Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1233-1246.doi: 10.3724/SP.J.1006.2016.01233

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Screening and Identification for Drought Resistance during Germination in Sorghum Cultivars

WU Qi1,ZHOU Yu-Fei1,*,GAO Yue1,ZHANG Jiao1,CHEN Bing-Ru1,2,XU Wen-Juan1,HUANG Rui-Dong1,*   

  1. 1 College of Agronomy, Shenyang Agricultural University, Shenyang110866, China; 2 Institute of Crop Resources, Jilin Academy of Agricultural Sciences, Gongzhuling 130124, China
  • Received:2015-12-10 Revised:2016-05-09 Online:2016-08-12 Published:2016-05-11
  • Contact: 周宇飞, E-mail:zhouyufei2002@aliyun.com;黄瑞冬,E-mail: r_huang@126.com, Tel: 024-88487135 E-mail:597912452@qq.com
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-06-02-02), the General Fund of Department of Education of Liaoning Province(L2014271), and the Postdoctoral Fund of Shenyang Agricultural University (139173).

RichHTML

PDF

3007

Cited

2

Abstract:

Germination is a key stage showing drought resistance in sorghum. The present study was conducted to investigate the responses of 54 different sorghum cultivars (hybrid combinations) to drought stress during germination and screen sorghum cultivars suitable for growing in arid environment. PEG-6000 (–0.4 MPa) was used to simulate the typical drought stress environment in artificial climate chamber. Eight parameters including germination potential, germination rate, germination drought resistance index were measured. The results indicated that the comprehensive factor score functions were suitable for the comprehensive assessment of drought resistance during germination of sorghum cultivars based on principal component analysis (PCA). Based on comprehensive factor scores (Y-value), 54 sorghum cultivars (hybridized combinations) were clustered into four groups, in which 13218A×20982R was the highest drought resistant hybrid, 13 cultivars (hybridized combinations) were drought resistant, e.g. Jiza 133, 23 cultivars were drought sensitive, e.g. Sumac, and 17 cultivars were highly drought sensitive, e.g. Liaoza 35. Results of PCA showed that germination drought resistance index, germination rate and root length can be used to screen a large number of sorghum cultivars with drought resistance.

Key words: Sorghum, Germination, Drought resistance, Principal component analysis, Cluster analysis

