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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (02): 236-244.doi: 10.3724/SP.J.1006.2018.00236

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Sequence Characteristics and Expression Analysis of Potassium Transporter Gene GhHAK5 in Upland Cotton (Gossypium hirsutum L.)

Mao-Ni CHAO1, Qing-Yu WEN2, Zhi-Yong ZHANG1, Gen-Hai HU1, Jin-Bao ZHANG1, Guo WANG1, Qing-Lian WANG1,*()   

  1. 1 Henan Institute of Science and Technology / Henan Collaborative Innovation Center of Modern Biological Breeding / Henan Key Laboratory for Molecular Ecology and Germplasm Innovation of Cotton and Wheat, Xinxiang 453003, Henan, China
    2 Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2017-04-25 Accepted:2017-09-10 Online:2018-02-12 Published:2017-10-27
  • Contact: Qing-Lian WANG E-mail:wangql985@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31601347, 31571600), the Henan Scientific and Technological Research Program (172102110022), Key Research Projects of Henan Higher Education Institutions (15A210001), and High Level Talent Program of Henan Institute of Science and Technology (2014004).

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Abstract:

KUP/HAK/KT potassium transporter gene family plays an important role in the absorption of K+ in plants. Identification and cloning of potassium transporter genes in cotton are helpful to improve potassium absorption, and facilitate the increase of yield and quality in cotton. Based on the genome sequence of upland cotton, we identified the potassium transporter gene GhHAK5 by homologous cloning, and amplified its CDS sequence from upland cotton variety Baimian 1. The CDS of GhHAK5 was 2451 bp, encoding a protein with 816 amino acids. The molecular weight and isoelectric point of this protein were 91.23 kD and 8.15, respectively. The GhHAK5 protein contained a conserved domain “K-trans” (Pfam02705) and a symbolic amino acid sequence GXXXGDXXXSPLY of KUP/HAK/KT family gene, and had 11 transmembrane domains. Phylogenetic tree analysis demonstrated that GhHAK5 had a close relationship with AtHAK5, followed by OsHAK5, and they all belong to Cluster I evolutionary cluster. The subcellular localization experiment indicated that GhHAK5 was located in plasma membrane, which is consistent with its function as a potassium transporter involved in K+ uptake. The expression of GhHAK5 was high in roots, but low in leaf, stem, petal, fiber and sepal, and induced by low potassium environment. These results provide a foundation for understanding the function of GhHAK5 and breeding varieties with high potassium efficiency in cotton.

Key words: upland cotton, potassium transporter gene, subcellular localization, low potassium, gene expression

Fig. 1

Sequence analysis of GhHAK5 protein in upland cotton (Gossypium hirsutum L.) A: conserved domain of GhHAK5; B: symbolic amino acids sequence (underlined) of KUP/HAK/KT potassium transporter family harbored in GhHAK5; C: transmembrane domain prediction of GhHAK5."

Fig. 2

Phylogenetic relationships between GhHAK5 protein and KUP/HAK/KT family members in Arabidopsis and rice The hollow and solid small circles indicate the KUP/HAK/KT members of rice and Arabidopsis, respectively."

Fig. 3

Subcellular localization of GhHAK5 protein in upland cotton (Gossypium hirsutum L.) a, d: fluorescence images; b, e: bright-field images; c, f: overlap of fluorescence and bright-field images."

Fig. 4

Expression level of GhHAK5 gene in upland cotton (Gossypium hirsutum L.) A: organ-specific expression of GhHAK5 in upland cotton (error bars represent standard errors of three independent repetitions); B: expression of GhHAK5 in roots under low potassium (CK: normal potassium; LK: low potassium; **: P < 0.01; error bars represent standard errors of three independent repetitions)."

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