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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (10): 1429-1436.

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of TaMYB86-Overexpressing Transgenic Wheat Lines with Resistance to Common Root Rot

SHAN Tian-Lei,HONG Yan-Tao,DU Li-Pu,XU Hui-Jun,WEI Xue-Ning,ZHANG Zeng-Yan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-03-10 Revised:2016-07-11 Online:2016-10-12 Published:2016-08-01
  • Contact: E-mail: zhangzengyan@caas.cn, Tel: 010-82108781 E-mail:tlshan8023@163.com
  • Supported by:

    The study was supported by the National Major Project for Developing New GM Crops (2016ZX08002-001-004) and the National Natural Science Foundation of China (31471494).

Abstract:

Wheat common root rot, mainly caused by Bipolaris sorokiniana, is a difficultly prevent soil-borne disease of wheat (Triticum aestivum L.) worldwide. TaMYB86, a B. sorokiniana -induced wheat MYB gene, encodes a MYB transcription factor. We constructed the TaMYB86 overexpression vector pUbi:MYC-TaMYB86 and introduced TaMYB86 into Yangmai 16 via the particle bombardment. The TaMYB86 transgenic wheat lines on generations of T0–T3 were underwent by molecular characteristics analysis and disease resistance evaluation. The PCR and quantitative RT-PCR results showed that the alien TaMYB86 was introduced into three transgenic wheat lines, and the relative transcriptional level of TaMYB86 was apparently higher in transgenic wheat lines than in non-transformed Yangmai 16. As Western blot results presented, the introduced MYC-TaMYB86 gene was translated into the MYC-TaMYB86 protein in the three overexpressing transgenic lines, but not in non-transformed Yangmai 16. The infection types and disease indexes of three TaMYB86 transgenic wheat lines were significantly lower than those of non-transformation Yangmai 16 (t-test, P < 0.01). The transcript levels of 3 wheat defense genes (PR10, PR17c, and Chit1) were significantly elevated in three transgenic wheat lines than in the non-transformed Yangmai 16. These results indicate that overexpression of TaMYB86 enhances significantly resistance to B. sorokiniana in transgenic wheat lines and TaMYB86 plays a positive role in defense response to B. sorokiniana.

Key words: Triticum aestivum, MYB transcription factor, Defense response, Common root rot, Transgene

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