[1]山仑, 邓西平, 康绍忠. 我国半干旱地区农业用水现状及发展方向. 水利学报, 2002, 33(9): 27–31
Shan L, Deng X P, Kang S Z. Current situation and perspective of agricultural water used in semiarid area of China. J Hydraulic Engin, 2002, 33(9): 27–31 (in Chinese with English abstract)
[2]朴英华, 何文安. 高粱抗旱性研究进展. 安徽农业科学, 2009, 36: 7910–7913
Piao Y H, He W A. Research advance in drought resistance of sorghum. J Anhui Agric Sci, 2009, 36: 7910–7913 (in Chinese with English abstract)
[3]Francois L E, Donovan T, Maas E V. Salinity effects on seed yield, growth, and germination of grain sorghum. Agron J, 1984, 76: 741–744
[4]Hsiao T C, Fereres E, Acevedo E, Henderson D W. Water stress and dynamics of growth and yield of crop plants. In: Lange O L, Kappen L, Schulze E D, eds. Water and Plant Life. Berlin: Springer-Verlag Berlin Heidelberg, 1976. pp 281–305
[5]张锡梅, 徐勇. 谷子、糜子、高粱、玉米抗旱品种气孔扩散阻力、蒸腾速率、叶水势关系的研究. 干旱地区农业研究, 1987, (3): 80–85
Zhang X M, Xu Y. A study of relation of leaf water potential with resistance of stomic diffusion and evapotranspiration rate of millet, broom corn millet, sorghum and corn. Agric Res Arid Areas, 1987, (3): 80–85 (in Chinese with English abstract)
[6]山仑, 徐炳成. 论高粱的抗旱性及在旱区农业中的地位. 中国农业科学, 2009, 42: 2342–2348
Shan L, Xu B C. Discussion on drought resistance of sorghum and Its status in agriculture in arid and semiarid regions. Sci Agric Sin, 2009, 42: 2342–2348 (in Chinese with English abstract)
[7]Ennajeh M, Vadel A M, Cochard H, Khemira H. Comparative impacts of water stress on the leaf anatomy of a drought-resistant and a drought-sensitive olive cultivar. J Hort Sci Biotechnol, 2010, 85: 289
[8]Tuberosa R, Salvi S. Genomics-based approaches to improve drought tolerance of crops. Trends Plant Sci, 2006, 11: 405–412
[9]Arjenaki F G, Dehaghi M A, Jabbari R, Arjenaki M A. Effects of priming on seed germination of marigold (Calendula officinalis L.). Adv Environ Biol, 2011, 5: 276–280
[10]刘自刚, 沈冰, 张雁. 桔梗种子萌发对低温、干旱及互作胁迫的响应. 生态学报, 2013, 33: 2615–2622
Liu Z G, Shen B, Zhang Y. Morphological responses to temperature，drought stress and their interaction during seed germination of Platycodon grandiflorum. Acta Ecol Sin, 2013, 33: 2615–2622 (in Chinese with English abstract)
[11]裴帅帅, 尹美强, 温银元, 黄明镜, 张彬, 郭平毅, 王玉国, 原向阳. 不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价. 核农学报, 2014, 28: 1897–1904
Pei S S, Yin M Q, Wen Y Y, Huang J M, Zhang B, Guo P Y, Wang Y G, Yuan X Y. Physiological response of foxtail millet to drought stress during seed germination and drought resistance evaluation. J Nucl Agric Sci, 2014, 28: 1897–1904 (in Chinese with English abstract)
[12]陈新, 宋高原, 张宗文, 吴斌. PEG-6000胁迫下裸燕麦萌发期抗旱性鉴定与评价. 植物遗传资源学报, 2014, 15: 1188–1195
Chen X, Song G Y, Zhang Z W, Wu B. Identification and evaluation of drought resistance of naked oat (Aveua nuda L.) under PEG-6000 stress at germination stage. J Plant Genet Resourc, 2014, 15: 1188–1195 (in Chinese with English abstract)
