumulations of dry matter, nitrogen and NSC transportation of the two rice varieties. The inhibiting effect of Brz on dry matter and nitrogen accumulations and NSC transferring was stronger in Yongyou 12 than that of Changyou 3.

Key words: Hybrid rice, Brassinosteroids, Photosynthetic characteristics, Dry matter transportation, N use efficiency, NSC accumulation

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