[13]孙璐, 周宇飞, 汪澈, 肖木辑, 陶冶, 许文娟, 黄瑞冬. 高粱品种萌发期耐盐性筛选与鉴定. 中国农业科学, 2012, 45: 1714–1722
Sun L, Zhou Y F. Wang C, Tao Y, Xu W J, Huang R D. Screening and identification of sorghum cultivars for salinity tolerance during germination. Sci AgricSin, 2012, 45(9): 1714–1722 (in Chinese with English abstract)
[14]李丰先, 周宇飞, 王艺陶, 孙璐, 白薇, 闫彤, 许文娟, 黄瑞冬. 高粱品种萌发期耐碱性筛选与综合鉴定. 中国农业科学, 2013, 46: 1762–1771
Li F X, Zhou Y F, Wang Y T, Sun L, Bai W, Yan T, Xu W J, Huang R D. Screening and identification of sorghum cultivars for alkali tolerance during germination. Sci Agric Sin, 2013, 46: 1762–1771 (in Chinese with English abstract)
[15]Berkat O, Briske D D. Water potential evaluation of three germination substrates utilizing polyethylene glycol 20,000. Agron J, 1982, 74: 518–521
[16]张立军, 樊金娟, 阮燕晔, 关义新. 聚乙二醇在植物渗透胁迫生理研究中的应用. 植物生理学通讯, 2004, 40: 361–364
Zhang L J, Fan J J, Ruan Y Y, Guan Y X. Application of polyethylene glycol in the study of plant osmotic stress physiology. Plant Physiol Commun, 2004, 40: 361–364 (in Chinese with English abstract)
[17]赫福霞, 李柱刚, 阎秀峰, 王秀君. 渗透胁迫条件下玉米萌芽期抗旱性研究. 作物杂志, 2014, (5): 144–147
He F X, Li Z G, Yan X F, Wang X J. Effect of osmotic stress on drought resistance of maize at germination stage. Crops, 2014, (5): 144–147 (in Chinese with English abstract)
[18]杨子光, 张灿军, 冀天会, 孟丽梅, 郭军伟, 张坷. 小麦抗旱性鉴定方法及评价指标研究Ⅳ萌发期抗旱指标的比较研究. 中国农学通报, 2008, 23(12): 173–176
Yang Z G, Zhang C J, Ji T H, Meng M L, Guo J W, Zhang K. Study on resistance drought identify method and evaluation index of wheat IV the comparative study on resistance drought index of wheat in germination. Chin Agric Sci Bull, 2008, 23(12): 173–176 (in Chinese with English abstract)
[19]敬礼恒, 陈光辉, 刘利成, 何姝. 水稻种子萌发期的抗旱性鉴定指标研究. 杂交水稻, 2014, 3: 26
Jing H L, Chen G H, Liu L C, He S. Studies on identification indices of drought resistance of rice in deed germination. Hybrid Rice, 2014, 3: 26 (in Chinese with English abstract)
[20]刘桂红, 王珏, 杜金哲, 管延安. 谷子萌芽期抗旱性鉴定研究. 中国农学通报, 2013, 29(3): 86–91
Liu G H, Wang J, Du J Z, Guan Y A. Study on drought resistance of millet in seeding emergence stage. Chin Agric Sci Bull, 2013, 29(3): 86–91 (in Chinese with English abstract)
[21]安永平, 强爱玲, 张媛媛, 张文银, 曹桂兰, 韩龙植. 渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究. 植物遗传资源学报, 2006, 4: 421–426
An Y P, Qiang A L, Zhang Y Y, Zhang W Y, Han L Z. Study on characteristics of germination and drought-resistance index by osmotic stress in rice. J PlantGenet Resourc, 2006, (4): 421–426 (in Chinese with English abstract)
[22]徐蕊, 王启柏, 张春庆, 吴承来. 玉米自交系抗旱性评价指标体系的建立. 中国农业科学, 2009, 42: 72–84
Xu R, Wang Q B, Zhang C Q, Wu C L. Drought-resistance evaluation system of maize inbred. Sci Agric Sin, 2009, 42: 72–84 (in Chinese with English abstract)
[23]齐华, 许晶, 孟显华, 王晓波, 刘明, 管宏军. 水分胁迫下燕麦萌芽期抗旱指标的研究. 种子, 2009, (7): 7–10
Qi H, Xu J, Meng X H, Wang X B, Liu M, Guan H J. Studies on the drought resistance index of oat at germination stage under water stress. Seed, 2009, (7): 7––10 (in Chinese with English abstract)
[24]石永红, 万里强, 刘建宁, 王运琦, 郭锐, 吴欣明, 李向林. 多年生黑麦草抗旱性主成分及隶属函数分析. 草地学报, 2010, 18: 669–672
Shi Y H, Wan L Q, Liu J N, Wang Y Q, Guo R, Wu X M, Li X L. Analysis of the principal components and the subordinate function of Lolium perenne drought resistance. Acta Agrest Sin, 2010, 18: 669–672 (in Chinese with English abstract)
[25]周宇飞, 王德权, 陆樟镳, 王娜, 王艺陶, 李丰先, 许文娟, 黄瑞冬. 干旱胁迫对持绿性高粱光合特性和内源激素ABA, CTK含量的影响. 中国农业科学, 2013, 47: 655–663
Zhou Y F, Wang D Q, Lu Z B, Effects of drought stress on photosynthetic characteristics and endogenous hormone ABA and CTK contents in green-stayed sorghum. Sci Agric Sin, 2013, 47: 655–663 (in Chinese with English abstract)
[26]周宇飞, 王德权, 陆樟镳, 王娜, 王艺陶, 李丰先, 许文娟, 黄瑞冬. 干旱胁迫对持绿性高粱叶片渗透调节及叶绿体超微结构的影响. 应用生态学报, 2013, 24: 22
Zhou Y F, Wang D Q, Lu Z B, Wang N, Wang Y T, Li F X, Xu W J, Huang R D. Impacts of drought stress on leaf osmotic adjustment and chloroplast ultrastructure of stay-green sorghum. Chin Appl Ecol, 2013, 24: 2545–2550 (in Chinese with English abstract)
[27]邵艳军, 山仑, 李广敏. 干旱胁迫与复水条件下高粱, 玉米苗期渗透调节及抗氧化比较研究. 中国生态农业学报, 2006, 14: 68–70
Shao Y J, Shan L, Li G M. Comparison of osmotic regulation and antioxidation between sorghum and maize seedlings under soil drought stress and water recovering conditions. Chin J Eco-Agric, 2006, 14: 68–70 (in Chinese with English abstract)
[28]张美俊, 杨武德, 乔治军, 冯美臣, 王冠, 段云, 陈凌. 不同糜子品种萌发期对干旱胁迫的响应及抗旱性评价. 草地学报, 2013, 21: 302–307
Zhang M J, Yang W D, Qiao Z J, Feng M C, Wang G, Duan Y, Chen L. Resistance evaluation and response of 16 millet varieties at germination stage to drought stress. Acta Agres Sin, 2013, 21: 302–307 (in Chinese with English abstract)
[29]伏兵哲, 兰剑, 李小伟, 高雪芹. PEG-6000 干旱胁迫对16个苜蓿品种种子萌发的影响. 种子, 2012, 31(4): 10–14
Fu B Z, Lan J, Li X W, Gao X Q. Effects of PEG-6000 drought stress on seed germination of 16 varieties of alfalfa. Seed, 2012, 31(4): 10–14 (in Chinese with English abstract)
[30]罗音, 王玉军, 谢胜利, 赵新西, 杨兴洪, 王玮. 等渗水分与盐分胁迫对烟草种子萌发的影响及外源甜菜碱的保护作用. 作物学报, 2005, 31: 1029–1034
Luo Y, Wang Y J, Xie S L, Zhao X X, Yang, X H, Wang W. Effects of isotonic water and salt stress on seed germination of tobacco and protective function of exogenous glycine betaine. Acta Agron Sin, 2005, 31: 1029–1034 (in Chinese with English abstract)
[31]张玉梅, 林琪, 姜雯, 张延胜, 李忠. 渗透胁迫条件下不同抗旱性小麦品种萌发期生理生化指标的变化. 麦类作物学报, 2006, 26(6): 125–130
Zhang Y M, Lin Q, Jiang W, Zhang Y S, Li Z. Effect of osmotic stress on physiological and biochemical indices at germination Stage of different drought-resistant wheat. J Triticeae Crops, 2006, 26(6): 125–130 (in Chinese with English abstract)
 
[1] Song Song-Quan, Tang Cui-Fang, Liang Yu-Rong, Cheng Hong-Yan, Wang Wei-Qing. Research progress in biosynthesis and action mechanism of sRNAs and their regulation on seed development, dormancy and germination [J]. Acta Agronomica Sinica, 2026, 52(4): 959-981.
[2] Xu Jian-Xia, Ding Yan-Qing, Cao Ning, Cheng Bin, Gao Xu, Li Wen-Zhen, Wang Ruo-Ruo, Wang Lei, Zhang Li-Yi. Phenotypic diversity analysis and comprehensive evaluation of 397 sorghum germplasm resources in Guizhou, China [J]. Acta Agronomica Sinica, 2026, 52(4): 1073-1087.
[3] Qi Qing-Song, Niu Xiang-Yu, Liu Bing-Ke, Kang Lu, Wang Chen, Feng De-Shun. Salt tolerance identification, screening and salt tolerance index evaluation of wheat-Thinopyrum intermedium radiation mutagenesis germplasm at germination and seedling stage [J]. Acta Agronomica Sinica, 2026, 52(2): 389-404.
[4] Huang Li-Xia, Zhang Wei-Wei, Zhen Yi-Yue, Wang Qiu-Bao, Tian Hong-Ling, Li Guo-Dong, Liu Long-Long, Zhang Li-Jun. Evaluation of salinity tolerance and germplasm screening of buckwheat during germination under salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 459-479.
[5] Wang Fei-Fei, Zhang Sheng-Zhong, Yang Gui-Hua, Miao Hua-Rong, Hu Xiao-Hui, Zhang Ze-Lin, Liu Sha-Sha, Qiao Li-Xian, Shan Shi-Hua, Chen Jing. Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage [J]. Acta Agronomica Sinica, 2026, 52(1): 279-294.
[6] FU Jiang-Peng, LIU Fa-Cai, YAN Bao-Qin, WANG Yong-Dong, LI Li-Li, WEI Wei, ZHOU Ying-Xia. Effect of replacing common urea with controlled-release fertilizer on dry matter accumulation, partitioning, yield, and quality of sorghum in dryland farming [J]. Acta Agronomica Sinica, 2025, 51(9): 2501-2513.
[7] YIN Li-Na, ZHANG Rui, CHEN Guo-Huan, BAI Lei, LI Jun, GUO Hua-Chun, YANG Fang. Comparison of wound healing capacity of tubers of different potato varieties [J]. Acta Agronomica Sinica, 2025, 51(9): 2399-2411.
[8] LI Yun-Xiang, GUO Qian-Qian, HOU Wan-Wei, ZHANG Xiao-Juan. Genome-wide association analysis of drought resistance traits in wheat seedlings introduced from ICARDA [J]. Acta Agronomica Sinica, 2025, 51(9): 2387-2398.
[9] HE Peng-Xu, YAO Li-Rong, CHEN Yuan-Ling, YAN Yan, ZHANG Hong, WANG Jun-Cheng, LI Bao-Chun, YANG Ke, SI Er-Jing, MENG Ya-Xiong, MA Xiao-Le, WANG Hua-Jun. Differences and correlations in physiological and molecular mechanisms of barley germination under drought stress [J]. Acta Agronomica Sinica, 2025, 51(9): 2412-2432.
[10] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[11] WEN Xuan, ZHONG Xiu-Li, WANG Shang-Wen, JIN Tao, PENG Jun, LIU En-Ke. Screening of low nitrogen tolerant germplasm in seedling highland barley based on tolerance index and comprehensive evaluation of different nitrogen efficiency types [J]. Acta Agronomica Sinica, 2025, 51(7): 1949-1958.
[12] YIN Yu-Meng, WANG Yan-Nan, KANG Zhi-He, QIAO Shou-Chen, BIAN Qian-Qian, LI Ya-Wei, CAO Guo-Zheng, ZHAO Guo-Rui, XU Dan-Dan, YANG Yu-Feng. Cloning and functional analysis of glutathione S-transferase gene IbGSTU7 in sweetpotato [J]. Acta Agronomica Sinica, 2025, 51(7): 1736-1746.
[13] SONG Song-Quan, TANG Cui-Fang, CHENG Hong-Yan, WANG Cheng-Liang, YUAN Liang-Bing, ZUO Sheng. Endosperm development of cereal crops and its role in seed dormancy and germination [J]. Acta Agronomica Sinica, 2025, 51(5): 1133-1155.
[14] JIN Xin-Xin, SONG Ya-Hui, SU Qiao, YANG Yong-Qing, LI Yu-Rong, WANG Jin. Identification and comprehensive evaluation of drought resistance in high oleic acid Jihua peanut varieties [J]. Acta Agronomica Sinica, 2025, 51(3): 797-811.
[15] JIANG You, MA Xue-Rong, ZHANG Bo, LI Chen-Jian. Evaluation of salt tolerance and screening of salt-tolerant germplasm of Sorghum sudanese during seed germination period [J]. Acta Agronomica Sinica, 2025, 51(3): 835-844.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd